Generation of epitope-tagged proteins by inverse PCR mutagenesis

BioTechniques

Volumn 26, Issue 5, 1999, Pages 814-816

  Gama, Lucio ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE;

AMINO ACID SEQUENCE; ARTICLE; DELETION MUTANT; DNA SEQUENCE; EPITOPE MAPPING; METHYLATION; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS;

EID: 0032936752     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99265bm03     Document Type: Article

References (8)

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3. Fisher, C.L. and G.K. Pei. 1997. Modification of a PCR-based site-directed mutagenesis . method. BioTechniques 23:570-574.
4. Hcmsley, A., N. Arnheim, M.D. Toney, G. Cortopassi and DJ. Galas. 1989. A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Res. 77:6545-6551.
5. Pont-Kingdon, G. 1994. Construction of chimeric molecules by a two-step recombinant PCR method. BioTechniques 76:1010-1011.
6. Shiio, Y., M. Itoh and J. Inouc. 1995. Epitope tagging. Methods Enzymol. 254:497-502.
7. Stemmer, W.P.C. and S.K. Morris. 1992. Enzymatic inverse PCR: a restriction site independent, single-fragment method for highefficiency, site-directed mutagenesis. BioTechniques 73:214-220.
8. Weiner, M.P., G.L. Costa, W. Schlottin, J. Cline, E. Mathur and J.C. Bauer. 1994. Site-directed mutagenesis of double-stranded DNA by the polymerase chain reaction. Gene 757:119-123.