Ty1-mediated integration of expression cassettes: Host strain effects, stability, and product synthesis

Biotechnology Progress

Volumn 12, Issue 4, 1996, Pages 548-554

  Lee, Frank W F ^a   Da Silva, Nancy A ^a  

^a University of California at Irvine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE;

ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; BIOSYNTHESIS; CHROMOSOME; CULTURE MEDIUM; GENE AMPLIFICATION; GENETICS; LACTOSE OPERON; METABOLISM; MOLECULAR CLONING; PLASMID; SACCHAROMYCES CEREVISIAE; SOUTHERN BLOTTING; TEMPERATURE; TRANSPOSON;

BETA-GALACTOSIDASE; BLOTTING, SOUTHERN; CHROMOSOMES, FUNGAL; CLONING, MOLECULAR; CULTURE MEDIA; DNA TRANSPOSABLE ELEMENTS; GENE AMPLIFICATION; GENES, BACTERIAL; GENOME, BACTERIAL; LAC OPERON; PLASMIDS; SACCHAROMYCES CEREVISIAE; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE;

EID: 0030199075     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp9600288     Document Type: Article

References (44)

1. Boeke, J. D. Transposable Elements in Saccharomyces cerevisiae. In Mobile DNA; Berg, D. E., Howe, M. M., Eds.; American Society of Microbiology: Washington, DC, 1989; pp 335-374.
2. Boeke, J. D.; Sandmeyer, S. B. Yeast Transposable Elements. In The Molecular and Cellular Biology of the Yeast Saccharomyces: Genome Dynamics, Protein Synthesis, and Energetics; Broach, J. R., Jones, E. W., Pringle, J., Eds.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1991; pp 193-261.
3. Boeke, J. D.; LaCroute, F.; Fink, G. R. A Positive Selection for Mutants Lacking Orotidine-5′-Phosphate Decarboxylase Activity in Yeast: 5-Fluoro-Orotic Acid Resistance. Mol. Gen. Genet. 1984, 197, 345-346.
4. Boeke, J. D.; Garfinkel, D. J.; Styles, C. A.; Fink, G. R. Ty Elements Transpose through an RNA Intermediate. Cell 1985, 40, 491-500.
5. Boeke, J. D.; Styles, C. A.; Fink, G. R. Saccharomyces cerevisiae SPT3 Gene Is Required for Transposition and Transpositional Recombination of Chromosomal Ty Elements. Mol. Cell. Biol. 1988, 6, 3575-3581.
6. Boeke, J. D.; Xu, H.; Fink, G. R. A General Method for the Chromosomal Amplification of Genes in Yeast. Science 1988, 239, 280-282.
7. Boeke, J. D.; Eichinger, D. J.; Natsoulis, G. Doubling Ty1 Element Copy Number in Saccharomyces cerevisiae: Host Genome Stability and Phenotypic Effects. Genetics 1991, 129, 1043-1052.
8. Cameron, R. R.; Loh, E. Y.; Davis, R. W. Evidence for Transposition of Dispersed Repetitive DNA Families in Yeast. Cell 1979, 16, 739-751.
9. Chalker, D. L.; Sandmeyer, S. B. Ty3 Integrates within the Region of RNA Polymerase III Transcription Initiation. Genes Dev. 1992, 6, 117-128.
10. Chisholm, G. E.; Genbauffe, F. S.; Cooper, T. G. Tau, a Repeated DNA Sequence in Yeast. Proc. Natl. Acad. Sci. U.S.A. 1984, 81, 2965-2969.
11. Clark, D. J.; Bilanchone, V. W.; Haywood, L. J.; Dildine, S. L.; Sandmeyer, S. B. A Yeast Sigma Composite Element, Ty3, Has Properties of a Retrotransposon. J. Biol. Chem. 1988, 263, 1413-1423.
12. Clarke, L.; Carbon, J. Isolation of a Yeast Centromere and Construction of Functional Small Circular Chromosomes. Nature (London) 1980, 257, 504-509.
13. Da Silva, N. A.; Bailey, J. E. Construction and Characterization of a Temperature-Sensitive Expression System in Recombinant Yeast. Biotechnol. Prog. 1989, 5, 18-26.
14. Durrin, L. K.; Mann, R. K.; Kayne, P. S.; Grunstein, M. Yeast Histone H4 N-Terminal Sequence Is Required for Promoter Activation In Vivo. Cell 1991, 65, 1023-1031.
15. Durrin, L. K.; Mann, R. K.; Grunstein, M. Nucleosome Loss Activates CUP1 and HIS3 Promoters to Fully Induced Levels in the Yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 1992, 12, 1621-1629.
16. Fujii, T.; Kondo, K.; Shimizu, F.; Sone, H.; Tanaka, J.-I.; Inoue, T. Application of a Ribosomal DNA Integration Vector in the Construction of a Brewer's Yeast Having α-Acetolactate Decarboxylase Activity. Appl. Environ. Microbiol. 1990, 56, 997-1003.
17. Garfinkel, D. J.; Boeke, J. D.; Fink, G. R. Ty Element Transposition: Reverse Transcriptase and Virus-Like Particles. Cell 1985, 42, 507-517.
18. Garfinkel, D. J.; Mastrangelo, M. F.; Sanders, N. J.; Shafer, B. K.; Strathern, J. N. Transposon Tagging Using Ty Elements in Yeast. Genetics 1988, 120, 95-108.
19. Gietz, R. D.; Sugino A. New Yeast-Escherichia coli Shuttle Vectors Constructed with In Vitro Mutagenized Yeast Genes Lacking Six-Base Pair Restriction Sites. Gene 1988, 74, 527-534.
20. Guarente, L.; Yocum, R. R.; Gifford, P. A GAL10-CYC1 Hybrid Yeast Promoter Identifies the GAL4 Regulatory Region as an Upstream Site. Proc. Natl. Acad. Sci. U.S.A. 1982, 79, 7410-7414.
21. Hansen, L. J.; Sandmeyer, S. B. Characterization of a Transpositionally Active Ty3 Element and Identification of the Ty3 Integrase Protein. J. Virol. 1990, 64, 2599-2607.
22. Hovland, P.; Flick, J.; Johnston, M.; Sclafani, R. A. Galactose as a Gratuitous Inducer of GAL Gene Expression in Yeasts Growing on Glucose. Gene 1989, 83, 57-64
23. Ito, H.; Fukuda, Y.; Murata, K.; Kimura, A. Transformation of Intact Yeast Cells Treated with Alkali Cations. J. Bacteriol. 1983, 753, 163-168.
24. Jacobs, E.; Dewerchin, M.; Boeke, J. D. Retrovirus-Like Vectors for Saccharomyces cerevisiae: Integration of Foreign Genes Controlled by Efficient Promoters into Yeast Chromosomal DNA. Gene 1988, 67, 259-269.
25. Ji, H.; Moore, D. P.; Blomberg, M. A.; Braiterman, L. T.; Voytas, D. F.; Natsoulis, G.; Boeke, J. D. Hotspots for Unselected Ty1 Transposition Events on Yeast Chromosome III Are near tRNA Genes and LTR Sequences. Cell 1993, 73, 1007-1018.
26. Jimenez, A.; Davies, J. Expression of a Transposable Antibiotic Resistance Element in Saccharomyces cerevisiae: a Potential Selection for Eukaryotic Cloning Vectors. Nature (London) 1980, 287, 869-871.
27. Joyce, C. M.; Grindley, N. D. F. Method of Determining whether a Gene of Escherichia coli Is Essential: Application to the polA Gene. J. Bacteriol. 1984, 158, 636-643.
28. Kingsman, A. J.; Gimlich, R. L.; Clarke, L.; Chinault, A. C.; Carbon, J. Sequence Variation in Dispersed Repetitive Sequences in Saccharomyces cerevisiae. J. Mol. Biol. 1981, 145, 619-632.
29. Kingsman, S. M.; Kingsman, A. J.; Dobson, M. J.; Mellor, J.; Roberts, N. A. Heterologous Gene Expression in Saccharomyces cerevisiae. Biotechnol. Genet. Eng. Rev. 1985, 3, 377-416.
30. Kirchner, J.; Sandmeyer, S. B. Proteolytic Processing of Ty3 Proteins Is Required for Transposition. J. Virol. 1993, 67, 19-28.
31. Lee, F. W. F.; Da Silva, N. A. Application of Ty1 for cloned gene insertion: amplification of a large regulated expression cassette in Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 1996, 44, 620-623.
32. Lopes, T. S.; Klootwijk, J.; Veenstra, A. E.; van der Aar, P. C.; van Heerikhuizen, H.; Raue, H. A.; Planta, R. J. High-Copy-Number Integration into the Ribosomal DNA of Saccharomyces cerevisiae: a New Vector for High-Level Expression. Gene 1989, 79, 199-206.
33. Napp, S. J.; Da Silva, N. A. Enhanced Productivity through Gratuitous Induction in Recombinant Yeast Fermentations. Biotechnol. Prog. 1994, 10, 125-128.
34. Paquin, C. E.; Williamson, V. M. Temperature Effects on the Rate of Ty Transposition. Science 1984, 226, 53-55.
35. Paquin, C. E.; Williamson, V. M. Ty Insertions at Two Loci Account for Most of the Spontaneous Antimycin A Resistance Mutations during Growth at 15 °C of Saccharomyces cerevisiae Strains Lacking ADH1. Mol. Cell. Biol. 1986, 6, 70-79.
36. Romanos, M. A.; Scorer C. A.; Clare J. J. Foreign Gene Expression in Yeast: a Review. Yeast 1992, 8, 423-488.
37. Sakai, A.; Shimizu, Y.; Hishinuma, F. Integration of Heterologous Genes into the Chromosome of Saccharomyces cerevisiae Using a Delta Sequence of Yeast retrotransposon Ty. Appl. Microbiol. Biotechnol. 1990, 33, 302-306.
38. Sambrook, J.; Fritsch, E. F.; Maniatis, T. Molecular Cloning: a Laboratory Manual, 2nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1989.
39. Sherman, F.; Fink, G. R.; Hicks, J. B. Methods in Yeast Genetics; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1986.
40. Srienc, F.; Campbell, J. L.; Bailey, J. E. Detection of Bacterial β-Galactosidase Activity in Individual Saccharomyces cerevisiae Cells by Flow Cytometry. Biotechnol. Lett. 1983, 5, 43-48.
41. Struhl, K.; Stinchcomb, D. T.; Scherer, S.; Davis, R. W. High Frequency Transformation of Yeast: Autonomously Replication of Hybrid DNA Molecules. Proc. Natl. Acad. Sci. U.S.A. 1979, 76, 1035-1039.
42. Voytas, D. F.; Boeke, J. D. Yeast Retrotransposon Revealed. Nature (London) 1992, 358, 717.
43. Wang, X.; Wang, Z.; Da Silva, N. A. G418 Selection and Stability of Cloned Genes Integrated at the Chromosomal δ Sequences of Saccharomyces cerevisiae. Biotechnol. Bioeng. 1996, 49, 45-51.
44. Xu, H.; Boeke, J. D. High Frequency Deletion between Homologous Sequences during Retrotransposition of Ty Elements in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 1987, 84, 8553-8557.