Large-scale preparation of sequence-ready bacterial artificial chromosome DNA using QIAGEN® columns

BioTechniques

Volumn 27, Issue 1, 1999, Pages 72-74

  Kirschner, Lawrence S ^a   Stratakis, Constantine A ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; AUTOMATION; CHROMOSOME; DNA DETERMINATION; DNA SEQUENCE; ESCHERICHIA COLI; NONHUMAN;

BASE SEQUENCE; CHROMOSOMES, BACTERIAL; CLONING, MOLECULAR; DNA RESTRICTION ENZYMES; DNA, BACTERIAL; ELECTROPHORESIS, AGAR GEL; MOLECULAR SEQUENCE DATA; REAGENT KITS, DIAGNOSTIC; SEQUENCE ANALYSIS, DNA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0033030196     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (5)

1. Birnboim, H.C. and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
2. Kim, U.-J., B.W. Birren, T. Slepak, V. Mancino, C. Boysen, H.-L. Kang, M.I. Simon and H. Shizuya. 1996. Construction and characterization of a human bacterial artificial chromosome library. Genomics 34:213-218.
3. Shizuya, H., B.W. Birrin, U.-J. Kim, V. Mancino, T. Slepak, Y. Tachiiri and M.I. Simon. 1992. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA 89:8794-8797.
4. Sinnett, D., C. Richer and A. Baccichet. 1998. Isolation of stable bacterial artificial chromosome DNA using a modified alkaline lysis method. BioTechniques 24:752-754.
5. Thomas, N.R., S. Koshy, M. Simsek and A.K. Abraham. 1988. A precaution when preparing very large plasmids by alkaline lysis procedure. Biotechnol. Appl. Biochem. 10:402-407.