Functional characterization of a human purine-selective, Na+-dependent nucleoside transporter (hSPNT1) in a mammalian expression system

Journal of Pharmacology and Experimental Therapeutics

Volumn 289, Issue 3, 1999, Pages 1487-1491

  Schaner, Marci E ^a   Wang, Juan ^a   Zhang, Lei ^a   Su, Sheng Fang ^b   Gerstin, Karin M ^a   Giacomini, Kathleen M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

2',3' DIDEOXYADENOSINE; ADENOSINE; CARRIER PROTEIN; CLADRIBINE; DIDANOSINE; INOSINE; URIDINE;

ARTICLE; GENE EXPRESSION; GENE EXPRESSION SYSTEM; HELA CELL; HUMAN; HUMAN CELL; INHIBITION KINETICS; MOLECULAR DYNAMICS; NUCLEOSIDE TRANSPORT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; SPECIES DIFFERENCE;

ANIMALS; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; DEOXYRIBONUCLEOSIDES; DIDEOXYNUCLEOSIDES; HELA CELLS; HUMANS; KIDNEY; KINETICS; MAMMALS; MEMBRANE TRANSPORT PROTEINS; RATS; RECOMBINANT PROTEINS; RIBONUCLEOSIDES; SUBSTRATE SPECIFICITY; TRANSFECTION;

EID: 0032994908     PISSN: 00223565     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Boleti H, Coe IR, Baldwin SA, Young JD and Caas CE (1997) Molecular identification of the equilibrative NBMPR-sensitive (es) nucleoside transporter and demonstration of an equilibrative NBMPR-insensitive (ei) transport activity in human erythroleukemia (K562) cells. Neuropharmacology 36:1167-1179.
2. Che M, Ortiz DF and Arias IM (1995) Primary structure and functional expression of a cDNA encoding the bile canalicular, purine-spednc Na(+)-nucleoside cotransporter. J Biol Chem 270:13596-13599.
3. Crawford CR and Belt JA (1991) Sodium-dependent, concentrative nucleoside transport in Walker 256 rat carcinosarcoma cells. Biochem Biophys Res Commun 175:846-851.
4. Crawford CR, Ng CY, Noel LD and Belt JA (1990) Nucleoside transport in L1210 murine leukemia cells. Evidence for three transporters. J Biol Chem 265:9732-9736.
5. Dahlig-Harley E, Eilam Y, Paterson AR and Cass CE (1981) Binding of nitrobenzylthioinosine to high-affinity sites on the nucleoside-transport mechanism of HeLa cells. Biochem J 200:295-305.
6. Fang X, Parkinson FE, Mowles DA, Young JD and Cass CE (1996) Functional characterization of a recombinant sodium-dependent nucleoside transporter with selectivity for pyrimidine nucleosides (cNT1rat) by transient expression in cultured mammalian cells. Biochem J 317:457-465.
7. Griffiths DA Hall SD and Sokol PP (1991) Interaction of 3′-azido-3′-deoxythymidine with organic ion transport in rat renal basolateral membrane vesicles. J Pharma-col Exp Ther 257:149-155.
8. Gutierrez MM and Giacomini KM (1993) Substrate selectivity, potential sensitivity and stoichiometry of Na(+)-nucleoside transport in brush border membrane vesicles from human kidney. Biochim Biophys Acta 1149:202-2088.
9. Gutierrez MM, Brett CM, Ott RJ, Hui AC and Giacomini KM (1992) Nucleoside transport in brush border membrane vesicles from human kidney. Biochim Biophys Acta 1108:1-9.
10. Huang QQ, Yao SY, Ritzel MW, Paterson AR, Cass CE and Young JD (1994) Cloning and functional expression of a complementary DNA encoding a mammalian nu-cleoside transport protein. J Biol Chem 269:17757-17760.
11. Kuttesch JF Jr and Nelson JA (1982) Renal handling of 2′-deoxyadenosine and adenosine in humans and mice. Cancer Chemother Pharmacol 8:221-229.
12. Nelson JA, Kuttesch JR Jr and Herbert BH (1983) Renal secretion of purine nucleosides and their analogs in mice. Biochem Pharmacol 32:2323-2327.
13. Plagemann PG (1991) Na(+)-dependent, concentrative nucleoside transport in rat macrophages. Specificity for natural nucleosides and nucleoside analogs, including dideoxynucleosides and comparison of nucleoside transport in rat, mouse and human macrophages. Biochem Pharmacol 42:247-252.
14. Pott C and Hiddemann W (1997) Purine analogs in the treatment of chronic lymphocytic leukemia. Leukemia 11(Suppl 2):S25-S28.
15. Ritzel MW, Yao SY, Huang MY, Elliott JF, Cass CE and Young JD (1997) Molecular cloning and functional expression of cDNAs encoding a human Na+-nucleoside cotransporter (hCNT1). Am J Physiol 272:C707-C714.
16. Saven A, Emanuele S, Kosty M, Koziol J, Ellison D and Piro L (1995) 2-Chlorode-oxyadenosine activity in patients with untreated, indolent non-Hodgkin's lymphoma. J Clin Oncol 13:570-574.
17. Saven A, Lee T, Kosty M and Piro L (1996) Cladribine and mitoxantrone dose escalation in indolent non-Hodgkin's lymphoma. J Clin Oncol 14:2139-2144.
18. Schaner ME, Wang J, Zevin S, Gerstin KM and Giacomini KM (1997) Transient expression of a purine-selective nucleoside transporter (SPNTint) in a human cell line (HeLa). Pharm Res (NY) 14:1316-1321.
19. Stine KC, Saylors RL, Williams LL and Becton DL (1997) 2-Chlorodeoxyadenosine (2-CDA) for the treatment of refractory or recurrent Langerhans cell histiocytosis (LCH) in pediatric patients. Med Pediatr Oncol 29:288-292.
20. Thorn JA and Jarvis SM (1996) Adenosine transporters. Gen Pharmacol 27:613-620.
21. Tobinai K, Ogura M, Hotta T, Kobayashi Y, Narabayashi M, Suzuki R, Kinoshita T, Kozuru M, Uike N and Ohashi Y (1997) Phase I study of cladribine (2-chlorodeoxyadenosine) in lymphoid malignancies. Cladribine Study Group. Jpn J Clin Oncol 27:146-153.
22. Wang J and Giacomini KM (1997) Molecular determinants of substrate selectivity in Na+-dependent nucleoside transporters. J Biol Chem 272:28845-18848.
23. Wang J, Su SF, Dresser MJ, Schaner ME, Washington CB and Giacomini KM (1997) Na(+)-dependent purine nucleoside transporter from human kidney: Cloning and functional characterization. Am J Physiol 273:F1058-F1065.