Protein extraction using sugar ester reverse micelles

Journal of Chemical Technology and Biotechnology

Volumn 74, Issue 3, 1999, Pages 221-226

  Naoe, Kazumitsu ^a   Nishino, Makiko ^a   Ohsa, Tomomi ^a   Kawagoe, Mikio ^a   Imai, Masanao ^b  

^a NARA NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

^b NIHON UNIVERSITY   (Japan)

Author keywords

Bioseparation; Extraction; Nonionic surfactant; Protein; Reverse micelle; Sugar ester

Indexed keywords

ADDITION REACTIONS; COMPOSITION EFFECTS; ESTERIFICATION; MICELLES; PROTEINS;

BIOSEPARATION; CRITICAL MICELLE CONCENTRATION (CMC); CYTOCHROME EXTRACTION; MAXIMUM FORWARD EXTRACTION; SUGAR ESTER REVERSE MICELLES;

SOLVENT EXTRACTION;

2 PROPANOL; BUFFER; CYTOCHROME C; ESTER; HEXANE; NONIONIC SURFACTANT; PROTEIN; SUGAR; TRIACYLGLYCEROL LIPASE;

CONFERENCE PAPER; ESTERIFICATION; EXTRACTION; MICELLE; PH; RHIZOPUS; SOLUBILIZATION;

EID: 0032982529     PISSN: 02682575     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4660(199903)74:3<221::AID-JCTB47>3.0.CO;2-O     Document Type: Conference Paper

References (20)

1. Brandani V, Giacomo GD and Spera L, Recovery of α-amylase extracted by reverse micelles. Process Biochem 31:125-128 (1996).
2. Chang Q-L and Chen J-Y, Liquid-liquid reversed micellar extraction for isolating enzymes, studies of the purification of α-amylase. Process Biochem 31:371-375 (1996).
3. Naoe K, Tamai M, Kawagoe M, Imai M and Shimizu M, Minimal AOT concentration of binary protein component in reverse micellar extraction. Tram IChemE Part C 75:143-147 (1997).
4. Göklen KE and Hatton TA, Liquid-liquid extraction of low molecular-weight proteins by selective solubilization in reversed micelles. Sep Sci Technol 22:831-841 (1987).
5. Lye GJ, Asenjo JA and Pyle DL, Reverse micellar mass-transfer processes, spray column extraction of lysozyme. AIChE J 42:713-726 (1996).
6. Ichikawa S, Imai M and Shimizu M, Solubilizing water involved in protein extraction using reversed micelles. Biotechnol Bioeng 39:20-26 (1992).
7. Kuboi R, Mori Y and Komasawa I, Determination and deactivation of porcine pancreas lipase in reverse micelle extraction (in Japanese). Kagaku Kogaku Ronbunshu 16:1060-1066 (1990).
8. Naoe K, Imai M and Shimizu M, Optimal amphiphile concentration for lysozyme extraction using reverse micelles, Trans IChemE Part C 74:163-170 (1996).
9. Ayala GA, Kamat S, Bechman EJ and Russell AJ, Protein extraction and activity in reverse micelles of a nonionic detergent. Biotechnol Bioeng 39:806-814 (1992).
10. Komives CF, Lilley E and Russell AJ, Biodegradation of pesticides in nonionic water-in-oil microemulsions of Tween 85, relationship between micelle structure and activity. Biotechnol Bioeng 43:946-959 (1994).
11. Vasudevan M, Tahan K and Wiencek JM, Surfactant structure effects in protein separations using nonionic microemulsions. Biotechnol Bioeng 46:99-108 (1995).
12. Ura O, Naoe K, Kawagoe M and Imai M, Extraction conditions for protein in Span 60 reverse micellar system, in Biochemical Engineering: Marching Toward the Century of Biotechnology, Ed by Shen ZY, Ouyang F, Yu JT and Cao ZA, Tsinghua Univ Press, Beijing. pp 942-945 (1997).
13. Nagayama K, Matsu-ura S, Doi T and Imai M, Kinetic characterization of esterification catalyzed by Rhizopus delemar lipase in lecithin - AOT microemulsion systems. J Mol Cat B : Enzymatic 4:25-32 (1998).
14. Kamiwatari F, Physical associative modification of protein by amphiphilic molecules (in Japanese), BS thesis, Tokyo University of Agriculture and Technology, Japan, (1990).
15. Komives CF, Osborne DE and Russell AJ, Characterization of a nonionic surfactant reversed micellar system for enzyme catalysis. J Phys Chem 98:369-376 (1994).
16. Pileni MP, Zemb T and Petit C, Solubilization by reverse micelles: solute localization and structure perturbation. Chem Phys Lett 118:414-420 (1985).
17. Peng Q and Luisi PL, The behavior of proteases in lecithin reverse micelles. Eur J Biochem 188:471-480 (1990).
18. Imai M, Natsume T, Naoe K, Shimizu M, Ichikawa S and Furusaki S, Hydrophilic surroundings requisite for the solubilization of proteins related with their hydrophobicity in the AOT reversed micellar extraction. Bioseparation 6:325-333 (1997).
19. Nagayama K, Imai M, Shimizu M and Doi T, Kinetic study of lipase-catalyzed hydrolysis in AOT and lecithin microemulsion systems (in Japanese). Seibutsu-kogaku 74:255-260 (1996).
20. Schmidli PK and Luisi PL, Lipase-catalyzed reactions in reverse micelles formed by soybean lecithin. Biocatalysis 3:367-376 (1990).