Purification and partial characterization of three isoforms of serine hydroxymethyltransferase from Crithidia fasciculata

Molecular and Biochemical Parasitology

Volumn 98, Issue 2, 1999, Pages 187-201

  Capelluto, Daniel G S ^a   Hellman, Ulf ^b   Cazzulo, Juan J ^c   Cannata, Joaquín J B ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b LUDWIG INSTITUTE FOR CANCER RESEARCH   (Sweden)

^c INSTITUTO DE INVESTIGACIONES BIOTECNOLÓGICAS   (Argentina)

Author keywords

Crithidia fasciculata; Molecular forms; Purification; Pyridoxal 5' phosphate; Serine hydroxymethyltransferase

Indexed keywords

CYSTEINE; GLYCINE HYDROXYMETHYLTRANSFERASE; HYDROXYLAMINE; ISOENZYME; PYRIDOXAL 5 PHOSPHATE; SERINE;

ANIMAL EXPERIMENT; ARTICLE; CELLULAR DISTRIBUTION; CRITHIDIA FASCICULATA; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; MICHAELIS MENTEN KINETICS; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; CELL COMPARTMENTATION; CRITHIDIA FASCICULATA; CYTOSOL; GLYCINE HYDROXYMETHYLTRANSFERASE; ISOENZYMES; KINETICS; MITOCHONDRIA; MOLECULAR WEIGHT; ORGANELLES; PYRIDOXAL PHOSPHATE; SUBCELLULAR FRACTIONS; SUBSTRATE SPECIFICITY;

CHOANOFLAGELLIDA; CRITHIDIA FASCICULATA;

EID: 0033020536     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(98)00166-2     Document Type: Article

References (51)

1. Mc Neil J., Bognar A., Pearlman R. In vivo analysis of folate coenzymes and their compartmentation in Saccharomyces cerevisiae. Genetics. 142:1996;371-381.
2. Cossins E., Chen L. Folates and one-carbon metabolism in plants and fungi. Phytochemistry. 45:1997;437-452.
3. Shane B. Folylpolyglutamate synthesis and role in the regulation of one-carbon metabolism. Vit. Horm. 45:1989;263-335.
4. Schirch L. Serine hydroxymethyltransferase. Rel. Areas. Bioch. Biol. Mol. 53:1982;83-112.
5. Sukanya N, Vijaya M, Savithri HS, Radhakrishnan AN, Appaji Rao N. Serine hydroxymethyltransferase from Mung Bean (Vigna radiata) is not a pyridoxal-5'-phosphate-dependent enzyme. Plant Physiol 1991;95:351-7.
6. Appling D. Comparmentation of folate-mediated one-carbon metabolism in eukaryotes. FASEB. J. 5:1991;2645-2651.
7. Garrow T., Brenner A., Whitehead M., Chen X.N., Duncan R., Korenberg J., Shane B. Cloning of human cDNAs encoding mitochondrial and cytosolic serine hydroxymethyltransferases and chromosomal localization. J. Biol. Chem. 268:1993;11910-11916.
8. Besson V., Neuberger M., Rébeille F., Douce R. Evidence for three serine hydroxymethyltransferases in green leaf cells: purification and characterization of the mitochondrial and chloroplastic isoforms. Plant. Physiol. Biochem. 33:1995;665-673.
9. Sakamoto M., Masuda T., Yanagimoto Y., Nakano Y., Kitaoka S., Tanigawa Y. Purification and characterization of serine hydroxymethyltransferase from mitochondria of Euglena gracilis Z. Biosc. Biotech. Biochem. 60:1996;1941-1944.
10. Artigues A., Birkett A., Schirch V. Evidence for the in vivo deamidation and isomerization of an asparaginyl residue in cytosolic serine hydroxymethyltransferase. J. Biol. Chem. 265:1990;4853-4858.
11. Turner S., Ireland R., Morgan C., Rawsthorne S. Identification and localization of multiple forms of serine hydroxymethyltransferase in pea (Pisum sativum) and characterization of a cDNA encoding a mitochondrial isoform. J. Biol. Chem. 267:1992;13528-13534.
12. Nosei C., Avila J.L. Serine hydroxymethyltransferase activity in Trypanosoma cruzi, Trypanosoma rangeli, and american Leishmania spp. Comp. Biochem. Physiol. 81B:1985;701-704.
13. Reche P., Arrebola R., Olmo A., Santi D., Gonzalez-Pacanowska D., Ruiz Perez L. Cloning and expression of the dihydrofolate reductase-thymidylate synthase gene from Trypanosoma cruzi. Mol. Biochem. Parasitol. 65:1994;247-258.
14. Gamarro F, Yu P, Zhao J, Edman U, Greene P, Santi D. Trypanosoma brucei dihydrofolate reductase-thymidylate synthase: gene isolation and expression and characterization of the enzyme. Mol Biochem Parasitol 1985;72:11-22.
15. Grumont R., Washtein W.L., Caput D., Santi D. Bifunctional thymidylate synthase-dihydrofolate reductase from Leishmania tropica. Sequence homology with the corresponding monofunctional proteins. Proc. Natl. Acad. Sci. USA. 83:1986;5387-5391.
16. Ferone R., Roland S. Dihydrofolate reductase: thymidylate synthase a binfuctional polypeptide from Crithidia fasciculata. Proc. Natl. Acad. Sci. USA. 77:1980;5802-5806.
17. 13C] glucose metabolism in Trypanosoma cruzi. Eur. J. Biochem. 192:1990;363-368.
18. Hammond D.J., Gutteridge W.E. Purine and pyrimidine metabolism in the Trypanosomatidae. Mol. Biochem. Parasitol. 13:1984;243-261.
19. Tiselius A., Hjertén S., Levin O. Protein chromatography on calcium phosphate columns. Arch. Biochem. Biophys. 65:1956;132-155.
20. Cannata J.J.B., Valle E., Docampo R., Cazzulo J.J. Subcellular localization of phosphoenolpyruvate carboxykinase in the trypanosomatids Trypanosoma cruzi and Crithidia fasciculata. Mol. Biochem. Parasitol. 6:1982;151-160.
21. Cazzulo J.J., Valle E., Docampo R., Cannata J.J.B. Intracellular distribution of carbon dioxide-fixing enzymes in Trypanosoma cruzi and Crithidia fasciculata. J. Gen. Microbiol. 117:1980;271-274.
22. Andrews P. The gel filtration behavior of proteins related to their molecular weigths over a wide range. Biochem. J. 96:1965;596-606.
23. Taylor R., Weissbach H. Radioactive assay for serine transhydroxymethylase. Anal. Biochem. 13:1965;80-84.
24. Salvarrey M.S., Cannata J.J.B., Cazzulo J.J. Phosphoenolpyruvate carboxykinase from the moderate halophile Vibrio costicola: purification, physicochemical properties and the effect of univalent-cation salts. Biochem. J. 260:1989;221-230.
25. Bensadoun A., Weinstein D. Assay of proteins in the presence of interfering materials. Anal. Biochem. 70:1976;241.
26. Ernster L., Zetterström R., Lindberg R. A method for the determination of tracer phosphate in biological material. Acta. Chem. Scand. 4:1950;942-947.
27. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-686.
28. Martin R.G., Ames B.C. A method for determining the sedimentation behavior of enzymes: application to protein mixture. J. Biol. Chem. 236:1961;1372-1379.
29. Vassault A. Lactate dehydrogenase: UV-method with piruvate and NADH: Methods of Enzymatic Analysis. In: Bergmeyer HU, editor. Weinheim: Basel, 1983;3:118-126.
30. Haga T., Haga K., Gilman A.G. Hydrodinamic properties of the β-adrenergic receptor and adenylate cyclase from wild type and variant S49 lymphoma cells. J. Biol. Chem. 252:1977;5776-5782.
31. Beattie B., Merrill A.R. In vitro enzyme activation and folded stability of Pseudomonas aeruginosa exotoxin A and its C-terminal peptide. Biochemistry. 35:1996;9042-9051.
32. Martínez-Carrion M., Critz W., Quashnock J. Molecular weight and subunits of serine transhydroxymethylase. Biochemistry. 11:1972;1613-1615.
33. Weber K., Osborn M.J. The reliability of molecular weight determination by dodecylsulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244:1969;4406-4412.
34. Ogawa H., Fujioka M. Purification and characterization of cytosolic and mitochondrial serine hydroxymethyltransferase from rat liver. J. Biochem. 90:1981;381-390.
35. Schirch L., Peterson D. Purification and properties of mitochondrial serine hydroxymethyltransferase. J. Biol. Chem. 250:1980;7801-7806.
36. Ulevitch R.J., Kallen R.G. Purification and characterization of pyridoxal-5′-phosphate dependent serine hydroxymethylase from lamb liver and its action upon β-phenylserines. Biochemistry. 16:1977;5342-5349.
37. Ramesh K., Rao N.A. Purification and physicochemical, kinetic and inmunological properties of allosteric serine hydroxymethyl transferase from monkey liver. Biochem. J. 187:1980;623-636.
38. Brahatheeswaran B., Prakash V., Savithri H., Rao N.A. Interaction of sheep liver apo-serine hydroxymethyltransferase with pyridoxal-5′-phosphate: a physicochemical, kinetic, and thermodynamic study. Arch. Biochem. Biophys. 330:1996;363-372.
39. Rao D.N., Rao N.A. Purification and regulatory properties of Mung Bean (Vigna radiata L.) serine hydroxymethyltransferase. Plant. Physiol. 69:1982;11-18.
40. Mitchell M., Reynolds P., Blevins D. Serine hydroxymethyltransferase from soybean root nodules: purification and kinetic properties. Plant. Physiol. 81:1986;553-557.
41. McNeil J.B., McIntosh E., Taylor B., Zhang F., Tang S., Bognar A. Cloning and molecular characterization of three genes, including two genes encoding serine hydroxymethyltransferases, whose inactivation is required to render yeast auxotrophic for glycine. J. Biol. Chem. 269:1994;9155-9165.
42. Kruschwitz H., McDonald D., Cossins E., Schirch V. Purification of Neurospora crassa cytosolic serine hydroxymethyltransferase. Adv. Exp. Med. Biol. 338:1993;719-722.
43. Miyasaki S., Toki S., Izumi Y., Yamada H. Purification and characterization of a serine hydroxymethyltransferase from an obligate methylotroph Hyphomicrobium methylovorum GM2. Eur. J. Biochem. 162:1987;533-540.
44. Zelikson R., Luzzati M. Two forms of serine transhydroxymethylase, one absent in a thymidylate-less mutant in Saccharomyces cerevisiae. Eur. J. Biochem. 64:1976;7-13.
45. Bello A., Nare B., Freedman D., Hardy L. PTR1: a reductase mediating salvage of oxidized pteridines and methotrexate resistance in the protozoan parasite Leishmania major. Proc. Nat. Acad. Sci. USA. 91:1994;11442-11446.
46. Wang J., Leblanc E., Cang C., Papadopoulou B., Bray T., Whiteley J.M., Lin S., Ouellette M. Pterine and folate reduction by the Leishmania tarentolae H locus short-chain dehydrogenase/reductase PTR1. Arch. Biochem. Biophys. 342:1997;197-202.
47. Wang M., Ratnam S., Freisheim J. Cloning nucleotide sequence and expression of the bifunctional dihydrofolate reductase-thymidylate synthase from Glycine max. Biochim. Biophys. Acta. 1261:1995;325-336.
48. Blakley RL. Folates and Pterines. In: Blakley RL, Benkovic SJ, editors. New York: Wiley-Interscience, 1984:1:191-253.
49. Hurt E., Pesold-Hurt B., Schatz G. The amino terminal region of an imported mitochondrion precursor polypeptide can direct cytoplasmic dihydrofolate reductase into the mitochondrial matrix. EMBO. J. 3:1984;3149-3156.
50. Neuburger M., Rébeille F., Jourdain A., Nakamura S., Douce R. Mitochondria are a major site for folate and thymidylate synthesis in plants. J. Biol. Chem. 271:1996;9466-9472.
51. Opperdoes F.R. The glycosome of trypanosomes and Leishmania. Biochem. Soc. Trans. 18:1990;729-731.