Quantitation and reproducibility problems in reversed-phase and size- exclusion HPLC analyses of wheat proteins

Cereal Chemistry

Volumn 76, Issue 2, 1999, Pages 299-302

  Huebner, F R ^a   Bietz, J A ^a  

^a NATIONAL CENTER FOR AGRICULTURAL UTILIZATION RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEINS; SIZE EXCLUSION CHROMATOGRAPHY;

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; LOW FLOW; POTENTIAL PROBLEMS; PROCEDURAL ERRORS; RAPID IDENTIFICATION; REPRODUCIBILITIES; REVERSED PHASE; SIZE EXCLUSION; SMALL SAMPLES; WHEAT PROTEINS;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

VEGETABLE PROTEIN;

ACCURACY; ARTICLE; CEREAL; CULTIVAR; FOOD ANALYSIS; GEL PERMEATION CHROMATOGRAPHY; NONHUMAN; PROTEIN ANALYSIS; QUANTITATIVE ASSAY; REPRODUCIBILITY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; WHEAT;

TRITICUM AESTIVUM;

EID: 0033012034     PISSN: 00090352     EISSN: None     Source Type: Journal    
DOI: 10.1094/CCHEM.1999.76.2.299     Document Type: Article

References (15)

1. Bietz, J. A. 1983. Separation of cereal proteins by reversed-phase high-performance liquid chromatography. J. Chromatogr. 255:219-238.
2. Bietz, J. A. 1984. Analysis of wheat gluten proteins by high-performance liquid chromatography. I. Baker's Dig. 58(1):15-17, 20-21, 32.
3. Bietz, J. A. 1986. High-performance liquid chromatography of cereal proteins. Pages 105-170 in: Advances in Cereal Science and Technology, Vol. 8. Y. Pomeranz, ed. Am. Assoc. Cereal Chem.: St. Paul, MN.
4. Bietz, J. A., and Wall, J. S. 1973. Isolation and characterization of gliadin-like subunits from glutenin. Cereal Chem. 50:537-547.
5. Bietz, J. A., Burnouf, T., Cobb, L. A. and Wall, J. S. 1984. Gliadin analysis by reversed-phase high-performance liquid chromatography: Optimization of extraction conditions. Cereal Chem. 61:124-129.
6. Dolan, J. W. 1996. Injection loop adsorption. LC-GC 14:562-566.
7. Huebner, F. R., and Wall, J. S. 1974. Wheat glutenin subunits. I. Preparative separation by gel-filtration and ion-exchange chromatography. Cereal Chem. 51:228-240.
8. Huebner, F. R. and Wall, J. S. 1980. Wheat glutenin: Effect of dissociating agents on molecular weight and composition as determined by gel filtration chromatography. J. Agric. Food Chem. 28:433-438.
9. Huebner, F. R., Donaldson, G. L., and Wall, J. S. 1974. Wheat glutenin subunits. II. Compositional differences. Cereal Chem. 51:240-249.
10. Huebner, F. R., Nelsen, T. C., and Bietz, J. A. 1994. Quantitative differences among hard wheat proteins as related to environment, quality and class. Page 468 in: Gluten Proteins 1993. Assoc. Cereal Res.: Detmold, Germany.
11. Huebner, F. R., Bietz, J. A., Webb, B. D., and Juliano, B. O. 1990. Rice cultivar identification by high-performance liquid chromatography of endosperm proteins. Cereal Chem. 67:129-135.
12. Huebner, F. R., Nelsen, T. C., Chung, O. K., and Bietz, J. A. 1997. Protein distributions among hard red winter wheat varieties as related to environment and baking quality. Cereal Chem. 74:123-128.
13. Kruger, J. E., and Bietz, J. A., eds. 1994. High-Performance Liquid Chromatography of Cereal and Legume Proteins. Am. Assoc. Cereal Chem.: St. Paul, MN.
14. Marchylo, B. A. 1994. Practical considerations in the RP-HPLC analysis of cereal and legume proteins. Pages 15-50 in: High-Performance Liquid Chromatography of Cereal and Legume Proteins. J. E. Kruger and J. A. Bietz, eds. Am. Assoc. Cereal Chem.: St. Paul, MN.
15. Marchylo, B. A., and Kruger, J. E. 1988. The effect of injection volume on the quantitative analysis of wheat storage proteins by reversed-phase high-performance liquid chromatography. Cereal Chem. 65:192-197.