The block of Shaker K+ channels by κ-conotoxin PVIIA is state dependent

Journal of General Physiology

Volumn 114, Issue 1, 1999, Pages 125-140

  Terlau, Heinrich ^a   Boccaccio, Anna ^a   Olivera, Baldomero M ^b   Conti, Franco ^c  

^a MAX PLANCK INSTITUTE OF EXPERIMENTAL MEDICINE   (Germany)

^b UNIVERSITY OF UTAH   (United States)

^c CNR   (Italy)

Author keywords

Ion channel pore; Potassium binding site; Voltage gated potassium channels; Xenopus expression system

Indexed keywords

CONOTOXIN; ION CHANNEL; KAPPA CONOTOXIN PVIIA; POTASSIUM CHANNEL; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING SITE; ELECTROPHYSIOLOGY; MOLECULAR INTERACTION; NONHUMAN; POTASSIUM CURRENT; PROTEIN PROTEIN INTERACTION; RECEPTOR BLOCKING; RECEPTOR OCCUPANCY; SNAIL;

ANIMALS; CONOTOXINS; ELECTROPHYSIOLOGY; FEMALE; MOLLUSK VENOMS; OOCYTES; OSMOLAR CONCENTRATION; POTASSIUM; POTASSIUM CHANNEL BLOCKERS; POTASSIUM CHANNELS; SHAKER SUPERFAMILY OF POTASSIUM CHANNELS; XENOPUS LAEVIS;

EID: 0033003051     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.114.1.125     Document Type: Article

References (22)

1. +channels. Effects of channel gating, voltage, and ionic strength. J. Gen. Physiol. 91:317-333.
2. +conduction and selectivity. Science, 280:69-77.
3. Garcia, E., and D. Naranjo. 1999. Blocking mechanism of κ-PVIIA conotoxin on Shaker channels. Biophys. J. 76:A332. (Abstr.)
4. + channel. Biophys. J. 65:1613-1619.
5. + channel: peptide and channel residues mediating molecular recognition. Neuron. 12:1377-1388.
6. Hamill, O.P., A. Marty, E. Neher, B. Sakmann, and F.J. Sigworth. 1981. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch. 391:85-100.
7. Hille, B. 1992. Ionic channels of excitable membranes. 2nd ed. Sinauer Associates, Inc., Sunderland, MA. 362-422.
8. Hille, B., and W. Schwarz. 1978. Potassium channels as multi-ion single-file pores. J. Gen. Physiol. 72:409-442.
9. Hoshi, T., W. Zagotta, and R. Aldrich. 1990. Biophysical and molecular mechanisms of Shaker potassium channel inactivation. Science. 250:533-538.
10. Kamb, A., J. Tseng-Crank, and M. Tanouye. 1988. Multiple products of the Drosophila Shaker gene may contribute to potassium channel diversity. Neuron. 1:421-430.
11. Kim, M., D.J. Baro, C.C. Lanning, M. Doshi, J. Farnham, H.S. Moskowitz, J.H. Peck, B.M. Olivera, and R.M. Harris-Warrick. 1997. Alternative splicing in the pore-forming region of Shaker potassium channels. J. Neurosci. 17:8213-8224.
12. Krieg, P.A., and D.A. Melton. 1987. In-vitro RNA-synthesis with SP6 RNA-polymerase. Methods Enzymol. 155:397-441.
13. + channel. J. Gen. Physiol. 91:335-349.
14. + channel-blocking peptides. Neuron. 15:5-10.
15. Olivera, B.M., W.R. Gray, R. Zeikus, J.M. McIntosh, J. Varga, J. Rivier, V. de Santos, and L.J. Cruz. 1985. Peptide neurotoxins from fish-hunting cone snails. Science. 230:1338-1343.
16. Olivera, B.M., J. Rivier, C. Clark, C.A. Ramilo, G.P. Corpuz, F.C. Abogadie, E. Mena, S.R. Woodward, D.R. Hillyard, and L.J. Cruz. 1990. Diversity of conus neuropeptides. Science. 249:257-263.
17. Savarin, P., M. Guenneugues, B. Gilquin, H. Lamthanh, S. Gasparini, S. Zinn-Justin, and A. Mènez. 1998. Three-dimensional structure of κ-conotoxin PVIIA, a novel potassium channel-blocking toxin from cone snails. Biochemistry. 37:5407-5416.
18. Scanlon, M.J., D. Naranjo, L. Thomas, P.P. Alewood, R.J. Lewis, and D.J. Craik. 1997. Solution structure and proposed binding mechanism of a novel potassium channel toxin κ-conotoxin PVIIA. Structure (Lond.). 5:1585-1597.
19. +-channel. J. Biol. Chem. 77:1-6.
20. Stühmer, W. 1992. Electrophysiological recordings from Xenopus oocytes. Methods EnzymoL 207:319-339.
21. + channel through multiple binding sites. Neuron. 18:665-673.
22. Terlau, H., K. Shon, M. Grilley, M. Stocker, W. Stühmer, and B.M. Olivera. 1996. Strategy for rapid immobilization of prey by a fish-hunting marine snail. Nature. 381:148-151.