Screening procedure for Pichia pastoris clones containing multiple copy gene inserts

BioTechniques

Volumn 26, Issue 6, 1999, Pages 1042-1044

  Fairlie, W Douglas ^a   Russell, Patricia K ^a   Zhang, Hong Ping ^a   Breit, Samuel N ^a,b  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

^b ST VINCENT S HOSPITAL   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; ELECTROPORATION; GENE EXPRESSION REGULATION; GENE INSERTION; IMAGING SYSTEM; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS;

PICHIA PASTORIS;

EID: 0032996701     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99266bm06     Document Type: Article

References (6)

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2. Cregg, J.M., T.S. Vedvick and W.C. Raschke. 1993. Recent advances in the expression of foreign genes in Pichia pastoris. BioTechnology 11:905-909.
3. Hollenberg, C.P. and G. Gellisen. 1997. Production of proteins by methylotropic yeasts. Curr. Opin. Biotechnol. 8:554-560.
4. Kocken, C.H.M. and A.W. Thomas. 1996. Rapid screening and mapping of conformational epitopes expressed in the secretion expression system Pichia pastoris. Anal. Biochem. 239:111-112.
5. Scorer, C.A., J.J. Clare, W.R. McCombie, M.A. Romanos and K. Sreekrishna. 1994. Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression. BioTechnology 12:181-184.
6. Wung, J.L. and N.R.J. Gascoigne. 1996. Antibody screening for secreted proteins expressed in Pichia pastoris. BioTechniques 21:808-812.