Molecular characterization of eutF mutants of Salmonella typhimurium LT2 identifies eutF lesions as partial-loss-of-function tonB alleles

Journal of Bacteriology

Volumn 181, Issue 2, 1999, Pages 368-374

  Thomas, Michael G ^a   O'Toole, George A ^a,b   Escalante Semerena, Jorge C ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; CARRIER PROTEIN; COBALAMIN; ETHANOLAMINE; HYBRID PROTEIN; HYDROLYASE; IRON; MONOCLONAL ANTIBODY; PROPIONIC ACID; PROPYLENE GLYCOL; SIDEROPHORE; SUCCINIC ACID; TRANSCRIPTION FACTOR;

ALLELE; ARTICLE; BACTERIUM MUTANT; CONTROLLED STUDY; DNA SEQUENCE; ENERGY METABOLISM; ESCHERICHIA COLI; GENE LOCUS; IMMUNOBLOTTING; NONHUMAN; OPERON; PHENOTYPE; PRIORITY JOURNAL; SALMONELLA TYPHIMURIUM;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0032926179     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.2.368-374.1999     Document Type: Article

References (51)

1. Ahmer, B. M., M. G. Thomas, R. A. Larson, and K. Postle. 1995. Characterization of the exbBD operon of Escherichia coli and the role of ExbB and in TonB function and stability. J. Bacteriol. 177:4742-4747.
2. Altman, E., J. R. Roth, A. Hessel, and K. E. Sanderson. 1996. Transposons currently in use in genetic analysis of Salmonella species, p. 2613-2626. In F. C. Neidhardt, R. Curtis III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 2, American Society for Microbiology, Washington, D.C.
3. Anton, M., and K. J. Heller. 1991. Functional analysis of a C-terminally altered TonB protein of Escherichia coli. Gene 105:23-29.
4. 12 uptake in Escherichia coli, J. Bacteriol. 132:796-805.
5. 12-dependent ethanolamine ammonia-lyase and its concerted induction in Escherichia coli. Biochem. J. 176:751-757.
6. Blattner, F. R., G. Plunkett III, C. A. Bloch, N. T. Perna, V. Burland, M. Riley, J. Collado-Vides, J. D. Glasner, C. K. Rode, G. F. Mayhew, J. Gregor, N. W. Davis, H. A. Kirkpatrick, M. A. Goeden, D. J. Rose, B. Mau, and Y. Shao. 1997. The complete genome sequence of Escherichia coli K-12. Science 277:1453-1474.
7. Bradbeer, C. 1965. The clostridial fermentation of choline and ethanolamine. II. Requirement for a cobamide coenzyme by an ethanolamine deaminase. J. Biol. Chem. 240:4675-4581.
8. 12-dependent enzyme ethanolamine deaminase in Salmonella. Nature 254:150-151.
9. Davis, R. W., D. Botstein, and J. R. Roth. 1980. A manual for genetic engineering: advanced bacterial genetics. Cold Spring Harbor Laboratory. Cold Spring Harbor, N.Y.
10. 12 transport in Escherichia coli. J. Bacteriol. 167:920-927.
11. Earhart, C. F. 1996. Uptake and metabolism of iron and molybdenum, p. 1075-1090. In F. C. Neidhardt, R. Curtis III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. I. American Society for Microbiology, Washington, D.C.
12. Elliott, T., and J. R. Roth. 1988. Characterization of Tn10d-Cam: a transposition-defective Tn10 specifying chloramphenicol resistance. Mol. Gen. Genet. 215:332-338.
13. Escalante-Semerena, J. C. Unpublished results.
14. Escalante-Semerena, J. C., and J. R. Roth. 1987. Regulation of cobalamin biosynthetic operons in Salmonella typhimurium. J. Bacteriol. 169:2251-2258.
15. Escalante-Semerena, J. C., S.-J. Suh, and J. R. Roth. 1990. cobA function is required for both de novo cobalamin biosynthesis and assimilation of exogenous corrinoids in Salmonella typhimurium. J. Bacteriol. 172:273-280.
16. Hannavy, K. G., C. Barr, C. J. Dorman, J. Adamson, L. R. Mazengera, M. P. Gallagher, J. S. Evans, B. A. Levine, I. P. Trayer, and C. F. Higgins. 1990. TonB protein of Salmonella typhimurium: a model for signal transduction between membranes. J. Mol. Biol. 216:987-910.
17. Hantke, K. 1981. Regulation of ferric iron transport in Escherichia coli K12: isolation of a constitutive mutant. Mol. Gen. Genet. 182:288-292.
18. 12 receptor protein in the outer membrane of Escherichia coli. J. Bacteriol. 161:904-908.
19. Hiles, I. D., L. M. Powell, and C. F. Higgins. 1987. Peptide transport in Salmonella typhimurium: molecular cloning and characterization of the oligopeptide permease genes. Mol. Gen. Genet. 206:101-109.
20. 12) de novo under anaerobic growth conditions. J. Bacteriol. 159:206-213.
21. Jones, P. W., and J. M. Turner. 1984. A model for the common control of enzymes of ethanolamine catabolism in Escherichia coli. J. Gen. Microbiol. 130:849-860.
22. Laemmli, U. K. 1970. Cleavage and structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
23. Larsen, R. A., P. S. Myers, J. T. Skare, C. L. Seachord, R. P. Darveau, and K. Postle. 1996. Identification of TonB homologs in the family Enterobacteriaceae and evidence for conservation of TonB-dependent energy transduction complexes. J. Bacteriol. 178:1363-1373.
24. 12) biosynthetic genes of Escherichia coli. J. Bacteriol. 177:6371-6380.
25. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
26. Nichols, B. P., and C. Yanofsky. 1979. Nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli: an evolutionary comparison. Proc. Natl. Acad. Sci. USA 76:5244-5248.
27. O'Toole, G. A., and J. C. Escalante-Semerena. 1994. Ph.D. thesis. University of Wisconsin - Madison, Madison, Wis.
28. O'Toole, G. A., and J. C. Escalante-Semerena. 1991. Identification and initial characterization of the eutF locus of Salmonella typhimurium. J. Bacteriol. 173:5168-5172.
29. 12). p. 710-720. In F. C. Neidhardt, R. Curtis III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 1. American Society for Microbiology, Washington, D.C.
30. O'Toole, G. A., M. G. Thomas, and J. C. Escalante-Semerena. 1996. Unpublished results.
31. Postle, K. 1990. Aerobic regulation of the Escherichia coli tonB gene by changes in iron availability and the fur locus. J. Bacteriol. 172:2287-2293.
32. Postle, K. 1993. TonB protein and energy transduction between membranes. J. Bioenerg. Biomembr. 25:591-601.
33. Postle, K., and W. Reznikoff. 1979. Identification of the Escherichia coli tonB gene product in minicells containing tonB hybrid plasmids. J. Mol. Biol. 131:619-636.
34. Rioux, C. R., M. J. Friedrich, and R. J. Kadner. 1990. Genes of the 90-kilobase plasmid of Salmonella typhimurium confer low-affinity cobalamin transport: relationship to fimbria biosynthesis genes. J. Bacteriol. 172:6217-6222.
35. Roof, D. M., and J. R. Roth. 1992. Autogenous regulation of ethanolamine utilization by a transcriptional activator of the eut operon in Salmonella typhimurium. J. Bacteriol. 174:6634-6643.
36. Roof, D. M., and J. R. Roth. 1988. Ethanolamine utilization in Salmonella typhimurium. J. Bacteriol. 170:3855-3863.
37. 12-dependent ethanolamine utilization in Salmonella typhimurium. J. Bacteriol. 171:3316-3323.
38. Roof, S. K., J. D. Allard, K. P. Bertrand, and K. Postle. 1991. Analysis of Escherichia coli TonB membrane topology by use of PhoA fusions. J. Bacteriol. 173:5554-5557.
39. Sanderson, K. E., A. Hessel, S.-H. Liu, and K. E. Rudd. 1996. The genetic map of Salmonella typhimurium, edition VIII, p. 1903-1999. In F. C. Neidhardt, R. Curtis III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 2. American Society for Microbiology, Washington, D.C.
40. Sanderson, K. E., A. Hessel, and K. E. Rudd. 1995. Genetic map of Salmonella typhimurium, edition VIII. Microbiol. Rev. 59:241-303.
41. Sanderson, K. E., and J. R. Roth. 1988. Linkage map of Salmonella typhimurium, edition VII. Microbiol. Rev. 52:485-532.
42. Schmieger, H. 1971. A method for detection of phage mutants with altered transduction ability. Mol. Gen. Genet. 100:378-381.
43. Schmieger, H., and H. Bakhaus. 1973. The origin of DNA in transducing particles of P22 mutants with increased transduction frequencies (HT-mutants). Mol. Gen. Genet. 120:181-190.
44. Schwyn, B., and J. B. Neilands. 1987. Universal assay for the detection and determination of siderophores. Anal. Biochem. 160:47-56.
45. Sheppard, D. E., and J. R. Roth. 1994. A rationale for autoinduction of a transcriptional activator: etnanolamine ammonia-lyase (EutBC) and the operon activator (EutR) compete for adenosyl-cohalamin in Salmonella typhimurium. J. Bacteriol. 176:1287-1296.
46. Skare, J. T., B. M. M. Ahmer, C. L. Seachord, R. P. Darveau, and K. Postle. 1993. Energy transduction between membranes. TonB, a cytoplasmic membrane protein, can be chemically cross-linked in vivo to the outer membrane receptor FepA. J. Biol. Chem. 268:16302-16308.
47. Stocker, B. A. D., M. Nurminen, and P. H. Makela. 1979. Mutants defective in the 33K outer membrane protein of Salmonella typhimurium. J. Bacteriol. 139:376-383.
48. Stojiljkovic, I., A. J. Bäumler, and F. Heffron. 1995. Ethanolamine utilization in Salmonella typhimurium: nucleotide sequence, protein expression, and mutational analysis of the cchA cchB eutE eutj eutH gene cluster. J. Bacteriol. 177:1357-1366.
49. Vogel, H. J., and D. M. Bonner. 1956. Acetylornithase of Escherichia coli: partial purification, and some properties. J. Biol. Chem. 218:97-106.
50. Way, J. C., M. A. Davis, D. Morisato, D. E. Roberts, and N. Kleckner. 1984. New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition. Gene 32:369-379.
51. Young, G. M., and K. Postle. 1994. Repression of tonB transcription during anaerobic growth requires Fur binding at the promoter and a second factor binding upstream. Mol. Microbiol. 11:943-954.