Production of secreted, soluble human two-domain CD4 protein in Escherichia coli

Journal of Immunological Methods

Volumn 224, Issue 1-2, 1999, Pages 19-24

  Osburne, Marcia S ^a   Neidhardt, Edith A ^a   Godoy, Jorge E ^a   Van Schravendijk, Marie R ^a   Grossman, Trudy H ^a  

^a Procept   (United States)

Author keywords

Bacterial expression; CD4; PelB leader sequence; Protein production; Uninduced expression

Indexed keywords

CD4 ANTIGEN;

ANIMAL CELL; ARTICLE; BINDING SITE; CONFORMATIONAL TRANSITION; ESCHERICHIA COLI; HELPER CELL; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN SECRETION; T LYMPHOCYTE ACTIVATION;

ANTIGENS, CD4; CELL EXTRACTS; ESCHERICHIA COLI; HUMANS; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0032984404     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00002-2     Document Type: Article

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