A multifrequency electron spin resonance study of T4 lysozyme dynamics

Biophysical Journal

Volumn 76, Issue 6, 1999, Pages 3298-3306

  Barnes, Jeff P ^a   Liang, Zhichun ^a   Mchaourab, Hassane S ^b   Freed, Jack H ^a,d   Hubbell, Wayne L ^c  

^a Department of Obstetrics Gynecology   (United States)

^b MEDICAL COLLEGE OF WISCONSIN   (United States)

^c JULES STEIN EYE INSTITUTE   (United States)

^d Department of Molecular Biology and Genetics   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LYSOZYME;

AQUEOUS SOLUTION; ARTICLE; CONTROLLED STUDY; DIFFUSION; DYNAMICS; ELECTRON SPIN RESONANCE; ENZYME ANALYSIS; MOTION; NONHUMAN; ROTATION;

EID: 0033000691     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(99)77482-5     Document Type: Article

References (26)

1. Artyukh, R. I., G. S. Kachalova, B. A. Samaryanov, and V. P. Timofeev. 1995. Dynamic mobility of the histidine-containing domain in spin-labeled lysozyme. Mol. Biol. 29:87-92.
2. Barnes, J. P., and J. H. Freed. 1997. Aqueous sample holders for high frequency electron spin resonance. Rev. Sci. Instrum. 68:2838-2846.
3. Berliner, L. J. 1998. Spin labeling: the next millenium. In Biological Magnetic Resonance, Vol. 14. L. J. Berliner, editor. Plenum, New York.
4. Brune, D., and S. Kim. 1993. Predicting protein diffusion coefficients. Proc. Natl. Acad. Sci. USA. 90:3835-3839.
5. Budil, D., K. Earle, and J. H. Freed. 1993. Full determination of the rotational diffusion tensor by electron paramagnetic resonance at 250 GHz. J. Phys. Chem. 97:1294-1303.
6. Budil, D. E., S. Lee, S. Saxena, and J. H. Freed. 1996. Nonlinear-least-squares analysis of slow-motional EPR spectra in one and two dimensions using a modified Levenberg-Marquardt algorithm. J. Mag. Res. A120:155-189.
7. Dalton, L. 1985. EPR and Advanced EPR Studies of Biological Systems. L. Dalton, editor, Ch. 1. CRC Press, Boca Raton, FL. 1-10.
8. + conduction and selectivity. Science. 280:69-77.
9. Earle, K., D. Budil, and J. H. Freed. 1993. 250-GHz EPR of nitroxides in the slow-motional regime: models of rotational diffusion. J. Phys. Chem. 97:13289-13297.
10. Earle, K., D. Budil, and J. H. Freed. 1996. Millimeter wave electron spin resonance using quasioptical techniques. In Advances in Magnetic and Optical Resonance. W. S. Warren, editor, Vol. 19. Academic Press, San Deigo. 253-321.
11. c. J. Chem. Phys. 106:9996-10015.
12. Gavish, B. 1986. Molecular dynamics and the transient strain model of enzyme catalysis. In The Fluctuating Enzyme. G. R. Welch, editor. Wiley-Interscience, New York.
13. Gibrat, J. F., and N. Go. 1990. Normal mode analysis of human lysozyme: study of the relative motion of the two domains and characterization of the harmonic motion. Proteins Struct. Funct. 8:258-279.
14. Hubbell, W. L., H. S. Mchaourab, C. Altenbach, and M. A. Lietzow. 1996. Watching proteins move using site-directed spin labeling. Structure. 4:779-783.
15. Krinichnyi, V. I. 1991. Investigation of biological systems by high resolution 2 mm wave band ESR. J. Biochem. Biophys. Methods. 23:1-30.
16. Lumry, R. 1995. The new paradigm for protein research. In Protein-Solvent Interactions. R. B. Gregory, editor. Dekker, New York. 1-136.
17. Mchaourab, H. S., M. A. Lietzow, K. Hideg, and W. L. Hubbell. 1996. Motion of spin-labeled side chains in T4 lysozyme. Correlation with protein structure and dynamics. Biochemistry. 35:7692-7704.
18. Miick, S. M., A. P. Todd, and G. L. Millhauser. 1991. Position-dependent local motions in spin-labeled analogues of a short a helical peptide determined by electron spin resonance. Biochemistry. 30:9498-9503.
19. Ondar, M. A., O. Ya. Grinberg, A. A. Dubinskii, and Ya. S. Lebedev. 1985a. Study of the effect of the medium on the magnetic resonance parameters of nitroxide radicals by high resolution EPR spectroscopy. Sov. J. Chem. Phys. 3:781-792.
20. Ondar, M. A., O. Ya. Grinberg, A. A. Dubinskii, A. F. Shestakov, and Ya. S. Lebedev. 1985b. ESR spectroscopy in the two millimeter band and magnetic resonance parameters. Sov. J. Chem. Phys. 2:83-92.
21. Pauling, L. 1948. Nature of forces between large molecules of biological interest. Nature. 161:707-709.
22. Polimeno, A., and J. H. Freed. 1995. Slow motional ESR in complex fluids: the slowly relaxing local structure model of solvent cage effects. J. Phys. Chem. 99:10995-11006.
23. Richard, L., L. Genberg, J. Deak, H. Chiu, and R. J. D. Miller. 1992. Picosecond phase grating spectroscopy of hemoglobin and myoglobin: energetics and dynamics of global protein motion. Biochemistry. 31: 10703-10715.
24. Smith, J., S. Cusack, B. Tidor, and M. Karplus. 1990. Inelastic neutron scattering analysis of low-frequency motions in proteins: harmonic and damped-harmonic models of bovine pancreatic trypsin inhibitor. J. Chem. Phys. 93:2974-2991.
25. Stryer, L. 1988. Mechanisms of Enzyme Action. In Biochemistry, 3rd Ed. W. H. Freeman, New York. 201-232.
26. Todd, A. P., and G. L. Millhauser. 1991. ESR spectra reflect local and global mobility in a short spin-labeled peptide throughout the α helix → coil transition. Biochemistry. 30:5515-5523.