A high throughput assay of the hepatitis C virus nonstructural protein 3 serine proteinase

Journal of Virological Methods

Volumn 80, Issue 1, 1999, Pages 77-84

  Kakiuchi, Nobuko ^a   Nishikawa, Satoshi ^a   Hattori, Masao ^b   Shimotohno, Kunitada ^c  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b UNIVERSITY OF TOYAMA   (Japan)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

Flavonoids; Hepatitis C virus; High throughput assay; Proteinase inhibitor

Indexed keywords

3 HYDROXYFLAVONE; 6 HYDROXYFLAVONE; 7 HYDROXYFLAVONE; 7,8 DIHYDROXYFLAVONE; ACACETIN; APIGENIN; BAICALEIN; BIOCHANIN A; CAPILLARISIN; CHRYSIN; DAIDZEIN; FISETIN; FLAVANONE; FLAVONOID; GALANGIN; GENISTEIN; HESPERETIN; ISOFLAVONE DERIVATIVE; KAEMPFEROL; LINARIN; LUTEOLIN; MORIN; MYRICETIN; NARINGENIN; QUERCETIN; SERINE PROTEINASE; SERINE PROTEINASE INHIBITOR; UNCLASSIFIED DRUG; WOGONIN;

ARTICLE; DRUG SCREENING; ENZYME ASSAY; ENZYME INHIBITION; ENZYME KINETICS; FLUORESCENCE; HEPATITIS C VIRUS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VITRO STUDY; INTERMETHOD COMPARISON; PRIORITY JOURNAL; TECHNIQUE;

AMINO ACID SEQUENCE; HEPACIVIRUS; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; SERINE ENDOPEPTIDASES; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY; VIRAL NONSTRUCTURAL PROTEINS;

HEPATITIS C VIRUS;

EID: 0032993958     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(99)00034-8     Document Type: Article

References (31)

1. Bartenschlager R., Ahlborn-Laake L., Mous J., Jacobsen H. Nonstructural protein 3 of the hepatitis C virus encodes a serine-type proteinase required for cleavage at the NS3/4 and NS4/5 junctions. J. Virol. 67:1993;3835-3844.
2. Bernard F.X., Sable S., Cameron B. et al. Glycosylated flavones as selective inhibitors of topoisomerase IV. Antimicrob. Agents Chemother. 41:1997;992-998.
3. Bianchi E., Steinkuhler C., Taliani M., Urbani A., DeFrancesco R., Pessi A. Synthetic depsipeptide substrates for the assay of human hepatitis C virus protease. Anal. Biochem. 237:1996;239-244.
4. Bible K.C., Kaufmann S.H. Flavopiridol: a cytotoxic flavone that induces cell death in noncycling A549 human lung carcinoma cells. Cancer Res. 56:1996;4856-4861.
5. Carlson B.A., Dubay M.M., Sausville E.A., Brizuela L., Worland P.J. Flavopiridol induces G1 arrest with inhibition of cyclin-dependent kinase (CDK) 2 and CDK4 in humanbreast carcinoma cells. Cancer Res. 56:1996;2973-2978.
6. Choo Q.-L., Richman K.H., Han K.H. et al. Genetic organization and diversity of the hepatitis C virus. Proc. Natl. Acad. Sci. USA. 88:1991;2451-2455.
7. Critchfield J.W., Coligan J.E., Folks T.M., Butera S. Casein kinase II is a selective target of HIV-1 transcriptional inhibitors. Proc. Natl. Acad. Sci. USA. 94:1997;6110-6115.
8. Eckart M.R., Selby M., Masiarz F. et al. The hepatitis C virus encodes a serine protease involved in processing of the putative nonstructural proteins from the viral polyprotein precursor. Biochem. Biophys. Res. Commun. 192:1993;399-406.
9. Failla C., Tomei L., DeFrancesco R. Both NS3 and NS4A are required for proteolytic processing of hepatitis C virus nonstructural proteins. J. Virol. 68:1994;3753-3760.
10. Grakoui A., McCourt D.W., Wychowski C., Feinstone S.M., Rice C.M. Characterization of the hepatitis C virus-encoded serine proteinase: determination of proteinase-dependent polyprotein cleavage sites. J. Virol. 67:1993;2832-2843.
11. Hijikata M., Mizushima H., Akagi T. et al. Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus. J. Virol. 67:1993;4665-4675.
12. Hijikata M., Mizushima H., Tanji Y. et al. Proteolytic processing and membrane association of putative nonstructural proteins of hepatitis C virus. Proc. Natl. Acad. Sci. USA. 90:1993;19773-19777.
13. Houghton M., Weiner A, Han J., Kou G., Choo Q.-L. Molecular biology of the hepatitis C viruses: implications for diagnosis, development and control of viral disease. Hepatology. 14:1991;381-399.
14. Kakiuchi N., Hijikata M., Komoda Y., Tanji Y., Hirowatari Y., Shimotohno K. Bacterial expression and analysis of cleavage activity of HCV serine proteinase using recombinant and synthetic substrate. Biochem. Biophys. Res. Commun. 210:1995;1059-1065.
15. Kakiuchi N., Komoda Y., Hijikata M., Shimotohno K. Cleavage activity of hepatitis C virus serine proteinase. J. Biochem. 122:1997;749-755.
16. Kakiuchi N., Komoda Y., Komoda K., Okada S., Tani T., Shimotohno K. Non-peptide inhibitors of HCV serine proteinase. FEBS Lett. 421:1998;217-220.
17. Kato N., Hijikata M., Ootsuyama Y. et al. Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proc. Natl. Acad. Sci. USA. 87:1990;9524-9528.
18. Kim J.L., Morgenstern K.A., Lin C. et al. Crystal structure of the hepatitis C virus NS3 protease domain complexed with a synthetic NS4A cofactor peptide. Cell. 87:1996;343-355.
19. Komoda Y., Hijikata M., Tanji Y. et al. Processing of hepatitis C viral polyprotein in Escherichia coli. Gene. 145:1994;221-226.
20. Komoda Y., Hijikata M., Sato S., Asabe S., Kimura K., Simotohno K. Substrate requirements of hepatitis C virus serine proteinase for intermolecular polypeptide cleavage in Escherichia coli. J. Virol. 68:1994;7351-7357.
21. Lin C., Pragai B.M., Grakoui A., Xu J., Rice C.M. Hepatitis C virus NS3 serine proteinase: trans-cleavage requirements and processing kinetics. J. Virol. 68:1994;8147-8157.
22. Manabe S., Fuke I., Tanishita O. et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology. 19(8):1994;634-644.
23. Nagase H., Fields C.G., Fields G.B. Design and characterization of a fluorogenic substrate selectively hydrolyzed by stromelysin 1 (matrix metalloproteinase-3). J. Biol. Chem. 269:1994;20952-20957.
24. Steinkuhler C., Urbani A., Tomei L. et al. Activity of purified hepatitis C virus protease NS3 on peptide substrates. J. Virol. 70:1996;6694-6700.
25. Takamizawa A., Mori C., Fuke I. et al. Structure and organization of the hepatitis C virus genome isolated from human carriers. J. Virol. 65:1991;1105-1113.
26. Takeshita N., Kakiuchi N., Kanazawa T. et al. An enzyme-linked immunosorbent assay for detecting proteolytic activity of hepatitis C virus proteinase. Anal. Biochem. 247:1997;242-246.
27. Taliani M., Bianchi E., Narjes F. et al. A continuous assay of hepatitis C virus protease based on resonance energy transfer depsipeptide substrates. Anal. Biochem. 240:1996;60-67.
28. Tanji Y., Hijikata M., Hirowatari Y., Shimotohno K. Identification of the domain required for trans-cleavage activity of hepatitis C viral serine proteinase. Gene. 145:1994;215-219.
29. Tanji Y., Hijikata M., Satoh S., Kaneko T., Shimotohno K. Hepatitis C virus-encoded nonstructural protein NS4A has versatile functions in viral protein processing. J. Virol. 69:1995;1575-1581.
30. Tomei L., Failla C., Santolini E., DeFrancesco R., LaMonica L. NS3 is a serine protease required for processing of hepatitis C virus polyprotein. J. Virol. 67:1993;4017-4026.
31. Vishnuvardhan D., Kakiuchi N., Urvil P.T., Shimotohno K., Kumar P.K.R., Nishikawa S. Expression of highly active recombinant NS3 protease domain of hepatitis C virus in E. coli. FEBS Lett. 402:1997;209-212.