Immunization with an affinity purified protein extract from the adult parasite protects lambs against infection with Haemonchus contortus

Parasite Immunology

Volumn 21, Issue 4, 1999, Pages 201-210

  Knox, D P ^a   Smith, S K ^a   Smith, W D ^a  

^a MOREDUN RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Cysteine proteases; Haemonchus contortus; Vaccination

Indexed keywords

CYSTEINE PROTEINASE; MEMBRANE PROTEIN;

ANIMAL EXPERIMENT; ANTIGEN BINDING; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVITY; GLYCOSYLATION; HAEMONCHUS CONTORTUS; IMMUNIZATION; LAMB; NONHUMAN; PRIORITY JOURNAL; PROVOCATION TEST; WORM INFECTION;

ANIMALS; ANTIBODIES, HELMINTH; CHROMATOGRAPHY, AFFINITY; CROSS REACTIONS; CYSTEINE ENDOPEPTIDASES; FEMALE; GLYCOPROTEINS; HAEMONCHIASIS; HAEMONCHUS; HELMINTH PROTEINS; INTESTINES; LECTINS; MALE; MEMBRANE PROTEINS; PEPTIDES; SHEEP; SHEEP DISEASES; VACCINATION;

ANIMALIA; GRYPUS; HAEMONCHUS CONTORTUS; HALICHOERES; HALICHOERUS GRYPUS; MARTES ZIBELLINA; NEMATODA; OVIS ARIES; PHOCIDAE; PLEURONECTIDAE; PLEURONECTIFORMES;

EID: 0032920180     PISSN: 01419838     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-3024.1999.00220.x     Document Type: Article

References (26)

1. Boisvenue R.J., Stiff M.I., Tonkinson L.V. & Cox G.N. (1992) Fibrinogen-degrading proteins from Haemonchus contortus used to vaccinate sheep. American Journal of Veterinary Research 53, 1263-1265
2. Cox G.N., Milhausen M. & Hageman R. (1991) Anticoagulant and anthemintic proteins and methods for the production of the same. European Patent Application 0 434 909 A2
3. Dalton J.P., McGonigle S., Rolph T.P. & Andrews S.J. (1996) Induction of protective immunity in cattle against infection with Fasciola hepatica by vaccination with cathepsin 1-like proteinases and with haemoglobin. Infection and Immunity 64, 5066-5074
4. Jasmer D.P. & McGuire T.C. (1991) Protective immunity to a blood-feeding nematode (Haemonchus contortus) induced by parasite gut antigens. Infection and Immunity 59, 4412-4417
5. Jasmer D.P., Perryman L.E., Conder G.A., Crow S. & McGuire T. (1993) Protective immunity to Haemonchus contortus induced by immunoaffinity isolated antigens that share a phylogenetically conserved carbohydrate gut surface epitope. Journal of Immunology 151, 5450-5460
6. Karanu F.N., Rurangirwa F.R., McGuire T.C. & Jasmer D.P. (1993) Haemonchus contortus: Identification of proteases with diverse characteristics in adult worm excretory/secretory products. Experimental Parasitology 77, 362-371
7. Knox D.P. & Kennedy M.W. (1988) Proteinases released by the parasitic larval stages of Ascaris suum and their inhibition by antibody. Molecular and Biochemical Parasitology 28, 207-216
8. Knox D.P., Redmond D.L. & Jones D.G. (1993) Characterisation of proteinases in extracts of adult Haemonchus contortus, the ovine abomasal nematode. Parasitology 106, 395-404
9. Laemmli U.K. (1977) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
10. Longbottom D., Redmond D.L., Russell M. et al. (1997) Molecular cloning and characterisation of a putative aspartate proteinase associated with a gut membrane protein complex from adult Haemonchus contortus. Molecular and Biochemical Parasitology 88, 63-72
11. Munn E.A., Greenwood C.A. & Coadwell W.J. (1987) Vaccination of young lambs by means of a protein fraction extracted from adult Haemonchus contortus. Parasitology 94, 385-397
12. Munn E.A. & Smith T.S. (1990) Production and use of anthelmintic agents and protective proteins. Patent Application PCT/GB90/00416
13. Munn E.A., Smith T.S., Smith H. et al. (1997) Vaccination against Haemonchus contortus with denatured forms of the protective antigen HH. Parasite Immunology 19, 243-248
14. Redmond D.L., Knox D.P., Newlands G. & Smith W.D. (1997) Molecular cloning and characterisation of a developmentally regulated putative metallopeptidase present in a host protective extract of Haemonchus contortus. Molecular and Biochemical Parasitology 85, 77-87
15. Rhoads M.L. & Fetterer R.H. (1995) Developmentally regulated secretion of cathepsin L-like cysteine proteases by Haemonchus contortus. Journal for Parasitology 81, 505-512
16. Salvesen G. & Nagase H. (1989) Inhibition of proteolytic enzymes. In Proteolytic Enzymes, eds R.J. Beynon & J.S. Bond, p. 200, IRL Press, Oxford
17. Schallig H.D.F., Van Leeuwen M.A.W. & Cornelissen A.W.C.A. (1997) Protective immunity induced by vaccination with two Haemonchus contortus excretory secretory proteins in sheep. Parasite Immunology 19, 447-453
18. Smith S.K., Pettit D., Newlands G.F.J., Redmond D.L., Skuce P.J., Knox D.P. & Smith W.D. (1999) Further immunization and biochemical studies with a protective antigen complex from the microvillar membrane of the intestine of Haemonchus contortus. Parasite Immunology 21, 187-199
19. Smith T.S., Munn E.A., Graham M., Tavernor A.S. & Greenwood C.A. (1993) Purification and evaluation of the integral membrane protein HH as a protective antigen against Haemonchus contortus. International Journal for Parasitology 23, 271-280
20. Smith T.S., Munn E.A., Newton S.E. et al. (1997) Cloning and characterisation of a microsomal aminopeptidase from the intestine of the nematode Haemonchus contortus. Biochimica et Biophysica Acta 1338, 295-306
21. Smith W.D. (1993) Protection in lambs immunised with Haemonchus contortus gut membrane proteins. Research in Veterinary Science 54, 1-9
22. Smith W.D. & Smith S.K. (1993) Evaluation of aspects of protection afforded to sheep immunised with a gut membrane protein of Haemonchus contortus. Research in Veterinary Science 55, 1-9
23. Smith W.D., Smith S.K., Murray J. & Knox D.P. (1993) Aspartyl proteinases as vaccines. European Patent Application 93916110.5-2116
24. Smith W.D., Smith S.K. & Murray J.M. (1994) Protection studies with integral membrane protein fractions of Haemonchus contortus. Parasite Immunology 16, 231-241
25. Tort J., Brindley P.J., Knox D.P., Wolfe K.H. & Dalton J.P. (1998) Proteinases and associated genes of parasitic helminths. Advances in Parasitology, in press
26. Woodward M.P., Young W.W. & Bloodgood R.E. (1985) Detection of antibodies specific for carbohydrate epitopes using periodate oxidation. Journal of Immunological Methods 78, 143-153