Determination of heparin-induced thrombocytopenia: A rapid flow cytometric assay for direct demonstration of antibody-mediated platelet activation

American Journal of Hematology

Volumn 61, Issue 1, 1999, Pages 53-61

  Tomer, Aaron ^a,c   Masalunga, Cynthia ^b   Abshire, Thomas C ^b  

^a BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

Author keywords

Flow cytometry; Heparin; Heparin induced thrombocytopenia (HIT); Thrombocytopenia; Thrombosis

Indexed keywords

DANAPAROID; ENOXAPARIN; HEPARIN;

ARTICLE; CASE REPORT; COMPLEX FORMATION; DRUG INDUCED DISEASE; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; FLOW CYTOMETRY; HEPARINIZATION; HUMAN; INFANT; PRIORITY JOURNAL; SEROTONIN RELEASE; THROMBOCYTE ACTIVATION; THROMBOCYTE AGGREGATION; THROMBOCYTE COUNT; THROMBOCYTOPENIA; THROMBOSIS;

EID: 0032946370     PISSN: 03618609     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1096-8652(199905)61:1<53::AID-AJH10>3.0.CO;2-F     Document Type: Article

References (29)

1. Sheridan D, Carter C, Kelton JG. A diagnostic test for heparin-induced thrombocytopenia. Blood 1986;67:27-30.
2. Kelton JG, Sheridan D, Santos A, Smith J, Steeves K, Smith C, Brown C, Murphy WG. Heparin-induced thrombocytopenia: laboratory studies. Blood 1988;72:925-930.
3. Chong BH. Heparin-induced thrombocytopenia. Blood Rev 1988;2(2): 108-114.
4. Alving B. Immune heparin-mediated thrombocytopenia and thrombosis. The Education Program of the American Society of Hematology. 1994. p 66-68.
5. Hirsh J, Raschke R, Warkentin TE, Dalen JE, Deykin D, Poller L. Heparin: mechanisms of action, pharmacokinetics, monitoring, efficacy, and safety. Chest 1995;108:258S-275S.
6. Visentin GP, Ford SE, Scott JP, Aster RH. Antibodies from patients with heparin-induced thrombocytopenia/thrombosis are specific for platelet factor 4 complexed with heparin or bound to endothelial cells. J Clin Invest 1994;93:81-88.
7. Aster RH. Heparin-induced thrombocytopenia and thrombosis [editorial]. N Engl J Med 1995;332:1374-1376.
8. Favoloro EJ, Bernal-Hoyos E, Exner T, Koutts J. Heparin-induced thrombocytopenia: laboratory investigation and confirmation of diagnosis. Pathology 1992;24:177-183.
9. Arepally G, Reynolds C, Tomaski A, Amiral J, Jawad A, Poncz M, Cines DB. Comparison of PF4/heparin ELISA assay with the 14C-serotonin release assay in the diagnosis of HIT. Am J Clin Pathol 1995;104:648-654.
10. Tomer A. A sensitive and specific functional flow cytometric assay for the diagnosis of heparin-induced thrombocytopenia. Br J Haematol 1997;98:648-656.
11. Tomer A, Stahl RL, Harker LA. Decreased platelet membrane glycoprotein (GP) IIb/IIIa receptor expression and activation in primary thrombocytosis. Blood 1990;76(Suppl 1):479a.
12. Tomer A, Eckman JR, Vroon DH. Activation of platelets and platelet procoagulant activity in sickle cell disease. Blood 1992;80(Suppl 1): 35.
13. Tomer A, Eckman JR, Scharf RE, Harker LA. In vivo platelet receptor activation and membrane procoagulant activity determined by flow cytometry. Thromb Haemost 1993;69:1531.
14. Tomer A, Stahl C, McClure H, Anderson D, Myers L, Liehl E, Winton EF. Effects of rhGMCSF on platelet survival and activation using a non-human primate model. Exp Hematol 1993;21:1577-1582.
15. Tomer A, Harker LA, Eckman JR. Prothrombotic state in sickle cell disease: Evidence for in vivo platelet and red cell procoagulant activity. Thromb Hemost 1995;73(6):956.
16. Scharf RE, Tomer A, Marzec UM, Teirstein PS, Ruggeri ZA, Harker LA. Activation of platelets in blood perfusing angioplasty-damaged coronary arteries: flow cytometric detection. Arterioscler Thromb 1992;12:1475-1481.
17. Harker LA, Hunt P, Kelly AB, Tomer A, Marzec UM, White DL, Wright J, Hanson SR, Stead RB. Regulation of platelet production and function by megakaryocyte growth and development factor in nonhuman primates. Blood 1996;87:1833-1844.
18. Chong BH, Murray B, Berndt MC, Dunlop LC, Brighton T, Chesterman CN. Plasma P-selectin is increased in thrombotic consumptive platelet disorders. Blood 1994;83:1535-1541.
19. Warkentin TE, Kelton JG. Interaction of heparin with platelets, including heparin-induced thrombocytopenia. In: Bounameaux H, editor (Goldhaber SZ, editor-in-chief). Low-molecular weight heparin in prophylaxis and therapy of thromboembolic diseases: fundamental and clinical cardiology. New York: Marcel Dekker; 1994. p75-127.
20. Warkentin TE, Hayward CPM, Boshkov LK, Santos AV, Sheppard JAI, Bode AP, Kelton JG. Sera from patients with heparin-induced thrombocytopenia generate platelet-derived microparticles with procoagulant activity: an explanation for the thrombotic complications of heparin-induced thrombocytopenia. Blood 1994;84:3691-699.
21. Colman RW, Cook JJ, Niewiarosi S. Mechanisms of platelet aggregation. In: Colman RW, Hirsh J, Marder VJ, Salzman EW, editors. Hemostasis and thrombosis. 3rd ed. Philadelphia: J.B. Lipincott; 1994;508-523.
22. Leroy J, Leclerc MH, Delahousse B, Guerois C, Feloppe P, Gruel Y, Toulemonde F. Treatment of heparin-associated thrombocytopenia and thrombosis with low-molecular weight heparin (CY 216). Sem Thromb Hemost 1985;11:326-329.
23. Kikta MJ, Keller MP, Mumphrey PW, Silver D. Can low-molecular weight heparin and heparinoids be safely given to patients with heparin-induced thrombocytopenia syndrome? Surgery 1993;114:705-710.
24. Boom DM, Kappers-Klunne MC, Michiels JJ, Stibbe J, van Vliet HH. Heparin-induced thrombocytopenia and thrombosis: a potential fatal complication in a routine treatment source. Netherl J Med 1995;46: 146-52.
25. Warkentin TE, Levin MN, Hirsh J, Horsewood P, Roberts RS, Tech M, Gent M, Kelton JG. Heparin-induced thrombocytopenia in patients treated with low-molecular heparin or unfractionated heparin. N Engl J Med 1995;332:1330-5.
26. Magnani HN. Heparin-induced thrombocytopenia (HIT): an overview of 230 patients treated with orgaran (Danaproid). Thromb Haemost 1993;70:554-61.
27. Greinacher A, Michels I, Mueller-Eckhardt C. Heparin-associated thrombocytopenia: The antibody is not heparin specific. Thromb and Hemost 1992;67:545-9.
28. Izban K, Leitz H, Blakemore C, Hoppensteadt D, Jeske W, Fareed J, Walenga JM. 14C-serotonin release assay in the laboratory diagnosis of heparin-induced thrombocytopenia. Blood 1997;90(suppl 1):461a.
29. Amiral J, Marfaing-Koka A, Wolf M, Alessi MD, Tardy B, Boyer-Neumann C, Vissac AM, Fresinud E, Poncz M, Meyer D. Presence of autoantibodies to interleukin-8 or neutrophil-activating peptide-2 in patients with heparin-associated thrombocytopenia. Blood 1996;88: 410-6.