Development of a PCR assay for detection of Yersinia ruckeri in tissues of inoculated and naturally infected trout

Applied and Environmental Microbiology

Volumn 65, Issue 1, 1999, Pages 346-350

  Gibello, A ^a   Blanco, M M ^a   Moreno, M A ^a   Cutuli, M T ^a   Domenech, A ^a   Dominguez L ^a   Fernandez Garayzabal J F ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ACCURACY; ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; INOCULATION; NONHUMAN; POLYMERASE CHAIN REACTION; RAINBOW TROUT; YERSINIA; YERSINIA RUCKERI; YERSINIOSIS;

ANIMALIA; BACTERIA (MICROORGANISMS); NEGIBACTERIA; ONCORHYNCHUS MYKISS; SALMONIDAE; YERSINIA RUCKERI;

EID: 0032909175     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.1.346-350.1999     Document Type: Article

References (22)

1. Arias, C. R., E. Garay, and R. Aznar. 1995. Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water. Appl. Environ. Microbiol. 61:3475-3478.
2. Austin, B., and D. A. Austin. 1993. Bacterial fish pathogens: diseases in farmed and wild fish. 2nd ed., p. 208-216. Ellis Horwood Limited, Chichester, United Kingdom.
3. Busch, R. A., and A. J. Lingg. 1975. Establishment of an asymptomatic carrier state infection of enteric redmouth disease in rainbow trout (Salmo gairdneri). J. Fish Res. Board Can. 32:2429-2432.
4. Coleman, S. S., D. M. Melanson, E. G. Biosca, and J. D. Oliver. 1996. Detection of Vibrio vulnificus biotypes 1 and 2 in eels and oysters by PCR amplification Appl. Environ. Microbiol. 62:1378-1382.
5. Fernández-Garayzábal, J. F. 1995. Molecular identification of pathogenic actinomycetes: a new approach. Microbiol. SEM 11:403-404.
6. Kurones, M. D., C. J. Rodgers, and C. B. Munn. 1993. Yersinia ruckeri, the causal agent of enteric redmouth disease (ERM) in fish. Annu. Rev. Fish Dis. 3:105-125.
7. Gendel, S. M. 1996. Computational analysis of the specificity of 16S rRNA-derived signature sequences for identifying food-related microbes. Food Microbiol. 13:1-15.
8. Greisen, K., M. Loeffelholz, A. Purohit, and D. Leong. 1994. PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. J. Gin. Microbiol. 32:335-351.
9. Hunter, V. A. M. D. Knittel, and J. L. Fryer. 1980. Stress-induced transmission of Yersinia ruckeri infection from carriers to recipient steelhead trout Salmo gairdneri Richardson. J. Fish Dis. 3:467-472.
10. Ibrahim, A., B. M. Goebel, W. Liesack, M. Griffiths, and E. Stackebrandt. 1993. The phylogeny of the genus Yersinia based on 16S rDNA sequences. FEMS Microbiol. Lett. 114:173-178.
11. Izumi, S., and H. Wakabayashi. 1997. Use of PCR to detect Cytophaga psychrophila from apparently healthy juvenile ayu and coho salmon eggs. Fish Pathol. 32:169-173.
12. Lawson, P. A., S. E. Gharbia, H. N. Shah, and D. R. Clark. 1989. Recognition of Fusobacterium nucleatum subgroups Fn-1, Fn-2 and Fn-3 ribosomal RNA gene restriction patterns. FEMS Microbiol. Lett. 65:41-46.
13. Lee, C. H., S. F. Pan, and C. H. Chen. 1995. Sequence of a cloned pR72H fragment and its use for detection of Vibrio parahaemolyticus in shellfish with the PCR. Appl. Environ. Microbiol. 61:1311-1317.
14. Maidak, B. L., N. Larsen, M. J. McCaughey, R. Overbeek, G. J. Olsen, K. Fogel, J. Blandy, and C. R. Woese. 1994. The ribosomal database project. Nucleic Acids Res. 22:3485-3487.
15. McIntosh, D., P. G. Meaden, and B. Austin. 1996. A simplified PCR-based method for the detection of Renibacterium salmoninarum utilizing preparations of rainbow trout (Oncorhynchus mykiss, Walbaum) lymphocytes. Appl. Environ. Microbiol. 62:3929-3932.
16. Mooney, J., E. Powell, C. Clabby, and R. Powell. 1995. Detection of Aeromonas salmonicida in wild Atlantic salmon using a specific DNA probe test. Dis. Aquat. Org. 21:131-135.
17. Nübel, U., F. Garcia-Pichel, and G. Muyzer. 1997. PCR primers to amplify 16S rRNA genes from cyanobacteria. Appl. Environ. Microbiol. 63:3327-3332.
18. Sambrook, J. E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
19. Saulnier, D., and P. de Kinkelin, 1997. Polymerase chain reaction primers for investigations on the causative agent of proliferative kidney disease of salmonids. J. Fish Dis. 20:467-170.
20. Urdaci, M. C., C. Chakroun, D. Fauré, and J. F. Bernardet. 1998. Development of a polymerase chain reaction assay for detection and identification of the fish pathogen Flavobacterium psychrophilum. Res. Microbiol. 149:519-530.
21. White, T. J., R. Madej, and D. H. Persing. 1992. The polymerase chain reaction:clinical applications. Adv. Clin. Chem. 29:161-196.
22. Zlotkin, A., A. Eldar, C. Ghittino, and H. Bercovier. 1998. Identification of Lactococcus garviae by PCR. J. Clin. Microbiol. 36:983-985.