Isolation of intact protein storage vacuoles from Barley Aleurone: Identification of aspartic and cysteine proteases

Plant Physiology

Volumn 110, Issue 2, 1996, Pages 521-529

  Bethke, Paul C ^a   Hillmer, Stefan ^b   Jones, Russell L ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LEIBNIZ INSTITUTE OF PLANT GENETICS AND CROP PLANT RESEARCH IPK   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

HORDEUM; HORDEUM VULGARE; HORDEUM VULGARE SUBSP. VULGARE;

EID: 0030038126     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.110.2.521     Document Type: Article

References (34)

1. Arnalte M-E, Cornejo M-J, Bush DS, Jones RL (1991) Gibberellic acid stimulates lipid metabolism in barley aleurone protoplasts. Plant Sci 77: 223-232
2. 2+-calmodulin modulates ion channel activity in storage protein vacuoles of barley aleurone cells. Plant Cell 6: 277-285
3. 2+-stimulated secretion of a-amylase during development in barley aleurone protoplasts. Plant Physiol 82: 566-574
4. Bush DS, Jones RL (1987) Measurement of cytoplasmic calcium in aleurone protoplasts using indo-1 and fura-2. Cell Calcium 8: 455-472
5. Chrispeels MJ, Daniels MJ, Maurel C (1995) Aquaporins facilitate water transport through the plasma membrane and the tonoplast. J Cell Biochem 19A: 130
6. Clutterbuck VJ, Briggs DE (1974) Phosphate mobilization in grains of Hordeum disticon. Phytochemistry 13: 45-54
7. Donhowe ET, Peterson DM (1983) Isolation and characterization of oat aleurone and starchy endosperm protein bodies. Plant Physiol 71: 519-523
8. Enari T-M, Sopanen T (1986) Mobilization of endosperm reserves during germination of barley. J Inst Brew 92: 25-31
9. Fernandez DE, Staehelin LA (1985) Structural organization of ultrarapidly frozen barley aleurone cells actively involved in protein secretion. Planta 165: 455-468
10. 18 of barley a-amylase induced by gibberellic acid. Proc Natl Acad Sci USA 58: 1520-1526
11. Fincher GB (1989) Molecular and cellular biology associated with endosperm mobilization in germinating cereal grains. Annu Rev Plant Physiol Plant Mol Biol 40: 305-346
12. Gabard KA, Jones RL (1986) Localization of phytase and acid phosphatase isoenzymes in aleurone layers of barley. Physiol Plant 67: 182-192
13. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151-158
14. Hammerton RW, Ho T-HD (1986) Hormonal regulation of the development of protease and carboxypeptidase activities in barley aleurone layers. Plant Physiol 80: 692-697
15. Holwerda BC, Galvin NJ, Baranski TJ, Rogers JC (1990) In vitro processing of aleurain, a barley vacuolar thiol protease. Plant Cell 2: 1091-1106
16. Holwerda BC, Rogers JC (1992) Purification and characterization of aleurain. Plant Physiol 99: 848-855
17. Jacobsen JV, Knox RB, Pyliotis NA (1971) The structure and composition of aleurone grains in the barley aleurone layer. Planta 101: 189-209
18. Jacobsen, JV, Varner JE (1967) Gibberellic acid induced synthesis of protease by isolated aleurone layers of barley. Plant Physiol 42: 1596-1600
19. Jacobsen JV, Zwar JA, Chandler PM (1985) Gibberellic acid responsive protoplasts from mature aleurone of Himalaya barley. Planta 163: 430-438
20. Johnson KD, Herman EM, Chrispeels MJ (1989) An abundant, highly conserved tonoplast protein in seeds. Plant Physiol 91: 1006-1013
21. Jones RL (1969) The fine structure of barley aleurone cells. Planta 85: 359-374
22. Jones RL, Jacobsen JV (1983) Calcium regulation of the secretion of α-amylase isoenzymes and other proteins from barley aleurone layers. Planta 158: 1-9
23. Koehler S, Ho T-HD (1988) Purification and characterization of gibberellic acid-induced cysteine endoprotease in barley aleurone layers. Plant Physiol 87: 95-103
24. Koehler S, Ho T-HD (1990) A major gibberellic acid-induced barley aleurone cysteine protease which digests hordein. Plant Physiol 94: 251-258
25. Ory RL, Henningsen KW (1969) Enzymes associated with protein bodies isolated from ungerminated barley seeds. I. Purification and some physical properties of a 30 kilodalton cysteine proteinase from green malt. Plant Physiol 44: 1488-1498
26. Poulle M, Jones BL (1988) A proteinase from germinating barley. Plant Physiol 88: 1454-1460
27. Rocher A, Degan FD, Cameron-Mills V, Wettstein DV (1994) Expression and processing of barley serine carboxypeptidases in barley and yeast. J Cell Biochem 19A: 158
28. Runeberg-Roos P, Kervinen J, Kovaleva V, Raikhel NV, Gal S (1994) The aspartic protease of barley is a vacuolar enzyme that processes probarley lectin. Plant Physiol 105: 321-329
29. Sarkkinen P, Kalkkinen P, Tilgmann C, Siuro J, Kervinen J, Mikola L (1992) Aspartic protease from barley grains is related to mammalian lysosomal cathepsin D. Planta 186: 317-323
30. Törmäkangas K, Kervinen J, Östman A, Teeri T (1994) Tissue-specific localization of aspartic proteinase in developing and germinating barley grains. Planta 195: 116-125
31. Villinger W (1991) Lowicryl resins, in MA Hayat, ed, Colloidal Gold, Vol 3. Academic Press, San Diego, CA, pp 59-71
32. Wrobel R, Jones BL (1992) Appearance of endoproteolytic enzymes during the germination of barley. Plant Physiol 100: 1508-1516
33. Wrobel R, Jones BL (1993) Identification and partial characterization of high Mr neutral proteinases from 4-day germinated barley seed. J Cereal Sci 18: 225-237
34. Yupsanis T, Burgess SR, Jackson PJ, Shewry PR (1990) Characterization of the major protein component from aleurone cells of barley (Hordeum vulgare L.). J Exp Bot 41: 385-392