Assembly of the K40 antigen in Escherichia coli: Identification of a novel enzyme responsible for addition of L-serine residues to the glycan backbone and its requirement for K40 polymerization

Journal of Bacteriology

Volumn 181, Issue 3, 1999, Pages 772-780

  Amor, Paul A ^a   Yethon, Jeremy A ^a   Monteiro, Mario A ^b   Whitfield, Chris ^a  

^a UNIVERSITY OF GUELPH   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; ESCHERICHIA COLI LIPOPOLYSACCHARIDE; GLUCURONIC ACID; GLYCAN; LIPID A; N ACETYLGLUCOSAMINE; POLYCLONAL ANTIBODY; SERINE; TRISACCHARIDE;

ARTICLE; ESCHERICHIA COLI; GENE CLUSTER; NONHUMAN; OPEN READING FRAME; POLYMERIZATION; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA;

EID: 0032916450     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.3.772-780.1999     Document Type: Article

References (47)

1. Alexander, D. C., and M. A. Valvano. 1994. Role of the rfe gene in the biosynthesis of the Escherichia coli O7-specific lipopolysaccharide and other O-specific polysaccharides containing N-acetylglucosamine. J. Bacteriol. 176: 7079-7084.
2. Amor, P. A., and C. Whitfield. 1997. Molecular and functional analysis of genes required for expression of group IB K antigens in Escherichia coli: characterization of the his-region containing gene clusters for multiple cell-surface polysaccharides. Mol. Microbiol. 26:145-161.
3. Binotto, J., P. R. MacLachlan, and P. R. Sanderson. 1991. Electrotransformation of Salmonella typhimurium LT2. Can. J. Microbiol. 37:474-477.
4. Branefors-Helander, P. 1981. Structural studies of the capsular polysaccharide elaborated by Haemophilus influenzae type d. Carbohydr. Res. 97:285-291.
5. Cieslewicz, M., and E. Vimr. 1997. Reduced polysialic acid capsule expression in Escherichia coli K1 mutants with chromosomal defects in kpsF. Mol. Microbiol. 26:237-249.
6. Ciucanu, I., and F. Kerek. 1984. A simple and rapid method for the permethylation of carbohydrates. Carhohydr. Res. 131:209-217.
7. Dengler, T., B. Jann, and K. Jann. 1986. Structure of the serine-containing capsular polysaccharide K40 antigen from Escherichia coli O8:K40:H9. Carbohydr. Res. 150:233-240.
8. Dodgson, C., P. A. Amor, and C. Whitfield. 1996. Distribution of the rol gene encoding the regulator of lipopolysaccharide O-chain length in Escherichia coli and its influence on the expression of group I capsular K antigens. J. Bacteriol. 178:1895-1902.
9. Drummelsmith, J., P. A. Amor, and C. Whitfield. 1997. Polymorphism, duplication, and IS1-mediated rearrangement in the chromosomal his-rfb-gnd region of Escherichia coli strains with group IA capsular K antigens. J. Bacteriol. 179:3232-3238.
10. Drummelsmith, J., and C. Whitfield. Gene functions required for the surface expression of the capsular form of the group I K antigen in Echerichia coli (O9a:K30). Submitted for publication.
11. Dutton, G. G. S., and L. A. S. Parolis. 1989. Polysaccharide antigens of Escherichia coli, p. 223-240. In I. C. M. Dea and S. S. Stivola (ed.), Recent developments in industrial polysaccharides: biological and biotechnological advances. Gordon and Breach Science Publishers, New York, N. Y.
12. Flemming, H.-C., and K. Jann. 1978. Biosynthesis of the O8-antigen of Escherichia coli. Glucose at the reducing end of the polysaccharide and growth of the chain. FEMS Microbiol. Lett. 4:203-205.
13. Franco, A. V., D. Liu, and P. R. Reeves. 1996. A Wzz (Cld) protein determines the chain length of K lipopolysaccharide in Escherichia coli O8 and O9 strains. J. Bacteriol. 178:1903-1907.
14. Gromska, W., and H. Mayer. 1976. The linkage of lysine in the O-specific chains of Proteus mirabilis 1959. Eur. J. Biochem. 62:391-399.
15. BAD, promoter. J. Bacteriol. 177:4121-4130.
16. Hamilton, C. A., M. Aldea, B. K. Washburn, P. Babtizke, and S. R. Kushner. 1989. New method of generating deletions and gene replacements in Escherichia coli. J. Bacteriol. 171:4617-4622.
17. Heinrichs, D. E., J. A. Yethon, P. A. Amor, and C. Whitfield. 1998. The assembly system for the outer core portion of R1 and R4-type lipopolysaccharides of Escherichia coli. The R1 core-specific β-glucosyltransferase provides a novel attachment site for O polysaccharides. J. Biol. Chem. 273: 29497-29505.
18. Hitchcock, P. J., and T. M. Brown. 1983. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154:269-277.
19. Jann, B., and K. Jann. 1990. Structure and biosynthesis of the capsular antigens of Escherichia coli. p. 19-42. In K. Jann and B. Jann (ed.), Bacterial capsules, vol. 150. Springer Verlag, Berlin, Germany.
20. Jann, K., T. Dengler, and B. Jann. 1992. Core-lipid A on the K40 polysaccharide of Escherichia coli O8:K40:H9, a representative of group 1 capsular polysaccharides. Zentbl. Bakteriol. 276:196-204.
21. Jann, K., and B. Jann. 1997. Capsules of Escherichia coli, p. 113-143. In M. Sussman (ed.), Escherichia coli: mechanisms of virulence. Cambridge University Press, Cambridge, United Kingdom.
22. Jann, K., and B. Jann. 1983. The K antigens of Escherichia coli. Prog. Allergy 33:53-79.
23. Joiner, K. A. 1988. Complement evasion by bacteria and parasites. Annu. Rev. Microbiol. 42:201-230.
24. Liu, D., R. Cole, and P. R. Reeves. 1996. An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase. J. Bacteriol. 178: 2102-2107.
25. MacLachlan, P. R., W. J. Keenleyside, C. Dodgson, and C. Whitfield. 1993. Formation of the K30 (group I) capsule in Escherichia coli O9:K30 does not require attachment to lipopolysaccharide lipid A-core. J. Bacteriol. 175: 7515-7522.
26. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory. Cold Spring Harbor, N.Y.
27. Mutharia, L. M., B. T. Raymond, T. R. deKievit, and R. M. W. Stevenson. 1993. Antibody specificities of polyclonal rabbit and rainbow trout antisera against Vibrio ordalii and Vibrio anguillarum. Can. J. Microbiol. 39:492-499.
28. Pearce, R., and I. S. Roberts. 1995. Cloning and analysis of gene clusters for production of the Escherichia coli K10 and K54 antigens: identification of a new group of ser.4-linked capsule gene clusters. J. Bacteriol. 177:3992-3997.
29. Radziejewska-Lebrecht, J., A. S. Shashkov, A. Y. Chernyak, and H. Mayer. 1995. Structure and epitope characterization of the O-specific polysaccharides of Proteus mirabilis O28 containing amides of D-galacturonic acid with L-serine and L-lysine. Eur. J. Biochem. 230:705-712.
30. Rick, P. D., G. L. Hubbard, and K. Barr. 1994. Role of the rfe gene in the synthesis of the O8 antigen in Escherichia coli K-12. J. Bacteriol. 176:2877-2884.
31. Rietschel, E. T., L. Brade, B. Lindner, and U. Zähringer. 1992. Molecular biochemistry of lipopolysaccharides, p. 3-42. In D. C. Morrison and J. L. Ryan (ed.), Bacterial endotoxic lipopolysaccharides, vol. I. CRC Press, Boca Raton, Fla.
32. Roberts, I. S. 1996. The biochemistry and genetics of capsular polysaccharide production in bacteria. Annu. Rev. Microbiol. 50:285-315.
33. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
34. Sidorczyk, Z., A. Swierzko, Y. A. Knirel, E. V. Vinogradov, A. Y. Chernyak, L. O. Kononov, M. Cedzynski, A. Rozalski, W. Kaca, A. S. Shashkov, and N. K. Kochetkov. 1995. Structure and epitope specificity of the O-specitic polysaccharide of Proteus penneri strain 12 (ATCC 33519) containing the amide of D-galacturonic acid with L-threonine. Eur. J. Biochem. 230:713-721.
35. Sidorezyk, Z., A. Swierzko, E. V. Vinogradov, Y. A. Knirel, and A. S. Shashkov. 1994. Structural and immunochemical studies on O-specific polysaccharide of Proteus penneri strain 14. Arch. Immunol. Ther. Exp. 42:209-215.
36. Slauch, J. M., M. J. Mahan, P. Michetti, M. R. Neutra, and J. J. Mekalanos. 1995. Acetylation (O-factor 5) affects the structural and immunological properties of Salmonella typhimurium lipopolysaccharide O antigen. Infect. Immun. 63:437-441.
37. Sugiyama, T., N. Kido, Y. Kato, N. Koide, T. Yoshida, and T. Yokochi. 1998. Generation of Escherichia coli O9a serotype, a subtype of E. coli O9, by transfer of the wb* gene cluster of Klebsiella O3 into E. coli via recombination. J. Bacteriol. 180:2775-2778.
38. Towbin, M. T., T. Staehelin, and G. Gordon. 1979. Electrophoretic transfer of proteins from acrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
39. Tsai, G. M., and C. E. Frasch. 1982. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119:115-119.
40. Tsui, F. P., R. Schneerson, R. A. Boykins, A. B. Karpas, and W. Egan. 1981. Structural and immunological studies of the Haemophilus influenzae type d capsular polysaccharide. Carbohydr. Res. 97:293-306.
41. Vinogradov, E. V., W. Kaca, A. S. Shashkov, D. Krajewska-Pietrasik, A. Rozalski, Y. A. Knirel, and N. K. Kochetkov. 1990. The structure of Proteus mirabilis O3 O-specific polysaccharide containing N-(2-hydroxyethyl)-D-alanine. Eur. J. Biochem. 188:645-651.
42. Vinogradov, E. V., D. Krajewska-Pietrasik, W. Kaca, A. S. Shashkov, Y. A. Knirel, and N. K. Kochetkov. 1989. Structure of Proteus mirabilis O27 O-specific polysaccharide containing amino acids and phosphoethanolamine. Eur. J. Biochem. 185:645-650.
43. Westphal, O., and K. Jann. 1965. Bacterial lipopolysaccharide extraction with phenol-water and further applications of the procedure. Methods Carbohydr. Chem. 5:83-91.
44. Whitfield, C. 1995. Biosynthesis of lipopolysaccharide O-antigens. Trends Microbiol. 3:178-185.
45. Whitfield, C., P. A. Amor, and R. Köplin. 1997. Modulation of the surface architecture of Gram-negative bacteria by the action of surface polymer:lipid A-core ligase and by determinants of polymer chain length. Mol. Microbiol. 23:629-638.
46. Whitfield, C., W. J. Keenleyside, and B. R. Clarke. 1994. Structure, function and synthesis of cell surface polysaccharides in Escherichia coli, p. 437-494. In C. L. Gyles (ed.), Escherichia coli in domestic animals and man. CAB International, Tucson, Ariz.
47. Yethon, J. A., D. E. Heinrichs, M. A. Monteiro, M. B. Perry, and C. Whitfield. 1998. Involvement of waaY, waaQ, and waaP in the modification of Escherichia coli lipopolysaccharide, and their role in the formation of a stable outer membrane. J. Biol. Chem. 273:26310-26316.