Isolation and genetic determination of a Saccharomyces cerevisiae mutant that produces an endo-polygalacturonase

Journal of Bioscience and Bioengineering

Volumn 87, Issue 5, 1999, Pages 594-597

  Hirose, Aoto ^a   Fujii, Minako ^a   Kishida, Masao ^a   Kawasaki, Haruhiko ^a   Sakai, Takuo ^b  

^a Osaka City University   (Japan)

^b KINKI UNIVERSITY   (Japan)

Author keywords

Genetic determination; Mutant; Polygalacturonase; Repressor; Saccharomyces cerevisiae; Silent gene

Indexed keywords

POLYGALACTURONASE;

ARTICLE; GENE ISOLATION; GENETIC ANALYSIS; NONHUMAN; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; X CHROMOSOME;

SACCHAROMYCES CEREVISIAE;

EID: 0032976470     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(99)80120-0     Document Type: Article

References (17)

1. 1. Sakai, T., Sakamoto, T., Hallaert, J., and Vandamme, E. J.: Pectin, pectinase, and protopectinase: production, properties, and applications. Adv. Appl. Microbiol., 39, 213-294 (1993).
2. 2. Sakai, T.: Degradation of pectins, p. 57-81. In Winkelmann, G. (ed.), Microbial degradation of natural products. VHC Publishers, New York (1992).
3. 3. Sakai, T. and Okushima, M.: Purification and crystallization of a protopectin-solubilizing enzyme from Trichosporon penicillatum. Agric. Biol. Chem., 46, 667-676 (1978).
4. 4. Iguchi, K., Kishida, M., and Sakai, T.: Purification and characterization of three extracellular protopectinases with polygalacturonase activities from Trichosporon penicillatum. Biosci. Biotech. Biochem., 60, 603-607 (1996).
5. 5. Sakai, T., Okushima, M., and Yoshitake, S.: Purification, crystallization and some properties of endopolygalacturonase from Kluyveromyces fragilis. Agric. Biol. Chem., 48, 1951-1961 (1984).
6. 6. Yoshitake, S., Numata, T., Katsuragi, T., Hours, R. A., and Sakai, T.: Purification and characterization of pectin-releasing enzyme produced by Kluyveromyces wickerhamii. J. Ferment. Bioeng., 77, 370-375 (1994).
7. 7. Luh, B. S. and Phaff, H. J.: Studies on polygalacturonase of certain yeasts. Arch. Biochem. Biophys., 33, 212-227 (1951).
8. 8. Gainvors, A., Frezier, V., Lemaresquier, H., Lequart, C., Aigel, M., and Belarbi, A.: Detection of polygalacturonase, pectin-lyase and pectin-esterase activities in a Saccharomyces cerevisiae strain. Yeast, 10, 1311-1319 (1994).
9. 9. Hirose, N., Kishida, M., Kawasaki, H., and Sakai, T.: Molecular cloning and expression of a polygalacturonase gene in Saccharomyces cerevisiae. J. Ferment. Bioeng., 86, 332-334 (1998).
10. 10. Casadaban, M. J., Chou, J., and Cohen, S. N.: In vitro gene fusions that join an enzymatically active β-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J. Bacteriol., 143, 971-980 (1980).
11. 11. Starr, M. P., Chatterjee, A. K., Starr, P. B., and Buchanan, G. E.: Enzymatic degradation of polygalacturonic acid by Yersinia and Klebsiella species in relation to clinical laboratory procedures. J. Clin. Microbiol., 6, 379-386 (1977).
12. 12. Maniatis, T., Fritsch, E. F., and Sambrook, J.: Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1989).
13. 13. Somogyi, M.: Notes on sugar determination. J. Biol. Chem., 159, 19-23 (1952).
14. 14. Guarente, L.: Yeast promoters and lacZ fusions designed to study expression of cloned genes. Methods Enzymol., 101, 181-191 (1983).
15. 15. Spencer, J. F. T. and Spencer, D. M-: Yeast genetics, p. 65-106. In Campbell, I. and Duffus, J. H. (ed.), Yeast: a practical approach. IPL Press, Oxford (1988).
16. 16. Wach, A., Pick, H., and Philippsen, P.: Procedures for isolating yeast DNA for different purposes, p. 1-16. In Johnston, J. R. (ed.), Molecular genetics of yeast: a practical approach. IPL Press, Oxford (1994).
17. 17. Iguchi, K., Hirano, H., Kishida, M., Kawasaki, H., and Sakai, T.: Cloning of a protopectinase gene of Trichosporon penicillatum and its expression in Saccharomyces cerevisiae. Microbiology, 143, 1657-1664 (1997).