Antigen restoration of MIB-1 immunoreactivity in breast cancer: Combined use of enzyme predigestion and low temperature for improved measurement of proliferation indexes

Journal of Histotechnology

Volumn 22, Issue 2, 1999, Pages 103-106

  Elias, Jules M ^a,b   Rosenberg, Brad ^a   Margiotta, Michele ^a   Kutcher, Camille ^a  

^a STONY BROOK UNIVERSITY   (United States)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

Immunohistochemistry; Prognostic markers; Routine fixation; Solid tumors

Indexed keywords

KI 67 ANTIGEN; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY KI 67;

ARTICLE; BREAST CANCER; CELL PROLIFERATION; CONTROLLED STUDY; COOLING; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MITOSIS INDEX; MITOSIS RATE; PROGNOSIS;

EID: 0033003326     PISSN: 01478885     EISSN: None     Source Type: Journal    
DOI: 10.1179/his.1999.22.2.103     Document Type: Article

References (23)

1. McDonnell TJ: Cell division versus cell death: a functional model of multistep neoplasia. Mol Carcinog 8:209-13, 1993
2. Weinberg DS: Proliferation indices in solid tumors. In Advances in Pathology and Laboratory Medicine, Vol 5, Weinstein RS, Graham AR, Anderson RE et al (eds). Mossy Year Book, 1992, pp 163-191
3. Elias JM: Cell proliferation indexes - a biomarker in solid tumors. Biotech Histochem 72(2):78, 1997
4. Gerdes J, Li L, Schleuter C et al: Immunohistochemical and molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by the monoclonal antibody Ki-67. Am J Pathol 1:138, 1991
5. Imamura H, Haga S, Shimizu T et al: MIB1-determined proliferative activity in intraductal components and prognosis of invasive ductal breast carcinoma. Int J Cancer Res 88:1017-1023, 1997
6. Gasparini G, Pozza F, Meli S et al: Breast cancer kinetics: immunohistochemical determination of growth fractions by monoclonal antibody Ki-67 and correlation with flow-cytometric S-phase and with some features of tumor aggressiveness. Anticancer Res 11:2015, 1991
7. Arber JM, Riggs MW, Arber DA: Correlation among MIB-1, paraffin section proliferation index in low stage breast carcinoma. App Immunohistochem 5(2): 117, 1997
8. Layfield LJ, Saria EA, Berchuck A et al: Prognostic value of MIBl in advanced ovarian carcinoma as determined using automated immunohistochemistry and quantitative image analysis. J Surg Oncol 66:230-237, 1997
9. Forschen R, Kreigel A, Langen C et al: Assessment of proliferative activity in carcinomas of the human alimentary tract by Ki-67 immunostaining. Int J Cancer 47:686, 1991
10. Pich A, Ponti R, Valente G et al: MIB-1, Ki-67, and PCNA scores and DNA flow cytometry in intermediate grade malignant lymphoma. J Clin Pathol, 47:18, 1994
11. Holt PR, Moss SF, Kapetanakis AM et al: Is Ki-67 a better proliferative marker in the colon than proliferating nuclear antigen? Cancer Epidem Biomarkers Prevent 6:131-135, 1997
12. Hall PA, Levison DA, Woods AL et al: Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: An index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 162:285, 1990
13. Ellis PA, Makris A, Burton SA et al: Comparison of MIB-1 proliferation index with S-phase in human breast carcinomas. Brit J Cancer 73(5):640, 1996
14. Querzoli P, Albonico G, Ferretti S et al: MIB-1 proliferative activity in invasive breast cancer measured by image analysis. J Clin Pathol 49:926, 1996
15. Cattoretti G, Backer MHG, Key G et al: Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB-1 and MIB-3) detect proliferating cells in microwave-processed formalin fixed paraffin sections. J Pathol 168:357, 1992
16. Curran RC, Gregory J: The unmasking of antigen in paraffin sections of tissue by trypsin. Experientia 33:1400, 1977
17. Shi SR, Key ME, Kalra KL: Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue. J. Histochem Cytochem 39:3057, 1991
18. Shi S-R, Cote RJ, Young LL, Taylor CR: Antigen retrieval immunohistochemistry: Practice and development. J Histotechnol 20:145, 1997
19. Elias JM, Margiotta M: Low temperature antigen restoration of steroid hormone receptor proteins in routine paraffin sections. J Histotechnol 20(2): 149, 1997
20. Masood S: Standardization of immunobioassays as surrogate endpoints. J Cell Biochem (Suppl)19:28, 1994
21. Hitchkock CL: An exaltation of experts: Concerted efforts in the standardization of immunohistochemistry (editorial). Hum Pathol 25(1):2, 1994
22. Parrado C, Falkmer UG, Hoog G et al: A technique for automatic/interactive assessment of the proliferating fraction of neoplastic cells in solid tumors. A methodological study on the Ki-67 immunoreactive cells in human mammary carcinomas, including a comparison by means of DNA cytometry. Gen Diag 141(3-4):215, 1996
23. Hitchcock CL: Ki-67 staining as a means to simplify analysis of tumor cell proliferationl. Am J Clin Pathol 96:444-446, 1991