Comparison of the enzyme-linked immunosorbant assay III, recombinant immunoblot third generation assay, and polymerase chain reaction method in the detection of hepatitis C virus infection in haemodialysis patients

Journal of Clinical Laboratory Analysis

Volumn 13, Issue 3, 1999, Pages 122-125

  Garinis, George ^a,b,f   Spanakis, N ^a,b   Theodorou, V ^a,b   Gorgoulis, V ^e   Manolis, E ^d   Karameris, A ^c   Valis, D ^a,b  

^a MEDICANALYSIS   (Greece)

^b HYGEIA HOSPITAL   (Greece)

^c 401 GENERAL MILITARY HOSPITAL OF ATHENS   (Greece)

^d UNIVERSITY OF ATHENS   (Greece)

^e UNIVERSITY OF ATHENS MEDICAL SCHOOL   (Greece)

^f NONE   (Greece)

Author keywords

Anti HCV; Haemodialysis; HCV; HCV RNA; Serotyping

Indexed keywords

ADULT; ARTICLE; DIAGNOSTIC VALUE; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HEMODIALYSIS; HEPATITIS C VIRUS; HUMAN; IMMUNOBLOTTING; INTERMETHOD COMPARISON; LABORATORY DIAGNOSIS; MAJOR CLINICAL STUDY; MALE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS DIAGNOSIS; VIRUS HEPATITIS; VIRUS INFECTION; VIRUS LOAD;

ADULT; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HEPACIVIRUS; HEPATITIS C; HEPATITIS C ANTIBODIES; HUMANS; IMMUNOBLOTTING; MALE; MIDDLE AGED; RENAL DIALYSIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL;

EID: 0032916310     PISSN: 08878013     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2825(1999)13:3<122::AID-JCLA6>3.0.CO;2-A     Document Type: Article

References (27)

1. Choo QL, Weiner AJ, Overby LR, Kuo G. Houghton M, Bradley DW. Hepatitis C virus: the major causative agent of viral non-a, non-b hepatitis. Br Med Bull 1990;46:423-441.
2. Corcoran GD, Brink NS, Millar CG, et al. Hepatitis C virus infection in haemodialysis patients: a clinical and virological study. J Infect 1994;28:279-285.
3. Mondelli MU, Smedile V, Piazza V, et al. Abnormal alanine aminotransferase activity reflects exposure to hepatitis C virus in haemodialysis patients. Nephrol Dial Transplant 1991;6:480-483.
4. McHutchinson JG, Person JL, Govindarajan S, et al. Improved detection of hepatitis C virus antibodies in high risk populations. Hepatology 1992;15:19-25.
5. Chaudray RK, Andronov A, MacLean C. Detection of hepatitis C virus infection with recombinant immunoblot assay, synthetic immunoblot assay, and polymerase chain reaction. J Clin Lab Anal 1993;7:164-167.
6. Gish RG, Qian S, Xu YL, et al. Concordance between hepatitis C serotyping assays. J Viral Hepat 1997;4:421-2.
7. Abdelnour GE, Matar GM, Sharara HM, Abdelnour AM. Detection of anti-Hepatitis C virus RNA in Lebanese haemodialysis patients. Eur J Epidemiol 1997;13:863-867.
8. Mitsui T, Iwano K, Masuko K, et al. Hepatitis C virus infection in medical personnel after needlestick accident. Hepatology 1992;16:1109-1114.
9. Puoti M, Zonaro A, Ravaggi A, Marin MG, Castelnuovo F, Cariani E. Hepatitis C virus and antibody response in the clinical course of acute hepatitis C virus infection. Hepatology 1992;16:877-881.
10. Young KK, Archer JJ, Omata M, Resnick R. Detection of Hepatitis C virus RNA by a combined reverse transcriptase-polymerase chain reaction assay. J Clin Microbiol 1993;31:882-886.
11. Wolfe L, Tamatsukuri S, Sayada C. Detection of HCV RNA in serum using a single-tube enzyme PCR in combination with a colorimetric microwell assay, Hepatitis C virus. In: GEMHEP. Hepatitis C virus: new diagnostic tools. London: John Libey; 1994. p 83-84.
12. Krajden M, Zhao J, Bourke C, Scalia V. III P, Lau W. Detection of hepatitis C virus by PCR in second-generation enzyme immunoassay-seropositive blood donors by using matched pairs of frozen plasma and pilot tube sera. J Clin Microbiol 1996;34:2191-12195.
13. Dalekos G, Boumba D, Katopodis K, et al. Absence of HCV viraemia in anti-HCV-negative haemodialysis patients. Nephrol Dial Transplant 1998;13:1804-1806.
14. Schroter M, Feucht HH, Schafer P, Zollner B, Laufs R. High percentage of seronegative HCV infections in haemodialysis patients: the need for PCR. Intervirology 1997;40:227-27.
15. Puoti C, Magrini A, Stati T, et al. Clinical, histological, and virological features of hepatitis C virus with persistently normal or abnormal alanine transaminase levels. Hepatology 1997;26:1393-1398.
16. Bukh J, Purcell RH, Miller RH. Importance of primer selection for the detection of hepatitis C virus RNA with the polymerase chain reaction. Proc Natl Acad Sci USA 1992;89:187-191.
17. Longo MC, Beringer MS, Hartkey GL. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene 1990;93:167-177.
18. De Medina M, Hill M, Sullivan HO, et al. Detection of anti-Hepatitis C virus antibodies in patients undergoing dialysis by utilizing a hepatitis C virus 3.0 assay: correlation with hepatitis C virus RNA. J Lab Clin Med 1998;132:73-75.
19. Hodinka RL. The clinical utility of viral quantitation using molecular methods. Clin Diagn Virol 1998;10:25-47.
20. Shindo M, DiBisceglie AM, Cheung L, et al. Decrease in serum Hepatitis C viral RNA during alpha-interferon therapy for chronic hepatitis C. Ann Intern Med 1991;115:700-704.
21. Kessler H, Santner B, Unlauft F. Quantitation and genotyping of hepatitis C virus RNA in sera of haemodialysis and AIDS patients. Clin Diagn Virol 1996;5:73-78.
22. Rossini A, Ravaggi A, Agostinelli E, et al. Virological characterization and liver histology in HCV positive subjects with normal and elevated ALT levels. Liver 1997;17:133-138.
23. Puoti C, Stati T, Magrini A, et al. Liver histology in anti-HCV positive subjects with normal ALT levels. Ital J Gastroenterol Hepatol 1997;29:383-384.
24. Trepo C, Alonso C, Li JS, Qu D, Laurent F, Vitviski L. Limits of immunoserologic and molecular diagnosis of hepatitis C. Nucl Med Biol 1994;21:419-431.
25. Wattre P. Molecular biology at the service of the daily medical virology. 2. Applications to virological diagnosis. Ann Biol Clin 1997;55:81-91.
26. Manzin A, Bagnarelli, Menzo S, et al. Quantitation of Hepatitis C virus genome molecules in plasma samples. J Clin Microbiol 1994;32:1939-1944.
27. Pawlotsky JM, Lonjon I, Hezode C, et al. What strategy should be used for diagnosis of Hepatitis C virus infection in clinical laboratories? Hepatology 1998;27:1700-1702.