Isolation of stable bacterial artificial chromosome DNA using a modified alkaline lysis method

BioTechniques

Volumn 24, Issue 5, 1998, Pages 752-754

  Sinnett, Daniel ^a   Richer, Chantal ^b   Baccichet, Annie ^b  

^a UNIVERSITY OF MONTREAL   (Canada)

^b UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; CHROMOSOME; DNA DETERMINATION; DNA SEQUENCE; GENE INSERTION; PLASMID;

EID: 0031945079     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98245bm12     Document Type: Article

References (4)

1. Birnboim, B.C. and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
2. Kim, U.-J., B.W. Birren, T. Slepak, V. Mancino, C. Boysen, H.-L. Kang, M.I. Simon and H. Shizuya. 1996. Construction and characterization of a human bacterial artificial chromosome library. Genomics 34:213-218.
3. Shizuya, H., B. Birren, U.-J. Kim, V. Mancino, T. Slepak, Y. Tachiiri and M.I. Simon. 1992. Cloning and stable maintenance of 300- kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA 59:8794-8797.
4. Thomas, N.R., S. Koshy, M. Simsek and A.K. Abraham. 1988. A precaution when preparing very large plasmids by alkaline lysis procedure. Biotechnol. Appl. Biochem. 10:402-407.