Effects of mutations in proline 345 on insertion of diphtheria toxin into model membranes

Journal of Membrane Biology

Volumn 167, Issue 2, 1999, Pages 173-181

  Zhan, H ^a,c   Elliott, J L ^a   Shen, W H ^a,d   Huynh, P D ^b   Finkelstein, A ^b   Collier, R J ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^c Axys Pharmaceuticals Inc   (United States)

^d PFIZER INC   (United States)

Author keywords

Diphtheria toxin; Membrane insertion; Mutagenesis; Proline; Site specific labeling; Transmembrane domain

Indexed keywords

DIPHTHERIA TOXIN;

ARTICLE; CONFORMATIONAL TRANSITION; ENDOSOME; GENE INSERTION; NONHUMAN; POINT MUTATION; PROTEIN CONFORMATION; SITE DIRECTED MUTAGENESIS; VERO CELL;

ANIMALS; BIOLOGICAL TRANSPORT; CELL MEMBRANE; CERCOPITHECUS AETHIOPS; DIPHTHERIA TOXIN; LIPOSOMES; MUTATION; PROLINE; VERO CELLS;

BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 0032939820     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002329900481     Document Type: Article

References (34)

1. Ausubel, F., Brent, R. 1987. Current Protocols in Molecular Biology. John Wiley and Sons, New York
2. Barbieri, J.T., Collier, R.J. 1987. Expression of a mutant, full-length form of diphtheria toxin in Escherichia coli. Infect. Immun. 55:1647-1651
3. Bennett, M.J., Eisenberg, D. 1994. Refined structure of monomeric diphtheria toxin at 2.3 Å resolution. Protein Science 3:1464-1475
4. Choe, S., Bennett, M.J., Fujii, G., Curmi, P.M., Kanturdjieff, K.A., Collier, R.J., Eisenberg, D. 1992. The crystal structure of diphtheria toxin. Nature 357:216-22
5. Collins, C.M., Collier, R.J. 1987. Effects of acidic pH on the conformation and activity of diphtheria toxin. In: Membrane-mediated cytoxicity. B. Bonavida and R.J. Collier, editors, pp. 41-52. Alan R. Liss, New York
6. Consler, T.G., Tsolas, O., Kaback, H.R. 1991. Role of proline residues in the structure and function of a membrane transport protein. Biochemistry 30:1291-1297
7. DeLeers, M., Beugnier, N., Falmagne, P., Cabiaux, V., Ruysshcaert, J.M. 1983. Localization in diphtheria toxin fragment B of a region that induces pore formation in planar lipid bilayers at low pH. FEBS Lett. 160:82-86
8. Dunten, R.L., Sahin-Toth, M., Kaback, H.R. 1993. Cysteine scanning mutagenesis of putative helix XI in the lactose permease of Escherichia coli. Biochemistry 32:12644-12650
9. Falnes, P.Ø., Wiedlocha, A., Rapak, A., Olsnes, S. 1995. Farnesylation of CaaX-tagged diphtheria toxin A-fragment as a measure of transfer to the cytosol. Biochemistry 34:11152-11159
10. Greenfield, L., Bjorn, M.J., Horn, G., Fong, D., Buck, G.A., Collier, R.J., Kaplan, D.A. 1983. Nucleotide sequence of the structural gene for diphtheria toxin carried by corynebacteriophage beta. Proc. Natl. Acad. Sci. USA 80:6853-6857
11. Johnson, V.G., Nicholls, P.J., Habig, W.H., Youle, R.J. 1993. The role of proline 345 in diphtheria toxin translocation. J. Biol. Chem. 268:3514-3519
12. Kaul, P., Silverman, J., Shen, W.H., Blanke, S.R., Huynh, P.D., Finkelstein, A., Collier, R.J. 1996. Roles of Glu 349 and Asp 352 in membrane insertion and translocation by diphtheria toxin. Protein Sci. 5:687-692
13. Koehler, T.M., Collier, R.J. 1991. Anthrax toxin protective antigen: low-pH-indueed hydrophobicity and channel formation in liposomes. Mol. Microbiol. 5:1501-1506
14. Koradi, R., Billeter, M., Wuthrich, K. 1996. MOLMOL: a program for display and analysis of macromolecular structures. J. Molec. Graphics 14:29-32
15. Kyte, J., Doolittle, R.F. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:105-132
16. London, E., Ulbrandt, N.D., Tortorella, D., Jiang, J.X., Abrams, F.S. 1993. Insights into membrane protein folding and translocation from the behavior of bacterial toxins: models for membrane translocation. Society of General Physiologists Series 48:45-61
17. Maduke, M., Roise, D. 1993. Import of a mitochondrial presequcnce into protein-free phospholipid vesicles. Science 260:364-366
18. McClare, C.W. 1971. An accurate and convenient organic phosphorous assay. Analyt. Biochem. 39:527-530
19. Mel, S.F., Stroud, R.M. 1993. Colicin Ia inserts into negatively charged membranes at low pH with tertiary but little secondary structural change. Biochemistry 32:2082-2089
20. Mindell, J.A., Silverman, J.A., Collier, R.J., Finkelstein, A. 1994a. Structure function relationships in diphtheria toxin channels: 11. A residue responsible for the channel's dependence on trans pH. J. Membrane Biol. 137:29-44
21. Mindell, J.A., Silverman, J.A., Collier, R.J., Finkelstein, A. 1994b. Structure-function relationships in diphtheria toxin channels: III. Residues which affect the cis pH dependence of channel conductance. J. Membrane Biol. 137:45-57
22. Mindell, J.A., Zhan, H., Huynh, P.D., Collier, R.J., Finkelstein, A. 1994c. Reaction of diphtheria toxin channels with sulfhydryl-specific reagents: observation of chemical reactions at the single molecule level. Proc. Natl. Acad. Sci. USA 91:5272-5276
23. Oh, K.J., Zhan, H., Cui, C., Hideg, K., Collier, R.J., Hubbell, W.J. 1996. Organization of diphtheria toxin T domain in bilayers: a site-directed spin labeling study. Science 273:810-812
24. O'Keefe, D.O., Cabiaux, V., Choe, S., Eisenberg, D., Collier, R.J. 1992. pH-dependent insertion of proteins into membranes: B-chain mutation of diphtheria toxin that inhibits membrane translocation, Glu-349-Lys. Proc. Natl Acad. Sci. USA 89:6202-6206
25. Sahin-Toth, M., Persson, B., Schweiger, J., Cohan, P., Kaback, H.R. 1994. Cysteine scanning mutagenesis of the N-terminal 32 amino acid residues in the lactose permease of Escherichia coli. Protein Science 3:240-247
26. Schmid, F.X. 1993. Prolyl isomerase: enzymatic catalysis of slow protein-folding reactions. Annu. Rev. Biophys. Biomol. Struct. 22:12.3-142
27. Shen, W.H., Choe, S., Eisenberg, D., Collier, R.J. 1994. Participation of lysine 516 and phenylalanine 530 of diphtheria toxin in receptor recognition. J. Biol. Chem. 269:29077-29084
28. Silverman, J.A., Mindell, J.A., Zhan, H., Finkelstein, A., and Collier, R.J. 1994. Structure-function relationships in diphtheria toxin channels: I. Determining a minimal channel-forming domain. J. Membrane Biol. 137:17-28
29. Steinberg, I.Z., Harrington, W.F., Berger, A., Sela, M., Katchalski, E. 1960. The configurational changes of poly-L-proline in solution. J. Amer. Chem. Soc. 82:5263-5279
30. Studier, F.W., Mofat, B.A. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:113-130
31. Wang, Y., Malenbaum, S.E., Kachel, K., Zhan, H., Collier, R.J., London, E. 1997. Identification of shallow and deep membrane-penetrating forms of diphtheria toxin T domain that are regulated by protein concentration and bilayer width. J. Biol. Chem. 272:25091-25098
32. Yaron, A., Naider, F. 1993. Proline-dependent structural and biological properties of peptides and proteins. Critical Rev. Biochem. Mol. Biol. 28:31-81
33. Zhan, H., Choe, S., Huynh, P.D., Finkelstein, A., Eisenberg, D., Collier, R.J. 1994. Dynamic transitions of the transmembrane domain of diphtheria toxin: disulfide trapping and fluorescence proximity studies. Biochemistry 33:11254-11263
34. Zhan, H., Oh, K.J., Shin, Y.-K., Hubbell, W.L., Collier, R.J. 1995. Interaction of the isolated transmembrane domain of diphtheria toxin with membranes. Biochemistry 34:4856-4863