Improved recognition of MRSA case clusters by the application of molecular subtyping using pulsed-field gel electrophoresis

Journal of Hospital Infection

Volumn 41, Issue 1, 1999, Pages 29-37

  Macfarlane, L ^a   Walker, J ^a   Borrow, R ^a   Oppenheim, B A ^a   Fox, A J ^a  

^a WITHINGTON HOSPITAL   (United Kingdom)

Author keywords

Antibiogram; Methicillin resistant Staphylococcus aureus (MRSA); Phage typing; Pulsed field gel electrophoresis (PFGE)

Indexed keywords

ANTIBIOTIC AGENT; CIPROFLOXACIN; RIFAMPICIN;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL COLONIZATION; CONTROLLED STUDY; HOSPITAL INFECTION; INFECTION CONTROL; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR BIOLOGY; NONHUMAN; PULSED FIELD GEL ELECTROPHORESIS; RESOURCE MANAGEMENT; RETROSPECTIVE STUDY; STAPHYLOCOCCUS INFECTION; STRAIN DIFFERENCE;

EID: 0032949404     PISSN: 01956701     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0195-6701(99)90034-8     Document Type: Article

References (39)

1. Report of a combined Working Party of the Hospital Infection Society and British Society for Antimicrobial Chemotherapy. Revised guidelines for the control of epidemic methicillin-resistant Staphylococcus aureus. J Hosp Infect 1990; 16: 351-377.
2. Epidemic methicillin-resistant Staphylococcus aureus. Comm Dis Rep Weekly 1996; 23: 197.
3. Speller DCE, Johnson AP, James D, Marples RR, Charlett A, George RC. Resistance to methicillin and other antibiotics in isolates of Staphylococcus aureus from blood and cerebrospinal fluid, England and Wales, 1989-95. Lancet 1997; 350: 323-325.
4. Voss A, Milatovic D, Wallrauch-Schwarz C, Rosdahl VT, Braveny I. Methicillin-resistant Staphylococcus aureus in Europe. Eur J Clin Microbiol Infect Dis 1994; 13: 50-55.
5. Barrett SP, Teare EL, Sage R. Methicillin-resistant Staphylococcus aureus in three adjacent health districts of south-east England 1986-91. J Hosp Infect 1993; 24: 313-325.
6. Humphreys H, Duckworth G. Methicillin-resistant Staphylococcus aureus (MRSA) - a reappraisal of control measures in the light of changing circumstances. J Hosp Infect 1977; 36: 167-170.
7. Harstein AI. Improved understanding and control of nosocomial methicillin-resistant Staphylococcus aureus: are we overdoing it? Infect Control Hosp Epidemiol 1995; 16: 257-259.
8. Opal SM, Mayer KH, Stenberg MJ et al. Frequent acquisition of multiple strains of methicillin-resistant Staphylococcus aureus by healthcare workers in an endemic hospital environment. Infect Control Hosp Epidemiol 1990; 11: 479-485.
9. Hartstein AI, Denny MA, Morthland VH, LeMonte AM, Pfaller MA. Control of methicillin-resistant Staphylococcus aureus in a hospital and an intensive care unit. Infect Control Hosp Epidemiol 1995; 16: 405-411.
10. Layton MC, Hierholzer WJ, Patterson JE. The evolving epidemiology of methicillin-resistant Staphylococcus aureus at a university hospital. Infect Control Hosp Epidemiol 1995; 16: 12-17.
11. Rosenberg J. Methicillin-resistant Staphylococcus aureus (MRSA) in the community: who's watching? Lancet 1995; 346: 132-133.
12. Cox RA, Conquest C, Mallaghan C, Marples RR. A major outbreak of methicillin-resistant Staphylococcus aureus caused by a new phage-type (EMRSA-16). J Hosp Infect 1995; 29: 87-106.
13. Rodier L, de Wit D. MRSA colonization rates of readmitted patients previously colonized or infected with MRSA. J Hosp Infect 1997; 35: 161-163.
14. Bowler I. Strategies for the management of healthcare staff colonized with epidemic methicillin-resistant Staphylococcus aureus. J Hosp Infect 1997; 36: 321-322.
15. Sheppard MJ. Control of methicillin-resistant Staphylococcus aureus. J Hosp Infect 1996; 32: 73-75.
16. Cookson B, Peters B, Webster M, Phillips I, Rahman M, Noble W. Staff carriage of epidemic methicillin-resistant Staphylococcus aureus. J Clin Microbiol 1989; 27: 1471-1476.
17. Dawson SJ, Barnett J, Perry C, Jones EM, MacGowan AP, Reeves DS. Screening for EMRSA-16 in healthcare workers. J Hosp Infect 1997; 37: 75-77.
18. Lessing MPA, Jordens JZ, Bowler ICJ. When should healthcare workers be screened for methicillin-resistant Staphylococcus aureus? J Hosp Infect 1996; 34: 205-210.
19. Hancox R, Cummins A, Kelsey MC. An outbreak of EMRSA 2 associated with long-term colonization of medical staff, J Hosp Infect 1992; 22: 170-172.
20. Tenover FC, Arbeit R, Archer G et al. Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus. J Clin Microbiol 1994; 32: 407-415.
21. Bannerman TL, Hancock GA, Tenover FC, Miller JM. Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus. J Clin Microbiol 1995; 33: 551-555.
22. Khalifa KI, Heiba AA, Hancock G. Non typeable bacteriophage patterns of methicillin-resistant Staphylococcus aureus involved in a hospital outbreak. J Clin Microbiol 1989; 27: 2249-2251.
23. Cotter L, Lynch M, Cryan B, Greer P, Fanning S. Investigation of a methicillin-resistant Staphylococcus aureus (MRSA) outbreak in an Irish hospital: triplex PCR and DNA amplification fingerprinting. J Hosp Infect 1997; 36: 37-47.
24. Ichiyama S, Ohta M, Shimokata K, Kato N, Takeuchi J. Genomic DNA fingerprinting by pulsed-field gel electrophoresis as an epidemiological marker for study of nosocomial infections caused by methicillin-resistant Staphylococcus aureus. J Clin Microbiol 1991; 29: 2690-2695.
25. Lessing MPA, Jordens JZ, Bowler ICJ. Molecular epidemiology of a multiple strain outbreak of methicillin-resistant Staphylococcus aureus amongst patients and staff. J Hosp Infect 1995; 31: 253-260.
26. Prevost G, Pottcher B, Dahlet M, Bientz M, Mantz JM, Piemont Y. Pulsed-field gel electrophoresis as a new epidemiological tool for monitoring methicillin-resistant Staphylococcus aureus in an intensive care unit. J Hosp Infect 1991; 17: 255-269.
27. Trzcinski K, Hryniewicz W, Claus H, Witte W. Characterization of two different clusters of clonally related methicillin-resistant Staphylococcus aureus strains by conventional and molecular typing. J Hosp Infect 1994; 28: 113-126.
28. Struelens MJ, Deplano A, Godard C, Maes N, Serruys E. Epidemiologic typing and delineation of genetic relatedness of methicillin-resistant Staphylococcus aureus by macrorestriction analysis of genomic DNA by using pulsed-field gel electrophoresis. J Clin Microbiol 1992; 30: 2599-2605.
29. Tenover FC, Arbeit RD, Goering RV et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33: 2233-2239.
30. Arbeit RD. Laboratory procedures for the epidemiologic analysis of microorganisms. In: Murray PR, Baron EJ, Pfaller MA, Tenover EC, Yolken RH, Eds. Manual of Clinical Microbiology, 6th edn. Washington, DC: American Society for Microbiology 1995; 190-208.
31. Givney R, Vickery A, Holliday A, Pegler M, Benn R. Methicillin-resistant Staphylococcus aureus in a cystic fibrosis unit. J Hosp Infect 1997; 35: 27-36.
32. Hartstein AI, LeMonte AM, Iwamoto PKL. DNA typing and control of methicillin-resistant Staphylococcus aureus at two affiliated hospitals. Infect Control Hosp Epidemiol 1997; 18: 42-48.
33. Cookson BD, Aparicio P, Deplano A, Struelens M, Goering R, Marples R. Inter-centre comparison of pulsed-field gel electrophoresis for the typing of methicillin-resistant Staphylococcus aureus. J Med Microbiol 1996; 44 179-184.
34. Kreiswirth B, Kornblum J, Arbeit RD et al. Evidence for a clonal origin of methicillin resistance in Staphylococcus aureus. Science 1993; 259: 227-230.
35. Goering RV, Tenover FC. Epidemiological interpretation of chromosomal macro-restriction fragment patterns analysed by pulsed-field gel electrophoresis. J Clin Microbiol 1997; 35: 2432-2433.
36. Witte W, Cuny C, Zimmermann O et al. Stability of genomic DNA fragment patterns in methicillin-resistant Staphylococcus aureus (MRSA) during the course of intra- and inter-hospital spread. Eur J Epidemiol 1994; 10: 743-748.
37. Allen KD, Anson JJ, Parsons LA, Frost NG. Staff carriage of methicillin-resistant Staphylococcus aureus (EMRSA 15) and the home environment: a case report. J Hosp Infect 1997; 35: 307-311.
38. Masterton RG, Coia JE, Notman AW, Kempton-Smith L, Cookson BD. Refractory methicillin-resistant Staphylococcus aureus carriage associated with contamination of the home environment. J Hosp Infect 1995; 29: 318-319.
39. Corcoran GD. Carriage of methicillin-resistant Staphylococcus aureus and the home environment. J Hosp Infect 1997; 37: 74-75.