Purification and characterization of ACC oxidase and increase in its activity during ripening of pear fruit

Journal of the Japanese Society for Horticultural Science

Volumn 68, Issue 3, 1999, Pages 551-557

  Kato, Masaya ^a   Hyodo, Hiroshi ^a  

^a SHIZUOKA UNIVERSITY   (Japan)

Author keywords

ACC oxidase; Enzyme characterization; Ethylene; Fruit ripening; Pyrus communis

Indexed keywords

1 AMINOCYCLOPROPANE 1 CARBOXYLIC ACID OXIDASE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; RIPENING; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS;

BRASSICA OLERACEA VAR. BOTRYTIS; ESCHERICHIA COLI; PYRUS COMMUNIS;

EID: 0032969546     PISSN: 18823351     EISSN: None     Source Type: Journal    
DOI: 10.2503/jjshs.68.551     Document Type: Article

References (34)

1. Abeles, F. B., P. W. Morgan and M. E. Saltveit. 1992. Ethylene in plant biology. p. 26-55. Academic Press, San Diego.
2. Adams, D. O. and S. F. Yang. 1979. Ethylene biosynthesis: identification of 1-aminocyclopropane-1-carboxylic acid as an intermediate in the conversion of methionine to ethylene. Proc. Natl. Acad. Sci. USA 76: 170-174.
3. Barlow, J. N., Z. Zhang, P. John, J. E. Baldwin and C. J. Schofield. 1997. Inactivation of 1-aminocyclopropane -1-carboxylate oxidase involves oxidative modifications. Biochemistry 36: 3563-3569.
4. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
5. Dong, J. G., J. C. Fernández-Maculet and S. F. Yang. 1992. Purification and characterization of 1-aminocyclopropane-1-carboxylate oxidase from apple fruit. Proc. Natl. Acad. Sci. USA 89: 9789-9793.
6. Dupille, E., C. Rombaldi, J.-M. Lelièvre, J.-C. CLeyet-Marel, J.-C. Pech and A. Latché. 1993. Purification, properties and partial amino-acid sequence of 1-aminocyclopropane-1-carboxylic acid oxidase from apple fruits. Planta 190: 65-70.
7. Fernández-Maculet, J. C. and S. F. Yang. 1992. Extraction and partial characterization of the ethylene-forming enzyme from apple fruit. Plant Physiol. 99: 751-754.
8. Gerasopoulos, D. and D. G Richardson. 1997. Storage-temperature-dependent time separation of softening and chlorophyll loss from the autocatalytic ethylene pathway and other ripening events of 'Anjou' peais. J. Amer. Soc. Hort. Sci. 122: 680-685.
9. Hamilton, A. J., G. W. Lycett and D. Grierson. 1990. Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants. Nature 346: 284-287.
10. Hyodo, H., C. Hashimoto, S. Morozurni, W. Hu and K. Tanaka. 1993. Characterization and induction of the activity of 1-aminocyclopropane-1-carboxylate oxidase in the wounded mesocarp tissue of Cucurbita maxima. Plant Cell Physiol. 34: 667-671.
11. Kasai, Y., H. Hyodo, Y. Ikoma and M. Yano. 1998. Characterization of 1-aminocyclopropane-1-carboxylate (ACC) oxidase in broccoli florets and from Escherichia coli cells transformed with cDNA of broccoli ACC oxidase. Bot. Bull. Acad. Sin. 39: 225-230.
12. Knee, M. 1987. Development of ethylene biosynthesis in pear fruits at -1 °C . J. Exp. Bot. 38: 1724-1733.
13. Knee, M., N. E. Looney, S. G. S. Hatfield and S. M. Smith. 1983. Initiation of rapid ethylene synthesis by apple and pear fruits in relation to storage temperature. J. Exp. Bot. 34: 1207-1212.
14. Kuai, J. and D. R. Dilley. 1992. Extraction, partial purification and characterization of 1-aminocyclopropane-1 -carboxylic acid oxidase from apple fruit. Postharvest Biol. Technol. 1: 203-211.
15. Laemmili, U. K. 1970. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227: 680-685.
16. Lelièvre, J.-M., L. Tichit, L. Fillion, C. Larrigaudière, M. Vendrell and J.-C. Pech. 1995. Cold-induced accumulation of 1-aminocyclopropane-1-carboxylate oxidase protein in Granny Smith apples. Postharvest Biol. Technol. 5: 11-17.
17. Lelièvre, J.-M., L. Tichit, P. Dao, L. Fillion, Y.-W. Nam, J.-C. Pech and A. Latché. 1997. Effects of chilling on the expression of ethylene biosynthetic genes in passecrassane pear (Pyrus communis L.) fruits. Plant Mol. Biol. 33: 847-855.
18. Looney, N. E. 1972. Interaction of harvest maturity, cold storage, and two growth regulators on ripening of 'Bartlett' pears. J. Amer. Soc. Hort. Sci. 97: 81-83.
19. McGarvey, D. J. and R. E. Christoffersen. 1992. Characterization and kinetic parameters of ethylene-forming enzyme from avocado fruit. J. Biol.Chem. 267: 5964-5967.
20. Moya-Leon, M. A. and P. John. 1995. Purification and biochemical characterization of 1-aminocyclopropane-1-carboxylate oxidase from banana fruit. Phytochemistry 39: 15-20.
21. Murayama, H., D. Satoh, Y. Ohta and T. Fukushima. 1995. Effect of relative humidity on ripening of 'Le Lectier' pear fruit. Acta Hort. 398:187-193.
22. Nakatsuka, A., S. Shiomi, Y. Kubo and A. Inaba. 1997. Expression and internal feedback regulation of ACC synthase and ACC oxidase genes in ripening tomato fruit. Plant Cell Physiol. 38: 1103-1110.
23. Pogson, B. J., C. G. Downs and K. M. Davies. 1995. Differential expression of two 1-aminocyclopropane-1 -carboxylic acid oxidase genes in broccoli after harvest. Plant Physiol. 108: 651-657.
24. Prescott, A. G. and P. John. 1996. Dioxygenases: molecular structure and role in plant metabolism. Ann. Rev. Plant Physiol. Plant Mol. Biol. 47: 245-271.
25. Smith, J. J., P. Ververidis and P. John. 1992. Characterization of the ethylene-forming enzyme partially purified from melon. Phytochemistry 31:1485-1494.
26. Smith, J. J., Z. H. Zhang, C. J. Schofield, P. John and J. E. Baldwin. 1994. Inactivation of 1-aminocyclopropane-1-carboxylate (ACC) oxidase. J. Exp. Bot. 45: 521-527.
27. Ververidis, P. and P. John. 1991. Complete recovery in vitro of ethylene-forming enzyme activity. Phytochemistry 30: 725-727.
28. Vioque, B. and J. M. Castellano. 1994. Extraction and biochemical characterization of 1-aminocyclopropane-1-carboxylic acid oxidase from pear. Physiol. Plant. 90: 334-338.
29. Wang, C. Y., W. M. Mellenthin and E. Hansen. 1971. Effect of temperature on development of premature ripening in 'Bartlett' pears. J. Amer. Soc. Hort. Sci. 96: 122-125.
30. Wang, C. Y., W. M. Mellenthin and E. Hansen. 1972. Maturation of 'Anjou' pears in relation to chemical composition and reaction to ethylene. J. Amer. Soc. Hort. Sci. 97: 9-12.
31. Wang, C. Y., C. E. Sams and K. C. Gross. 1985. Ethylene, ACC, soluble polyuronide, and cell wall noncellulosic neutral sugar content in 'Eldorado' pears during cold storage and ripening. J. Amer. Soc. Hort. Sci. 110: 687-691.
32. Whittaker, D. J., G. S. Smith and R. C. Gardner. 1997. Expression of ethylene biosynthetic genes in Actinidia chinensis fruit. Plant Mol. Biol. 34:45-55.
33. Yamamoto, M., T. Miki., Y. Ishii, K. Fujinami, Y. Yanagisawa, H. Nakagawa, N. Ogura, T. Hirabayashi and T. Sato. 1995. The synthesis of ethylene in melon fruit during the early stage of ripening. Plant Cell Physiol. 36: 591-596.
34. Yang, S. F. and N. E. Hoffman. 1984. Ethylene biosynthesis and its regulation in higher plants. Ann. Rev. Plant Physiol. 35: 155-189.