Rat striatal muscarinic receptors coupled to the inhibition of adenylyl cyclase activity: Potent block by the selective m4 ligand muscarinic toxin 3 (MT3)

British Journal of Pharmacology

Volumn 118, Issue 2, 1996, Pages 283-288

  Olianas, Maria C ^a   Adem, Abdu ^b   Karlsson, Evert ^b   Onali, Pierluigi ^a  

^a UNIVERSITY OF CAGLIARI   (Italy)

^b KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Adenylyl cyclase; Binding; Dendroaspis angusticeps toxin; Muscarinic receptor subtypes; Phosphoinositide hydrolysis; Rat cortex; Rat heart; Rat striatum; 3H N methylscopolamine

Indexed keywords

ACETYLCHOLINE; ADENYLATE CYCLASE; BRAIN RECEPTOR; DOPAMINE 1 RECEPTOR; FORSKOLIN; INOSITOL; INOSITOL PHOSPHATE; METHACHOLINE; METHYLSCOPOLAMINE; MUSCARINIC M4 RECEPTOR; MUSCARINIC RECEPTOR; MUSCARINIC RECEPTOR BLOCKING AGENT; NEUROTOXIN; SNAKE VENOM; TRITIUM;

ANIMAL CELL; ARTICLE; BINDING KINETICS; BINDING SITE; BRAIN CORTEX; CONTROLLED STUDY; CORPUS STRIATUM; ENZYME INHIBITION; HEART MUSCLE; INHIBITION KINETICS; MALE; NEUROTOXICITY; NONHUMAN; PRIORITY JOURNAL; RADIOASSAY; RAT;

EID: 0029922183     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1476-5381.1996.tb15400.x     Document Type: Article

References (28)

1. ADEM, A., ASBLOM, A., JOHANSSON, G., MBUGUA, P.M. & KARLSSON, E. (1988). Toxins from the venom of the green mamba Dendroaspis angusticeps that inhibit the binding of quinuclidinyl benzilate to muscarinic acetylcholine receptors. Biochim. Biophys. Acta, 968, 340-345.
2. 4 muscarinic cholinergic receptors in the rat brain detected by a selective mamba toxin (MT-3). Life Sci., 56, 1013.
3. ARUNLAKSHANA, O. & SCHILD, H.O. (1959). Some quantitative uses of drug antagonists. Br. J. Pharmacol. Chemother., 14, 48-57.
4. BRADFORD, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254.
5. BRANN, M.R., BUCKLEY, N.J. & BONNER, T.I. (1988). The Striatum and cerebral cortex express different muscarinic receptors mRNAs. FEBS Lett., 230, 90-94.
6. BUCKLEY, N.J., BONNER, T.I. & BRANN, M.R. (1988). Localization of a family of muscarinic receptor mRNAs in rat brain. J. Neurosci., 8, 4646-4652.
7. 3H] inositol labeling of phosphoinositides and agonist activation of phospholipase C in rat brain cortical membranes. J. Neurochem., 58, 2155-2161.
8. EHLERT, F.J., DELEN, F.M., YUN, S.H., FRIEDMAN, D.J. & SELF, D.W. (1989). Coupling of subtypes of the muscarinic receptor to adenylate cyclase in the corpus Striatum and heart. J. Pharmacol. Exp. Ther., 251, 660-671.
9. FORRAY, C. & EL-FAKAHANY, E. (1990). On the involvement of multiple muscarinic receptor subtypes in the activation of phosphoinositide metabolism in rat cerebral cortex. J. Pharmacol. Exp. Ther., 37, 893-902.
10. GIL, D.W. & WOLFE, B.B. (1985). Pirenzepine distinguishes between muscarinic receptors-mediated phosphoinositide breakdown and inhibition of adenylate cyclase. J. Pharmacol. Exp. Ther., 232, 608-616.
11. HERSCH, S.M., GUTEKUNST, C.-A., REES, H.D., HEILMAN, C.J. & LEVEY, A.I. (1994). Distribution of m1-m4 muscarinic receptor proteins in the rat striatum: Light and electron microscopic immunocytochemistry using subtype-specific antibodies. J. Neurosci., 14, 3351-3363.
12. JERUSALINSKI, D. & HARVEY, A.L. (1994). Toxins from mamba venoms: Small proteins with selectivities for different subtypes of muscarinic acetylcholine receptors. Trends Pharmacol. Sci., 15, 424-430.
13. JOLKKONEN, M., VAN GIERSBERGEN, P.L.M., HELLMAN, U., WERNSTEDT, C. & KARLSSON, E. (1994). A toxin from the green mamba Dendroaspis augusticeps: Amino acid sequence and selectivity for muscarinic m4 receptors. FEBS Lett., 352, 91-94.
14. KARLSSON, E., JOLKKONEN, M., SATYAPAN, N., ADEM, A., KUMLIN, E., HELLMAN, U. & WERNSTEDT, C. (1994). Protein toxins that bind to muscarinic acetylcholine receptors. Ann. N.Y. Acad. Sci., 710, 153-161.
15. KEEN, M. & NAHORSKI, S.R. (1988). Muscarinic acetylcholine receptors linked to the inhibition of adenylate cyclase activity in membranes from the rat striatum and myocardium can be distinguished on the basis of agonist efficacy. Mol. Pharmacol., 34, 769-778.
16. 4 binding sites in rabbit lung, chicken heart, and NG108-15 cells. Mol. Pharmacol., 38, 805-815.
17. MARTELL, A.E. & SMITH, R.M. (1975). Critical Stability Constants. pp. 269-284. New York: Plenum Press.
18. MAX, S.I., LIANG, J.-S. & POTTER, L.T. (1993a). Purification and properties of m1-toxin, a specific antagonist of m1 muscarinic receptors. J. Neurosci., 13, 4293-4300.
19. MAX, S.I., LIANG, J.-S., VALENTINE, H.H. & POTTER, L.T. (1993b). Use of m1-toxin as a selective antagonist of m1 muscarinic receptors. J. Pharmacol. Exp. Ther., 267, 480-485.
20. 2 receptor. J. Pharmacol. Exp. Ther., 250, 565-572.
21. MUNSON, P.J. & RODBARD, D. (1980). LIGAND: a versatile computerized approach for characterization of ligand-binding systems. Anal. Biochem., 107, 220-239.
22. OLIANAS, M.C. & ONALI, P. (1991). Muscarinic stimulation of adenylate cyclase activity of rat olfactory bulb. II. Characterization of the antagonist sensitivity and comparison with muscarinic inhibitions of the enzyme in striatum and heart. J. Pharmacol. Exp. Ther., 259, 680-686.
23. OLIANAS, M.C., ONALI, P., NEFF, N.H. & COSTA, E. (1983a). Adenylate cyclase activity of synaptic membranes from rat striatum. Inhibition by muscarinic receptor agonists. Mol. Pharmacol., 23, 393-398.
24. OLIANAS, M.C., ONALI, P., NEFF, N.H. & COSTA, E. (1983b). Muscarinic receptors modulate dopamine-activated adenylate cyclase of rat striatum. J. Neurochem., 41, 1364-1369.
25. ONALI, P., AASEN, A.J. & OLIANAS, M.C. (1994). Antagonism by (R)- and (S)-trihexyphenidyl of muscarinic stimulation of adenylyl cyclase in rat olfactory bulb and inhibition in striatum and heart. Br. J. Pharmacol., 113, 775-780.
26. PERALTA, E.G., ASHKENAZI, A., WINSLOW, J.W., RAMACHANDRAN, J. & CAPON, D.J. (1988). Differential regulation of PI hydrolysis and adenylyl cyclase by muscarinic receptor subtypes. Nature, 334, 434-437.
27. SALOMON, Y., LONDOS, D. & RODBELL, M. (1974). A highly sensitive adenylate cyclase assay. Anal. Biochem., 58, 541-548.
28. 4) in rat forebrain. Mol. Pharmacol., 38, 267-273.