Investigation of a methicillin-resistant Staphylococcus aureus (MRSA) outbreak in an Irish hospital: Triplex PCR and DNA amplification fingerprinting

Journal of Hospital Infection

Volumn 36, Issue 1, 1997, Pages 37-47

  Cotter, L ^a   Lynch, M ^b   Cryan, B ^b   Greer, P ^c   Fanning, S ^a  

^a Munster Technological University   (Ireland)

^b University College Cork and INFANT Centre   (Ireland)

^c Regional Technical College   (Ireland)

Author keywords

DNA fingerprinting; Methicillin resistant Staphylococcus aureus (MRSA); Polymerase chain reaction (PCR); Triplex PCR

Indexed keywords

BACTERIAL DNA; DNA FRAGMENT;

ARTICLE; BACTERIAL GENE; COAGULASE NEGATIVE STAPHYLOCOCCUS; DNA FINGERPRINTING; IRELAND; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; POLYMERASE CHAIN REACTION;

EID: 0030910944     PISSN: 01956701     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0195-6701(97)90089-X     Document Type: Article

References (25)

1. Martin MA. Methicillin-resistant Staphylococcus aureus: the persistent resistant nosocomial pathogen. Curr Clin Top Infect Dis 1994; 14: 170-191.
2. Cookson B, Talsania H, Naidoo J, Phillips I. Strategies for typing and properties of epidemic methicillin-resistant Staphylococcus aureus. Eur J Clin Microbiol 1986; 5: 702-709.
3. Coia JE, Thompson-Carter F, Baird D, Platt DJ. Characterization of methicillin-resistant Staphylococcus aureus by biotyping, immunoblotting and restriction enzyme fragmentation patterns. J Med Microbiol 1990; 31: 125-132.
4. Prevost G, Jaulhac B, Piemont Y. DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates. J Clin Microbiol 1992; 4: 967-973.
5. van Belkum A. DNA fingerprinting of medically important microorganisms by use of PCR. J Clin Microbiol 1994; 7: 175-184.
6. Versalovic J, Koeuth T, Lupski JR. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acid Res 1991; 24: 6823-6831.
7. Hulton CSJ, Higgins CF, Sharp PM. ERIC sequences: a novel family of repetitive elements in the genomes of Escherichia coli, Salmonella typhimurium and other enterobacteria. Mol Microbiol 1991; 5: 825-834.
8. Martin B, Humbert O, Camara M, Guenzi E, Walker J, Mitchell T et al. A highly conserved repeated DNA element located in the chromosome of Streptococcus pneumoniae. Nucleic Acids Res 1992; 20: 3479-3483.
9. Versalovic J, Kapur V, Koeuth T, Mazurek GH, Whittman TS, Musser JM et al. DNA fingerprinting of pathogenic bacteria by fluorophore-enhanced repetitive sequence-based polymerase chain reaction. Arch Pathol Lab Med 1995; 119: 23-29.
10. Wenzel R, Herrmann R. Repetitive DNA in Mycoplasma pneumoniae. Nucleic Acids Res 1988; 16: 8337-8350.
11. Bignardi GE, Riley U, Easmon CSF. Use of the E test in methicillin sensitivity testing. J Antimicrob Chemother 1993; 32: 37-47.
12. Garrod LP, Lambert HP, O'Grady F. In: Antibiotics and Chemotherapy. 5th edn. Edinburgh: Churchill Livingstone. 1981.
13. Kizaki M, Kobayashi Y, Ikeda Y. Rapid and sensitive detection of the femA gene in staphylococci by enzymatic detection of polymerase chain reaction (ED-PCR): comparison with standard PCR analysis. J Hasp Infect 1994; 28: 287-295.
14. Brakstad OG, Aasbakk K, Maeland JA. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc Gene. J Clin Microbiol 1992; 7: 1654-1660.
15. Del Vecchio V, Petroziello JM, Gress MJ et al. Molecular genotyping of methicillin-resistant Staphylococcus aureus via fluorophore-enhanced repetitive-sequence PCR. J Clin Microbiol 1995; 33: 2141-2144.
16. Rogers DJ, Tanimoto TT. A computer program for classifying plants. Science 1960; 132: 1115-1118.
17. Spath H. Cluster Analysis Algorithms. Chichester: Ellis Horwood Ltd, 1982.
18. Bignardi GE, Woodford N, Chapman A, Johnson AP, Speller DCE. Detection of the mecA gene and phenotypic detection of resistance in Staphylococcus aureus isolates with borderline or low level methicilin resistance. J Antimicrob Chemother 1996; 37: 53-63.
19. de Lencastre H, Boudwijn L, de Jonge M, Mathews PR, Thomas A. Molecular aspects of methicillin resistance in Staphylococcus aureus. J Antimicrob Chemother 1994; 33: 7-24.
20. Servent AA, Bouziges N, Carles-Nurit MJ, Bourg G, Gouby A, Ramuz M. Use of low frequency-cleavage restriction endonucleases for DNA analysis in epidemiological investigations of nosocomial bacterial infections. J Clin Microbiol 1989; 27: 2057-2061.
21. Edwards MC, Gibbs RA. PCR: advantages, development and applications. PCR Meth Applic 1994; 3: S65-S75.
22. Sheehan C, Lynch M, Cullen C, Cryan B, Greer P, Fanning S. Genomic fingerprinting Acinetobacter baumanii amplification of multiple inter-repetitive extragenic palindromic sequences. J Hosp Infect 1995; 31: 33-40.
23. Parrish JE, Nelson DL. Practical aspects of fingerprinting human DNA using Alu PCR. Methods Mol Cell Biol 1994; 5: 71-77.
24. Stern MJ, Ferro-Luzzi Ames G, Smith NH, Robinson EC, Higgins CF. Repetitive extragenic palindromic sequences: a major component of the bacterial genome. Cell 1984; 37: 1015-1026.
25. Welsh J, McClelland M. Genomic fingerprints produced by PCR with consensus tRNA gene primers. Nucleic Acid Res 1991; 19: 861-866.