Efficient nuclear fish on paraffin-embedded tissue sections using microwave pretreatment

BioTechniques

Volumn 26, Issue 3, 1999, Pages 416-422

  Bull, John H ^a   Harnden, Patricia ^a,b  

^a ASTRAZENECA   (United Kingdom)

^b ST JAMES S UNIVERSITY HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DIGOXIGENIN;

ACCURACY; ANALYTIC METHOD; ARTICLE; ARTIFACT REDUCTION; CHROMOSOME ANALYSIS; CONTROLLED STUDY; DNA DETERMINATION; FLUORESCENCE IN SITU HYBRIDIZATION; GENE SEQUENCE; HUMAN; HUMAN TISSUE; MICROWAVE IRRADIATION; PROSTATE; PROTEIN DEGRADATION; REACTION TIME; RELIABILITY; TISSUE FIXATION; TISSUE SECTION;

EID: 0033007523     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99263bm09     Document Type: Article

References (8)

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3. Henke, R.P. and N. Ahyan. 1994. Enhancement of hybridization efficiency in interphase cytogenetics on paraffin-embedded tissue sections by microwave treatment. Anal. Cell. Pathol. 6:319-325.
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7. Sibony, M., F. Commo, P. Callard and J.M. Gasc. 1995. Methods in laboratory investigation - enhancement of mRNA in situ hybridization signal by microwave heating. Lab. Invest. 73:586-591.
8. Wilkens, L., R. Vonwasielewski, M. Werner, M. Nolte and A. Georgii. 1996. Microwave pretreatment improves RNA-ISH in various formal in-fixed tissues using a uniform protocol. Pathol. Res. Pract. 192:588-594.