Recombinant simian immunodeficiency virus expressing green fluorescent protein identifies infected cells in rhesus monkeys

AIDS Research and Human Retroviruses

Volumn 15, Issue 1, 1999, Pages 11-21

  Alexander, Louis ^a   Veazey, Ronald S ^a   Czajak, Susan ^a   DeMaria, Maryann ^a   Rosenzweig, Michael ^a   Lackner, Andrew A ^a   Desrosiers, Ronald C ^a   Sasseville, Vito G ^a,b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b BRISTOL MYERS SQUIBB   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; GENE INSERTION; GENE LOCUS; GENE SEQUENCE; IMMUNOPHENOTYPING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RHESUS MONKEY; SEQUENCE ANALYSIS; SIMIAN IMMUNODEFICIENCY VIRUS; VIRUS DETECTION; VIRUS GENOME; VIRUS RECOMBINANT; VIRUS REPLICATION;

ANIMALS; BASE SEQUENCE; CELL LINE; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; MACACA MULATTA; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; SIMIAN ACQUIRED IMMUNODEFICIENCY SYNDROME; SIMIAN IMMUNODEFICIENCY VIRUS; TRANSFECTION; VIRAL LOAD; VIRUS REPLICATION;

EID: 0032952599     PISSN: 08892229     EISSN: None     Source Type: Journal    
DOI: 10.1089/088922299311664     Document Type: Article

References (37)

1. Daniel MD, Letvin NL, King NW, Kannagi M, Sehgal PK, Hunt RD, Kanki PJ, Essex M, and Desroisiers RC: Isolation of T-cell tropic HTLV-III-like retrovirus from macaques. Science 1985; 228:1201-1204.
2. Letvin NL, Daniel MD, King NW, Kiyotaki M, Kannagi M, Chalifoux LV, Sehgal PK, Arthur LO, Allison AC, and Desrosiers RC: AIDS-like disease in macaque monkeys induced by simian immunodeficiency virus: A vaccine trial. In: Vaccines 87. Cold Spring Harbor Laboratory Press, Cold Springs Harbor, New York, 1987.
3. Baskin GB, Murphey-Corb M, Watson EA, and Martin LN: Necropsy findings in rhesus monkeys experimentally infected with cultured simian immunodeficiency virus (SIV)/delta. Vet Pathol 1988;25:456-467.
4. Gaines H, von Sydow M, Sönnerborg A, Albert J, Czajkowski J, Pehrson PO, Chiodi F, Moberg L, Fenyö EM, Asjö B, and Forsgren M: Antibody response in primary human immunodeficiency virus infection. Lancet 1987;i:1249-1253.
5. Cooper DA, Imrie AA, and Penny R: Antibody response to human immunodeficiency virus after primary infection. J Infect Dis 1987;155:1113-1118.
6. Gaines H, von Sydow M, Parry JV, Forsgren M, Pehrson PO, Sönnerborg A, Mortimer PP, and Strannegärd Ö: Detection of immunoglobulin M antibody in primary human immunodeficiency virus infection. AIDS 1988;2:11-15.
7. Lifson JD, Nowak MA, Goldstein S, Rossio JL, Kinter A, Vasquez G, Wiltrout TA, Brown C, Schneider D, Wahl L, Lloyd AL, Williams J, Elkins WR, Fauci AS, and Hirsch VM: The extent of early viral replication is a critical determinant of natural history of simian immunodeficiency virus infection. J Virol 1997;71: 9508-9514.
8. Spira AI, Marx PA, Patterson BK, Mahoney J, Koup RA, Wolinsky SM, and Ho DD: Cellular targets of infection and route of viral dissemination after an intravaginal inoculation of simian immunodeficiency virus into rhesus macaques. J Exp Med 1996; 183:215-225.
9. Veazey RS, DeMaria M, Chalifoux LV, Shvetz DE, Pauley DR, Knight HL, Rosenzweig M, Johnson RP, Desrosiers RC, and Lackner AA: Gastrointestinal tract as a major site of CD4+ T cell depletion and viral replication in SIV infection. Science 1998; 280:427-431.
10. Benson J, Chougnet C, Robert-Guroff M, Montefiori D, Markham P, Shearer G, Gallo RC, Cranage M, Paoletti E, Limbach K, Venzon D, Tartaglia J, and Franchini G: Recombinant vaccine-induced protection against the highly pathogenic simian immunodeficiency virus SIV(mac251): Dependence on route of challenge exposure. J Virol 1998;72:4170-4182.
11. Sasseville VG, Du Z, Chalifoux LV, Pauley DR, Young HL, Sehgal PK, Desrosiers RC, and Lackner AA: Induction of lymphocyte proliferation and severe gastrointestinal disease in macaques by a nef gene variant of SIVmac239. Am J Pathol 1996;149:163-176.
12. Lackner A, Vogel P, Ramos R, Kluge J, and Marthas M: Early events in tissues during infection with pathogenic (SIVmac239) and nonpathogenic (SIVmac1A11) molecular clones of SIV. Am J Pathol 1994;145:428-439.
13. Ho SN, Hunt HD, Horton RM, Pullen JK, and Pease LR: Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 1989;77:51-59.
14. Siemering K, Golbik R, Sever R, and Haseloff J: Mutations that suppress the thermosensitivity of green fluorescent protein. Curr Biol 1996;6:1653-1663.
15. Alexander L, Du Z, Rosenzweig M, Jung JU, and Desrosiers RC: A role for natural simian immunodeficiency virus and human immunodeficiency virus type 1 nef alleles in lymphocyte activation. J Virol 1997;71:6094-6099.
16. Kozak M: An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res 1987;15:8125-8132.
17. Shugars DC, Smith MS, Glueck DH, Nantermet PV, Seillier-Moiseiwitsch F, and Swanstrom R: Analysis of human immunodeficiency virus type 1 nef gene sequences present in vivo. J Virol 1993;67:4639-4650.
18. Regier DA and Desrosiers RC: The complete nucleotide sequence of a pathogenic molecular clone of simian immunodeficiency virus. AIDS Res Hum Retroviruses 1990;6:1221-1231.
19. Gibbs JS, Regier DA, and Desrosiers RC: Construction and in vitro properties of SIVmac mutants with deletions in the "nonessential" genes. AIDS Res Hum Retroviruses 1994;10:607-616.
20. Naidu YM, Kestler HW III, Li Y, Butler CV, Silva DP, Schmidt DK, Troup CD, Sehgal PK, Sonigo P, Daniel MD, and Desrosiers RC: Characterization of infectious molecular clones of simian immunodeficiency virus (SIVmac) and human immunodeficiency virus type 2: Persistent infection of rhesus monkeys with molecularly cloned SIVmac. J Virol 1988;62:4691-4696.
21. Pear WS, Nolan GP, Scott ML, and Baltimore D: Production of high-titer helper-free retroviruses by transient transfection. Proc Natl Acad Sci USA 1993;90:8392-8396.
22. Kawai T, Wong J, MacLean J, Cosimi AB, and Wee S: Characterization of a monoclonal antibody (6G12) recognizing the cynomolgus monkey CD3 antigen. Transplant Proc 1994;26:1845.
23. Kestler HW III, Ringler DJ, Mon K, Panicali DL, Sehgal PK, Daniel MD, and Desrosiers RC: Importance of the nef gene for maintenance of high virus loads and for the development of AIDS. Cell 1991;65:651-662.
24. Chalfie M, Tu Y, Euskirchen G, Ward WW, and Prasher DC: Green fluorescent protein as a marker for gene expression. Science 1994;263:802-805.
25. Jiang W and Hunter T: Analysis of cell-cycle profiles in transfected cells using a membrane-targeted GFP. BioTechniques 1997;24: 349-354.
26. Kaminchik J, Margalit R, Yaish S, Drummer H, Amit B, Sarver N, Gorecki M, and Panet A: Cellular distribution of HIV type 1 Nef protein: Identification of domains in Nef required for association with membrane and detergent-insoluble cellular matrix. AIDS Res Hum Retroviruses 1994;10:1003-1010.
27. Xiao CY, Hubner S, and Jans DA: SV40 large tumor antigen nuclear import is regulated by the double-stranded DNA-dependent protein kinase site (serine 120) flanking the nuclear localization sequence. J Biol Chem 1997;272:22191-22198.
28. Jang SK, Kräusslich H-G, Nicklin MJH, Duke GM, Palmenberg AC, and Wimmer E: A segment of the 5′ nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. J Virol 1988;62:2636-2643.
29. Gibbs JS, Lackner AA, Lang SM, Simon MA, Sehgal PK, Daniel MD, and Desrosiers RC: Progression to AIDS in the absence of genes for vpr or vpx. J Virol 1995;69:2378-2383.
30. Veazey RS, Rosenweig M, Shvetz DE, Pauley DR, DeMaria M, Chalifoux LV, Johnson RP, and Lackner AA: Characterization of gut-associated lymphoid tissues (GALT) of normal rhesus macaques. Clin Immunol Immunopathol 1997;82:230-242.
31. Kirchhoff F, Kestler HW III, and Desrosiers RC: Upstream U3 sequences in simian immunodeficiency virus are selectively deleted in vivo in the absence of an intact nef gene. J Virol 1994;68:2031-2037.
32. Schwartz S, Felber BK, and Pavlakis GN: Expression of human immunodeficiency virus type 1 vif and vpr mRNAs is Rev-dependent and regulated by splicing. Virology 1991;183:677-686.
33. Alexander L, Lee H, Rosenzweig M, Jung JU, and Desrosiers RC: An EGFP-containing vector system that facilitates stable and transient expression assays. Biotechniques 1997;23:64-66.
34. Sasseville VG, Rottman JB, Du Z, Veazey R, Knight HL, Caunt D, Desrosiers RC, and Lackner AA: Characterization of the cutaneous exanthem in macaques infected with a nef gene variant of SIVmac239. J Invest Dermatol 1998;110:894-901.
35. Aiken C, Konner J, Landau N, Lenburg ME, and Trono D: Nef induces CD4 endocytosis: Requirement for a critical dileucine motif in the membrane-proximal CD4 cytoplasmic domain. Cell 1994;76:853-864.
36. Chen M-Y, Maldarelli F, Karczewski MK, Willey RL, and Strebel K: Human immunodeficiency virus type 1 Vpu protein induces degradation of CD4 in vitro: The cytoplasmic domain of CD4 contributes to Vpu sensitivity. J Virol 1993;67:3877-3884.
37. Stevenson M, Meier C, Mann AM, Chapman N, and Wasiak A: Envelope glycoprotein of HIV induces interference and cytolysis resistance in CD4+ cells: Mechanism for persistence in AIDS. Cell 1988;53:483-496.