Cytoplasmic acidification is not an effector mechanism of VP16 or DEX-induced apoptosis in CEM T leukaemia cells

Journal of Cell Science

Volumn 112, Issue 11, 1999, Pages 1755-1760

  Benson, R S P ^a   Dive, C ^b   Watson, A J M ^a  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Acidification; Apoptosis; bcl 2; CEM cells; Death; Flow cytometry

Indexed keywords

DEXAMETHASONE; ETOPOSIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; PROTEIN BCL 2;

ACIDIFICATION; APOPTOSIS; ARTICLE; CELL PH; CHROMATIN CONDENSATION; CONTROLLED STUDY; CYTOPLASM; DRUG MECHANISM; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; PRIORITY JOURNAL; T CELL LEUKEMIA;

ACIDS; ANTINEOPLASTIC AGENTS, HORMONAL; APOPTOSIS; CYTOPLASM; DEXAMETHASONE; ETOPOSIDE; HUMANS; HYDROGEN-ION CONCENTRATION; NUCLEIC ACID SYNTHESIS INHIBITORS; PROTO-ONCOGENE PROTEINS C-BCL-2; TUMOR CELLS, CULTURED;

EID: 0033020267     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (39)

1. Antonsson, B., Conti, F., Ciavatta, A., Montessuit, S., Lewis, S., Martinou, I., Bernasconi, L., Bernard, A., Mermod, J. J., Mazzei, G., Maundrell, K., Gambale, F., Sadoul, R. and Martinou, J. C. (1997). Inhibition of Bax channel-forming activity by Bcl-2. Science 277, 370-372.
2. Arends, M. J. and Wyllie, A. H. (1991). Apoptosis: Mechanisms and roles in pathology. Int. Rev. Exp. Path. 32, 223-254.
3. 2+ and pH. Biochem. Biophys. Res. Commun. 186, 782-789.
4. Barry, M. A. and Eastman, A. (1993). Identification of deoxyribonuclease II as an endonuclease involved in apoptosis. Arch. Biochem. Biophys. 300, 440-450.
5. Benedetti, A., Di Sario, A., Svegliati Baroni, G. and Jezequel, A. M. (1995). Transforming growth factor beta 1 increases the number of apoptotic bodies and decreases intracellular pH in isolated periportal and perivenular rat hepatocytes. Hepatology 22, 1488-1498.
6. Benson, R. S. P., Heer, S., Dive, C. and Watson, A. W. (1996). Characterisation of cell volume loss in CEM-C7A cells during dexamethasone-induced apoptosis. Am. J. Physiol. 270, C1190-C1203.
7. Brunet, C. L., Gunby, R. H., Benson, R. S. P., Hickman, J. A., Watson, A. J. M. and Brady, G. (1997). Commitment to clonogenic BCL-2-regulated cell death in a human lymphoid cell line is unaffected by caspase inactivation. Cell Death Diff. 5, 107-115.
8. Chen, Q. C., Benson, R. S. R, Whetton, A. D., Brant, S. R., Donowitz, M., Montrose, M. H., Dive, C. and Watson, A. J. M. (1997). Role of acid/base homeostasis in the suppression of apoptosis in haemopoietic cells by v-Ab1 protein tyrosine kinase. J. Cell Sci. 110, 379-387.
9. Cohen, G. M. (1997). Caspases: the executioners of apoptosis. Biochem. J. 326, 1-16.
10. Dai, H. Y., Tsao, N., Leung, W. C. and Lei, H. Y. (1998). Increase of intracellular pH in p53-dependent apoptosis of thymocytes induced by gamma radiation. Radiation Res. 150, 183-189.
11. Dive, C., Gregory, C. D., Phipps, D. J., Evans, D. L., Milner, A. E. and Wyllie, A. H. (1992). Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry. Biochim. Biophys. Acta 1133, 275-285.
12. Dubrez, L., Savoy, I., Hammon, A. and Solary, E. (1996). Pivotal role of a DEVD-sensitive step in etoposide-induced and Fas-mediated apoptotic pathways. EMBO J. 15, 5504-5512.
13. Furlong, I. J., Ascaso, R., Lopez Rivas, A. and Collins, M. K. (1997). Intracellular acidification induces apoptosis by stimulating ICE-like protease activity. J. Cell Sci. 110, 653-661.
14. Furuya, Y., Lundmo, P., Short, A. D., Gill, D. L. and Isaacs, J. T. (1994). The role of calcium, pH, and cell proliferation in the programmed (apoptotic) death of androgen-independent prostatic cancer cells induced by thapsigargin. Cancer Res. 54, 6167-6175.
15. Gottlieb, R. A., Giesing, H. A., Engler, R. L. and Babior, B. M. (1995). The acid deoxyribonuclease of neutrophils: A possible participant in apoptosis-associated genome destruction. Blood 86, 2414-2418.
16. Gottlieb, R. A., Nordberg, J., Skowronski, E. and Babior, B. M. (1996). Apoptosis induced in Jurkat cells by several agents is preceded by intracellular acidification. Proc. Nat. Acad. Sci. USA 93, 654-658.
17. Huang, Y., Scher, B. M. and Waxman, S. (1995). Combination cytotoxic-differentiation therapy-induced apoptosis correlates with decreased in intracellular pH and activation of an endonuclease. Mol. Cell. Diff. 3, 241-260.
18. Kluck, R. M., Chapman, D. E., Egan, M., McDougall, C. A., Harmon, B. V., Moss, D. J., Kerr, J. F. and Halliday, J. W. (1993). Spontaneous apoptosis in NS-1 myeloma cultures: effects of cell density, conditioned medium and acid pH. Immunobiology 188, 124-133.
19. Lau, K. R., Elliott, A. C. and Brown, P. D. (1989). Acetylcholine-induced intracellular acidosis in rabbit salivary gland acinar cells. Am. J. Physiol. 256, C288-C295.
20. Lee, H. S., Park, H. J., Lyons, J. C., Griffin, R. J., Auger, E. A. and Song, C. W. (1997). Radiation-induced apoptosis in different pH environments in vitro. Int. J. Radiation Oncol. Biol. Phys. 38, 1079-1087.
21. Li, J. and Eastman, A. (1995). Apoptosis in an interleukin-2-dependent cytotoxic T lymphocyte cell line is associated with intracellular acidification. J. Biol. Chem. 270, 3203-3211.
22. Matsubara, K., Kubota, M., Adachi, S., Kuwakado, K., Hirota, H., Wakazono, Y., Akiyama, Y. and Mikawa, H. (1994). Different mode of cell death induced by calcium ionophore in human leukemia cell lines: possible role of constitutive endonuclease. Exp. Cell. Res. 210, 19-25.
23. Meisenholder, G. W., Martin, S. J., Green, D. R., Nordberg, J., Babior, B. M. and Gottlieb, R. A. (1996). Events in apoptosis: acidification is downstream of protease activation and bcl-2 protection. J. Biol. Chem. 271, 16260-16262.
24. Minn, A. J., Velez, P., Schendel, S. L., Liang, H., Muchmore, S. W., Fesik, S. W., Fill, M. and Thompson, C. B. (1997). Bcl-x(L) forms an ion channel in synthetic lipid membranes. Nature 385, 353-357.
25. Ormerod, M. G., Sun, X. M., Snowden, R. T., Davies, R., Fearnhead, H. and Cohen, G. M. (1993). Increased membrane permeability of apoptotic thymocytes: A flow cytometric study. Cytometry 14, 595-602.
26. Pérez-Sala, D., Collado-Escobar, D. and Mollinedo, F. (1995). Intracellular alkalinization suppresses lovastatin-induced apoptosis in HL-60 cells through the inactivation of a pH-dependent endonuclease. J. Biol. Chem. 270, 6235-6242.
27. Rebollo, A., Gomez, J., Martinez de Aragon, A., Lastres, P., Silva, A. and Perez-Sala, D. (1995). Apoptosis induced by IL-2 withdrawal is associated with an intracellular acidification. Exp. Cell. Res. 218, 581-585.
28. Reynolds, J. E., Li, J. F., Craig, R. W. and Eastman, A. (1996a). Bcl-2 and Mcl-1 expression in Chinese hamster ovary cells inhibits intracellular acidification and apoptosis induced by staurosporine. Exp. Cell. Res. 225, 430-436.
29. Reynolds, J. E., Li, J. and Eastman, A. (1996b). Detection of apoptosis by flow cytometry of cells simultaneously stained for intracellular pH (carboxy SNARF-1) and membrane permeability (Hoechst 33342). Cytometry 25, 349-357.
30. Reynolds, J. E., Wolf, C. M. and Eastman, A. (1997). Intracellular acidification is associated with, but not required for caspase activation, DNA fragmentation or apoptosis. Int. J. Oncol. 11, 1241-1246.
31. 2+-dependent endonuclease from human spleen: purification, properties, and role in apoptosis. Biochemistry 32, 9129-9136.
32. Rottenberg, H. and Scarpa, A. (1974). Calcium uptake and membrane potential in mitochondria. Biochemistry 13, 4811-4817.
33. Schendel, S. L., Xie, Z. H., Montal, M. O., Matsuyama, S., Montal, M. and Reed, J. C. (1997). Channel formation by antiapoptotic protein Bcl-2. Proc. Nat. Acad. Sci. USA 94, 5113-5118.
34. Shiokawa, D., Ohyama, H., Yamada, T., Takahashi, K. and Tanuma, S. (1994). Identification of an endonuclease responsible for apoptosis in rat thymocytes. Eur. J. Biochem. 226, 23-30.
35. - exchanger in spontaneous thymocyte apoptosis. J. Immunol. 157, 1107-1116.
36. + from a polarized human intestinal cell line. Am. J. Physiol. 261, G229-G238.
37. Whyte, M. K. B., Hardwick, S. J., Meagher, L. C., Savill, J. S. and Haslett, C. (1993). Transient elevations of cytosolic free calcium retard subsequent apoptosis in neutrophils in vitro. J. Clin. Invest. 92, 446-455.
38. Wood, A. C., Waters, C. M., Garner, A. and Hickman, J. A. (1994). Changes in c-myc expression and the kinetics of dexamethasone-induced programmed cell death (apoptosis) in human lymphoid leukaemia cells. Brit. J. Can. 69, 663-669.
39. + antiport and intracellular pH in the regulation of HL-60 cell apoptosis. Biochim. Biophys. Acta 1269, 122-128.