In vitro exposure effects of cyclosporin A and bis(tri-n-butyltin)oxide on lymphocyte proliferation, cytokine (receptor) mRNA expression, and cell surface marker expression in rat thymocytes and splenocytes

Toxicology

Volumn 135, Issue 1, 1999, Pages 49-66

  Vandebriel, Rob J ^a   Spiekstra, Sander W ^a   Hudspith, Barry N ^b   Meredith, Clive ^b   Van Loveren, Henk ^a  

^a NATIONAL INSTITUTE FOR PUBLIC HEALTH AND THE ENVIRONMENT   (Netherlands)

^b BIBRA Intl Woodmansterne Rd S ^*  (United Kingdom)

Author keywords

Bis(tri n butyltin)oxide; Cyclosporin A; Dot blot hybridisation; FACS; Lymphocyte transformation test; RT PCR

Indexed keywords

BIS(TRIBUTYLTIN) OXIDE; CD4 ANTIGEN; CD8 ANTIGEN; CELL SURFACE MARKER; CYCLOSPORIN A; CYTOKINE; IMMUNOSUPPRESSIVE AGENT; MESSENGER RNA;

ANIMAL CELL; ARTICLE; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DOT HYBRIDIZATION; DRUG EFFECT; FEMALE; IMMUNOTOXICITY; LYMPHOCYTE PROLIFERATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPLEEN CELL; THYMOCYTE;

ANIMALS; ANTIGENS, CD; ANTIGENS, CD4; ANTIGENS, CD8; CELL DIVISION; CELL SURVIVAL; CYCLOSPORINE; CYTOKINES; DOSE-RESPONSE RELATIONSHIP, DRUG; FEMALE; GENE EXPRESSION; IMMUNOSUPPRESSIVE AGENTS; INTERFERON TYPE II; INTERLEUKIN-10; INTERLEUKIN-2; LYMPHOCYTE ACTIVATION; LYMPHOCYTES; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; RATS; RATS, WISTAR; RECEPTORS, INTERLEUKIN-2; RNA, MESSENGER; SPLEEN; THYMUS GLAND; TRIALKYLTIN COMPOUNDS;

EID: 0033016377     PISSN: 0300483X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-483X(99)00049-9     Document Type: Article

References (43)

1. Amasaki Y., Masuda E.S., Imamura R., Arai K.-I, Arai N. Distinct NFAT family proteins are involved in the nuclear NFAT-DNA binding complexes from human thymocyte subsets. J. Immunol. 160:1998;2324-2333.
2. fyn. Cell. 70:1992;751-763.
3. Aw T.Y., Nicotera P., Manzo L., Orrenius S. Tributyltin stimulates apoptosis in rat thymocytes. Arch. Biochem. Biophys. 283:1990;46-50.
4. Bressa G., Hinton R.H., Price S.C., Isbir M., Ahmed R.S., Grasso P. Immunotoxicity of tri-n-butyltin oxide (TBTO) and tri-n-butyltin chloride (TBTC) in the rat. J. Appl. Toxicol. 11:1991;397-402.
5. Cai W., Hu L., Foulkes J.G. Transcription-modulating drugs: mechanism and selectivity. Curr. Opin. Biotechnol. 7:1996;608-615.
6. Chow S.C., Orrenius S. Rapid cytoskeleton modification in thymocytes induced by the immunotoxicant tributyltin. Toxicol. Appl. Pharmacol. 127:1994;19-26.
7. 2+efflux. Arch. Biochem. Biophys. 298:1992;143-149.
8. Demaison C., Fiette L., Blanchetière V., Schimpl A., Thèze J., Froussard P. IL-2 receptor α-chain expression is independently regulated in primary and secondary lymphoid organs. J. Immunol. 161:1998;1977-1982.
9. De Waal E.J., Schuurman H.-J., Rademakers L.H.P.M., Van Loveren H., Vos J.G. The cortical epithelium of the rat thymus after in vivo exposure to bis(tri-n-butyltin)oxide (TBTO). An (immuno)histochemical and ultrastructural study. Arch. Toxicol. 67:1993;186-192.
10. De Waal E.J., Schuurman H.-J., Van Loveren H., Vos J.G. Differential effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin, bis(tri-n-butyltin)oxide and cyclosporine on thymus histophysiology. Crit. Rev. Toxicol. 27:1997;381-430.
11. Dijkema R., van der Meide P.H., Pouwels P.H., Caspers M., Dubbeld M., Schellekens H. Cloning and expression of the chromosomal immune interferon gene of the rat. EMBO J. 4:1985;761-767.
12. Dijkema R., van der Meide P.H., Dubbeld M., Caspers M., Wubben J., Schellekens H. Cloning, expression, and purification of rat IFN-γ Methods Enzymol. 119:1986;453-464.
13. 32P-based hybridizations. Anal. Biochem. 210:1993;235-244.
14. Fort Ph., Piechaczyk M., El Sabrouty S., Dani Ch., Jeanteur Ph., Blanchard J.M. Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family. Nucleic Acids Res. 13:1985;1431-1442.
15. Gennari A., Potters M., Seinen W., Pieters R. Organotin-induced apoptosis as observed in vitro is not relevant for induction of thymus atrophy at antiproliferative doses. Toxicol. Appl. Pharmacol. 147:1997;259-266.
16. - thoracic duct T cells. Biochem. Biophys. Res. Commun. 189:1992;1-7.
17. Han C.W., Imamura M., Hashino S., Zhu X., Tanaka J., Imai K., Matsudaira T., Asano S. Differential effects of the immunosuppressants cyclosporin A, FK506 and KM2210 on cytokine gene expression. Bone Marrow Transplant. 15:1995;733-739.
18. Harada Y., Watanabe S., Yssel H., Arai K.I. Factors affecting the cytokine production of human T cells stimulated by different modes of activation. J. Allergy Clin. Immunol. 98:1996;S161-S173.
19. Ho S., Clipstone N., Timmermann L., Northrop J., Graef I., Fiorentino D., Nourse J., Crabtree G.R. The mechanism of action of cyclosporin A and FK506. Clin. Immunol. Immunopathol. 80:1996;S40-S45.
20. Jansen J.G., Vrieling H., van Zeeland A.A., Mohn G.R. The gene encoding hypoxanthine-guanine phosphoribosyltransferase as target for mutational analysis: PCR cloning and sequencing of the cDNA from the rat. Mut. Res. 266:1992;105-116.
21. Krajnc E.I., Wester P.W., Loeber J.G., Van Leeuwen F.X.R., Vos J.G., Vaessen H.A.M.G., Van Der Heijden C.A. Toxicity of bis(tri-n-butyltin)oxide in the rat. I. Short-term effects on general parameters and on the endocrine and lymphoid systems. Toxicol. Appl. Pharmacol. 75:1984;363-386.
22. Lanzillo J.J. Chemiluminescent nucleic acid detection with digoxigenin-labeled probes: a model system with probes for angiotensin converting enzyme which detect less than one attomole of target DNA. Anal. Biochem. 194:1991;45-53.
23. Lion T., Haas O.A. Nonradioactive labeling of probe with digoxigenin by polymerase chain reaction. Anal. Biochem. 188:1990;335-337.
24. Luster M.I., Portier C., Pait D.G., White K.L., Gennings C., Munson A.E., Rosenthal G.J. Risk assessment in immunotoxicology. I. Sensitivity and predictability of immune tests. Fundam. Appl. Toxicol. 18:1992;200-210.
25. McKnight A.J., Mason D.W., Barclay A.N. Sequence of rat interleukin 2 and anomalous binding of a mouse interleukin 2 cDNA probe to rat MHC class II-associated invariant chain mRNA. Immunogenetics. 30:1989;145-147.
26. +T cells. Eur. J. Immunol. 21:1991;1187-1194.
27. Page T.H., Dallman M.J. Molecular cloning of cDNAs for the rat interleukin 2 receptor α and β chain genes: differentially regulated gene activity in response to mitogenic stimulation. Eur. J. Immunol. 21:1991;2133-2138.
28. Raffray M., Cohen G.M. Bis(tri-n-butyltin)oxide induces programmed cell death (apoptosis) in immature rat thymocytes. Arch. Toxicol. 65:1991;135-139.
29. Raffray M., McCarthy D., Snowden R.T., Cohen G.M. Apoptosis as a mechanism of tributyltin cytotoxicity to thymocytes: relationship of apoptotic markers to biochemical and cellular effects. Toxicol. Appl. Pharmacol. 119:1993;122-130.
30. Richter G., Blankenstein T., Diamantstein T. Evolutionary aspects, structure, and expression of the rat interleukin 4 gene. Cytokine. 2:1990;221-228.
31. Ryffel B., Willcocks J.L., Brooks N., Woerly G. Interleukin-2 receptor (CD25) upregulation on human T-lymphocytes: sensitivity to immunosuppressants is defined by the mode of T-lymphocyte activation. Immunopharmacology. 30:1995;199-207.
32. Schorle H., Holtschke T., Hünig T., Schimpl A., Horak I. Development and function of T cells in mice rendered interleukin-2 deficient by gene targeting. Nature. 352:1991;621-624.
33. Snoeij N.J., Van Iersel A.A.J., Penninks A.H., Seinen W. Triorganotin-induced cytotoxicity to rat thymus, bone marrow and red blood cells as determined by several in vitro assays. Toxicology. 39:1986;71-83.
34. Snoeij N.J., Punt P.M., Penninks A.H., Seinen W. Effects of tri-n-butyltin chloride on energy metabolism, macromolecular synthesis, precursor uptake, and cyclic AMP production in isolated rat thymocytes. Biochim. Biophys. Acta. 852:1986;234-243.
35. Snoeij N.J., Van Rooijen H.J.M., Penninks A.H., Seinen W. Effects of various inhibitors of oxidative phosphorylation on energy metabolism, macromolecular synthesis and cyclic AMP production in isolated rat thymocytes. A regulating role for the cellular energy state in macromolecular synthesis and cyclic AMP production. Biochim. Biophys. Acta. 852:1986;244-253.
36. Snoeij N.J., Penninks A.H., Seinen W. Dibutyltin and tributyltin compounds induce thymus atrophy in rats due to a selective action on thymic lymphoblasts. Int. J. Immunopharmacol. 10:1988;891-899.
37. fyn mutant mice display differential signaling in thymocytes and peripheral T cells. Cell. 70:1992;741-750.
38. Vandebriel R.J., Meredith C., Scott M.P., Roholl P.J.M., Van Loveren H. Effects of in vivo exposure to bis(tri-n-butyltin)oxide, hexachlorobenzene, and benzo(a)pyrene on cytokine (receptor) mRNA levels in cultured rat splenocytes and on IL-2 receptor protein levels. Toxicol. Appl. Pharmacol. 148:1998;126-136.
39. Vandebriel R.J., Van Loveren H., Meredith C. Altered cytokine (receptor) mRNA expression as a tool in immunotoxicology. Toxicology. 130:1998;43-67.
40. Vos J.G., De Klerk A., Krajnc E.I., Kruizinga W., Van Ommen B, Rozing J. Toxicity of bis(tri-n-butyltin)oxide in the rat. II. Suppression of thymus-dependent immune responses and of parameters of nonspecific resistance after short-term exposure. Toxicol. Appl. Pharmacol. 75:1984;387-408.
41. Vos J.G., Krajnc E.I., Wester P.W. Immunotoxicity of bis(tri-n-butyltin)oxide. Dean J. et al. Immunotoxicology and immunopharmacology. 1985;327-340 Raven Press, New York.
42. Vos J.G., De Klerk A., Krajnc E.I., Van Loveren H., Rozing J. Immunotoxicity of bis(tri-n-butyltin)oxide in the rat: effects on thymus-dependent immunity and on nonspecific resistance following long-term exposure in young versus aged rats. Toxicol. Appl. Pharmacol. 105:1990;144-155.
43. Willerford D.M., Chen J., Ferry J.A., Davidson L., Ma A., Alt F.W. Interleukin-2 receptor α chain regulates the size and content of the peripheral lymphoid compartment. Immunity. 3:1995;521-530.