Selectivity profile of muscarinic toxin 3 in functional assays of cloned and native receptors

Journal of Pharmacology and Experimental Therapeutics

Volumn 288, Issue 1, 1999, Pages 164-170

  Olianas, Maria C ^c   Ingianni, Angela ^c   Maullu, Carlo ^c   Adem, Abdu ^a   Karlsson, Evert ^a   Onali, Pierluigi ^b  

^a KAROLINSKA INSTITUTE   (Sweden)

^b UNIVERSITY OF CAGLIARI   (Italy)

^c NONE

Author keywords

[No Author keywords available]

Indexed keywords

CARBACHOL; HYPOPHYSIS ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE; METHYLSCOPOLAMINE; MUSCARINIC M1 RECEPTOR; MUSCARINIC M2 RECEPTOR; MUSCARINIC RECEPTOR; MUSCARINIC TOXIN 3; NEUROTOXIN; PHYSOSTIGMINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; INHIBITION KINETICS; NONHUMAN; PRIORITY JOURNAL; RECEPTOR AFFINITY; RECEPTOR BINDING; TOXIN ANALYSIS;

ADENYLATE CYCLASE; ANIMALS; CELL MEMBRANE; CHO CELLS; CRICETINAE; GENE EXPRESSION; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); HUMANS; MICE; MUSCARINIC ANTAGONISTS; N-METHYLSCOPOLAMINE; PEPTIDES; RECEPTOR, MUSCARINIC M1; RECEPTOR, MUSCARINIC M2; RECEPTOR, MUSCARINIC M4; RECEPTORS, MUSCARINIC; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SULFUR RADIOISOTOPES; TRITIUM; TUMOR CELLS, CULTURED;

EID: 0032962799     PISSN: 00223565     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (31)

1. 4 muscarinic acetylcholine receptors in the rat brain detected by a selective mamba toxin (MT3). Life Sci 56:1013.
2. Adern A and Karlsson E (1997) Muscarinic receptor subtype selective toxins. Life Sci 60:1069-1076.
3. 3H]pirenzepine binding to putative M1 muscarinic sites in the neuroblastoma x glioma hybrid cell line (NG108-15). Biochem Biophys Res Commun 119:289-297.
4. Arunlakshana O and Schild HO (1959) Some quantitative uses of drug antagonists. Br J Pharmacol 14:48-58.
5. Baumgold J and White T (1989) Pharmacological differences between muscarinic receptors coupled to phosphoinositide turnover and those coupled to adenylate cyclase inhibition. Biochem Pharmacol 38:1605-1616.
6. Bonner TI, Buckley NJ, Young AC and Brann MR (1987) Identification of a family of muscarinic acetylcholine receptor genes. Science 237:527-532
7. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.
8. Caufield MP (1993) Muscarinic receptors: Characterization, coupling and function. Pharmacol Ther 58:319-379.
9. 50) of an enzymatic reaction. Biochem Pharmacol 22:3099-3108.
10. Drescher DG, Upadhyay S, Wilcox E and Fex J (1992) Analysis of muscarinic receptor subtypes in the mouse cochlea by means of the polymerase chain reaction. J Neurochem 59:765-767.
11. Evans T, Smith MM, Tanner LI and Harden TK (1984) Muscarinic cholinergic receptors of two cell lines that regulate cyclic AMP metabolism by different molecular mechanisms. Mol Pharmacol 26:395-404.
12. Ferrari-Dileo G, Waelbroeck M, Mash DC and Flynn DD (1994) Selective labeling and localization of the M4 (m4) muscarinic receptor subtype. Mol Pharmacol 46:1028-1035.
13. + currents of muscarinic acetylcholine receptor subtypes in NG108-15 cells. Nature (Lond) 335:355-358.
14. Hulme EC, Birdsall NJM and Buckley NJ (1990) Muscarinic receptor subtypes. Annu Rev Pharmacol Toxicol 30:633-673.
15. Jolkkonen M, van Giersbergen PLM, Hellman U, Wernstedt C and Karlsson E (1994) A toxin from the green mamba Dendroaspis angusticeps: Amino acid sequence and selectivity for muscarinic m4 receptors. FEBS Lett 352:91-94.
16. Karlsson E, Jolkkonen M, Satypan N, Adem A, Kumlin E, Hellman U and Wernstedt C (1994) Protein toxins that bind to muscarinic acetylcholine receptors. Annu NY Acad Sci 710:153-161.
17. Lazareno S, Buckley NJ and Roberts FF (1990) Characterization of muscarinic M4 binding sites in rabbit lung, chicken heart, and NG108-15 cells. Mol Pharmacol 38:805-815.
18. 35S]GTPγS binding mediated by human muscarinic ml-m4 receptors: Antagonist studies. Br J Pharmacol 109:1120-1127.
19. Levey AI, Kitt CA, Simonds WF, Price DL and Brann MR (1991) Identification and localization of muscarinic acetylcholine receptor proteins in brain with subtype-specific antibodies. J Neurosci 11:3218-3226.
20. 2 muscarinic receptors using selective antagonists: Comparison with N1E-115 muscarinic receptors. J Pharmacol Exp Ther 257:1121-1129.
21. Michel AD, Delmendo R, Stefanich E and Whiting RL (1989) Binding characteristics of the muscarinic receptor subtype of NG108-15 cell line. Naunyn-Schmiedeberg's Arch Pharmakol 340:62-67.
22. Mieda M, Haga T and Saffen DW (1997) Expression of the rat m4 muscarinic acetylcholine receptor gene is regulated by the neuron-restrictive silencer element/ repressor element 1. J Biol Chem 272:5854-5860.
23. Munson PJ and Rodbard D (1980) LIGAND: A versatile computerized approach for characterization of ligand binding systems. Anal Biochem 107:220-239.
24. Olianas MC, Adem A, Karlsson E and Onali P (1996) Rat striatal muscarinic receptors coupled to the inhibition of adenylyl cyclase activity: Potent block by the selective m4 ligand muscarinic toxin 3 (MT3). Br J Pharmacol 118:283-288.
25. 35S]thiotriphosphate) binding by cholinergic muscarinic receptors in membranes of rat olfactory bulb. J Neurochem 67:2549-2556.
26. Peralta EG, Ashkenazi A, Winslow JW, Smith DH, Ramachandran J and Capon DJ (1987) Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors. EMBO J 6:3923-3929.
27. Peralta EG, Ashkenazi A, Winslow JW, Ramachandran J and Capon DJ (1988) Differential regulation of PI hydrolysis and adenylate cyclase by muscarinic receptor subtypes. Nature (Lond) 334:434-437.
28. Salomon Y, Londos D and Rodbell M (1974) A highly sensitive adenylate cyclase assay. Anal Biochem 58:541-548.
29. Tallarida RJ and Murray RB (1987) Manual of Pharmacologic Calculations with Computer Programs. Springler-Verlag, New York.
30. Wood IC, Roopra A and Buckley NJ (1996) Neural specific expression of the m4 muscarinic acetylcholine receptor gene is mediated by a RE1/NRSE-type silencing element. J Biol Chem 271:14221-14225.
31. Yasuda RP, Ciesla W, Flores L. R, Wall SJ, Li M, Satkus SA, Weisstein JS, Spagnola BV and Wolfe BB (1993) Development of antisera selective for m4 and m5 muscarinic cholinergic receptors: Distribution of m4 and m5 receptors in rat brain. Mol Pharmacol 43:149-157.