Transfectant influenza a viruses with long deletions in the NS1 protein grow efficiently in vero cells

Journal of Virology

Volumn 72, Issue 8, 1998, Pages 6437-6441

  Egorov, Andrej ^a,c   Brandt, Sabine ^a,b   Sereinig, Sabine ^a   Romanova, Julia ^a   Ferko, Boris ^a   Katinger, Dietmar ^a   Grassauer, Andreas ^a   Alexandrova, Galina ^c   Katinger, Hermann ^a   Muster, Thomas ^a,b  

^a UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

^b UNIVERSITY OF VIENNA   (Austria)

^c INSTITUTE OF EXPERIMENTAL MEDICINE   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS PROTEIN NS1;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; DELETION MUTANT; GENE DELETION; INFLUENZA VIRUS A; MOUSE; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION; TEMPERATURE SENSITIVE MUTANT; VERO CELL; VIROGENESIS;

EID: 0031925893     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.72.8.6437-6441.1998     Document Type: Article

References (33)

1. Baez, M., R. Taussig, J. J. Zazra, J. F. Young, P. Palese, A. Reisfeld, and A. M. Skalka. 1980. Complete nucleotide sequence of the influenza A/PR/8/34 virus NS gene and comparison with the NS genes of the A/Udorn/72 and A/FPV/Rostock/34 strains. Nucleic Acids Res. 8:5845-5858.
2. de la Luna, S., P. Fortes, A. Beloso, and J. Ortin. 1995. Influenza virus NS1 protein enhances the rate of translation initiation of viral mRNAs. J. Virol. 69:2427-2433.
3. Diaz, M. O., S. Ziemin, M. M. Le Beau, P. Pitha, S. D. Smith, R. R. Chilcote, and J. D. Rowley. 1988. Homozygous deletion of the alpha-and beta 1-interferon genes in human leukemia and derived cell lines. Proc. Natl. Acad. Sci. USA 85:5259-5263.
4. Egorov, A., L. M. Garmashova, I. V. Lukashok, G. N. Nevedomskaia, G. I. Aleksandrova, and A. I. Klimov. 1994. The NS gene - a possible determinant of apathogenicity of a cold-adapted donor of attenuation A/Leningrad/134/47/57 and its reassortants. Vopr. Virusol. 39:201-205. (In Russian.)
5. Enami, K., T. A. Sato, S. Nakada, and M. Enami. 1994. Influenza virus NS1 protein stimulates translation of the M1 protein. J. Virol. 68:1432-1437.
6. Enami, M., W. Luytjes, M. Krystal, and P. Palese. 1990. Introduction of site-specific mutations into the genome of influenza virus. Proc. Natl. Acad. Sci. USA 87:3802-3805.
7. Enami, M., and P. Palese. 1991. High-efficiency formation of influenza virus transfectants. J. Virol. 65:2711-2713.
8. Enami, M., G. Sharma, C. Benham, and P. Palese. 1991. An influenza virus containing nine different RNA segments. Virology 185:291-298. (Erratum, 186:798, 1992.)
9. Fortes, P., A. Beloso, and J. Ortin. 1994. Influenza virus NS1 protein inhibits pre-mRNA splicing and blocks mRNA nucleocytoplasmic transport. EMBO J. 13:704-712.
10. Greenspan, D., P. Palese, and M. Krystal. 1988. Two nuclear location signals in the influenza virus NS1 nonstructural protein. J. Virol. 62:3020-3026.
11. Hatada, E., and R. Fukuda. 1992. Binding of influenza A virus NS1 protein to dsRNA in vitro. J. Gen. Virol. 73:3325-3329.
12. Hatada, E., M. Hasegawa, K. Shimizu, M. Hatanaka, and R. Fukuda. 1990. Analysis of influenza A virus temperature-sensitive mutants with mutations in RNA segment 8. J. Gen. Virol. 71:1283-1292.
13. Hatada, E., S. Saito, N. Okishio, and R. Fukuda. 1997. Binding of the influenza virus NS1 protein to model genome RNAs. J. Gen. Virol. 78:1059-1063.
14. Krug, R. M., and P. R. Etkind. 1973. Cytoplasmic and nuclear virus-specific proteins in influenza virus-infected MDCK cells. Virology 56:334-348.
15. Krug, R. M., and R. Soeiro. 1975. Studies on the intranuclear localization of influenza virus-specific proteins. Virology 64:378-387.
16. Lazarowitz, S. G., R. W. Compans, and P. W. Choppin. 1971. Influenza virus structural and nonstructural proteins in infected cells and their plasma membranes. Virology 46:830-843.
17. Lu, Y., X. Y. Qian, and R. M. Krug. 1994. The influenza virus NS1 protein: a novel inhibitor of pre-mRNA splicing. Genes Dev. 8:1817-1828.
18. Lu, Y., M. Wambach, M. G. Katze, and R. M. Krug. 1995. Binding of the influenza virus NS1 protein to double-stranded RNA inhibits the activation of the protein kinase that phosphorylates the eIF-2 translation initiation factor. Virology 214:222-228.
19. Ludwig, S., U. Vogel, and C. Scholtissek. 1995. Amino acid replacements leading to temperature-sensitive defects of the NS1 protein of influenza A virus. Arch. Virol. 140:945-950.
20. Luytjes, W., M. Krystal, M. Enami, J. D. Pavin, and P. Palese. 1989. Amplification, expression, and packaging of foreign gene by influenza virus. Cell 59:1107-1113.
21. Marion, R. M., T. Aragon, A. Beloso, A. Nieto, and J. Ortin. 1997. The N-terminal half of the influenza virus NS1 protein is sufficient for nuclear retention of mRNA and enhancement of viral mRNA translation. Nucleic Acids Res. 25:4271-4277.
22. Marion, R. M., T. Zurcher, S. de la Luna, and J. Ortin. 1997. Influenza virus NS1 protein interacts with viral transcription-replication complexes in vivo. J. Gen. Virol. 78:2447-2451.
23. Nemeroff, M. E., W. Wang, Z. Chen, Y. Li, C. Chien, G. Montelione, J. Liu, P. Lynch, H. Berman, and R. M. Krug. 1997. Unique interactions of the influenza virus NS1 protein with host cell nuclear functions, abstr. 229, p. 164. In Abstracts of the 10th International Conference on Negative Strand Viruses, Dublin, Ireland.
24. Norton, G. P., T. Tanaka, K. Tobita, S. Nakada, D. A. Buonagurio, D. Greenspan, M. Krystal, and P. Palese. 1987. Infectious influenza A and B virus variants with long carboxyl terminal deletions in the NS1 polypeptides. Virology 156:204-213.
25. Ochman, H., A. S. Gerber, and D. L. Hartl. 1988. Genetic applications of an inverse polymerase chain reaction. Genetics 120:621-623.
26. Park, Y. W., and M. G. Katze. 1995. Translational control by influenza virus. Identification of cis-acting sequences and trans-acting factors which may regulate selective viral mRNA translation. J. Biol. Chem. 270:28433-28439.
27. Privalsky, M. L., and E. E. Penhoet. 1981. The structure and synthesis of influenza virus phosphoproteins. J. Biol. Chem. 256:5368-5376.
28. Qiu, Y., and R. M. Krug. 1994. The influenza virus NS1 protein is a poly(A)-binding protein that inhibits nuclear export of mRNAs containing poly(A). J. Virol. 68:2425-2432.
29. Qiu, Y., M. Nemeroff, and R. M. Krug. 1995. The influenza virus NS1 protein binds to a specific region in human U6 snRNA and inhibits U6-U2 and U6-U4 snRNA interactions during splicing. RNA 1:304-316.
30. Shaw, M. W., I. V. Kiseleva, A. Y. Egorov, M. L. Hemphill, and X. Xu. 1996. Nucleocapsid protein alone is sufficient for the generation of influenza transfectants, p. 433-436. In L. E. Brown, A. W. Hampson, and R. G. Webster (ed.), Options for the control of influenza III. Elsevier Science B.V., Amsterdam, The Netherlands.
31. Snyder, M. H., W. T. London, H. F. Maassab, R. M. Chanock, and B. R. Murphy. 1990. A 36 nucleotide deletion mutation in the coding region of the NS1 gene of an influenza A virus RNA segment 8 specifies a temperature-dependent host range phenotype. Virus Res. 15:69-83.
32. Treanor, J. J., R. Buja, and B. R. Murphy. 1991. Intragenic suppression of a deletion mutation of the nonstructural gene of an influenza A virus. J. Virol. 65:4204-4210.
33. Wolff, T., R. E. O'Neill, and P. Palese. 1996. Interaction cloning of NS1-I, a human protein that binds to the nonstructural NS1 proteins of influenza A and B viruses. J. Virol. 70:5363-5372.