Development of a polymerase chain reaction assay for identification and detection of the fish pathogen Flavobacterium psychrophilum

Research in Microbiology

Volumn 149, Issue 7, 1998, Pages 519-530

  Urdaci, M C ^a   Chakroun, C ^b   Faure, D ^a   Bernardet, J F ^b  

^a Lab de Microbiologie et Biochim A   (France)

^b CEMAGREF   (France)

Author keywords

Bacterial diseases; Fish; Flavobacterium psychrophilum; Identification; PCR

Indexed keywords

RNA 16S;

ARTICLE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DIAGNOSTIC VALUE; DNA HYBRIDIZATION; FISH; FLAVOBACTERIUM; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA SEQUENCE; TROUT;

EID: 0032126955     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0923-2508(98)80006-5     Document Type: Article

References (32)

1. Anacker, R.L. & Ordal, E.J. (1955), Study of bacteriophage infecting the myxobacterium Chondrococcus columnaris. J. Bacteriol., 70, 738-741.
2. Anderson, R.L. & Ordal, E.J. (1961), Cytophaga succinicans sp. n., a facultatively anaerobic aquatic myxobacterium. J. Bacteriol., 81, 130-138.
3. Aoki, T. & Hirono, I. (1995), The fish-pathogenic bacterium Pasteurella piscicida detected by the polymerase chain reaction (PCR), in "Proceedings of the Second Symposium on Diseases in Asian Aquaculture", (Shariff, M., Arthur, J.R. & Subasinghe, R.P., eds.), October 1993, Phuket, Thailand.
4. Argenton, F., De Mas, S., Malocco, C., Dalla Valle, L., Giorgetti, G. & Colombo, L. (1996), Use of random DNA amplification to generate specific molecular probes for hybridization tests and PCR-based diagnosis of Yersinia ruckeri. Dis. Aquat. Org., 24, 121-127.
5. Arias, C.R., Garay, E. & Aznar, R. (1995), Nested PCR method for rapid and sensitive detection of Vibrio vulniflcus in fish, sediments, and water. Appl. Environ. Microbiol., 61, 3476-3478.
6. Bernardet, J.-F., Baudin-Laurencin, F. & Tixerant, G. (1988), First identification of Cytophaga psychrophila in France. Bull. Eur. Ass. Fish Pathol., 8, 104-105.
7. Bernardet, J.-F. (1989), Etude phénotypique et génomique des bactéries appartenant aux genres Cytophaga et Flexibacter (ordre des Cytophagales) et comparaison avec le genre Flavobacterium : application à l'identification et à la taxonomie des espèces ichtyopathogènes. Ph. D. Thesis, Université Paris VII.
8. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P. (1996), Cutting a Gordian knot : emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). Int. J. Syst. Bact., 46, 128-148.
9. Brown, L.L., Iwama, G.K., Evelyn, T.P.T., Nelson, W.S. & Levine, R.P. (1994), Use of the polymerase chain reaction (PCR) to detect DNA from Renibacterium salmoninarum within individual salmonid eggs. Dis. Aquat. Org., 18, 165-171.
10. Chakroun, C., Urdaci, M.C., Faure, D., Grimont, F. & Bernardet, J.-F. (1997), Random amplified polymorphic DNA analysis provides rapid differentiation among isolates of the fish pathogen Flavobacterium psychrophilum and between Flavobacterium species. Dis. Aquat. Org., 26, 77-80.
11. Gherna, R. & Woese, C.R. (1992), A partial phylogenetic analysis of the "Flavobacter-Bacteroides" phylum : basis for taxonomic restructuring. System. Appl. Microbiol., 15, 513-521.
12. Gustafson, C.E., Connor, J.T. & Trust, T.J. (1992), Detection of Aeromonas salmonicida from fish by using polymerase chain reaction amplification of the virulence surface array protein gene. Appl. Environ. Microbiol., 58, 3816-3825.
13. Holt, R.A. (1987), Cytophaga psychrophila, the causative agent of bacterial cold-water disease in salmonid fish. Ph.D. thesis, Oregon State University, Corvallis, USA.
14. Higgins, D.G., Bleaby, A.J. & Fuchs, R. (1992), CLUSTAL V, improved software for multiple sequence alignment. Comput. Appl. Biosc., 8, 189-191.
15. Lehmann, J., Mock, D., Stürenberg, F.-J. & Bernardet, J.-F. (1991), First isolation of Cytophaga psychrophila from a systemic disease in eel and cyprinids. Dis. Aquat. Org., 10, 217-220.
16. Iida, Y. & Mizokami, A. (1996), Outbreaks of coldwater disease in wild ayu and pale chub. Fish Pathol., 31, 157-164.
17. Lorenzen, E. & Karas, N. (1992), Detection of Flexibacter psychrophilus by immunofluorescence in fish suffering from fry mortality syndrome : a rapid diagnostic method. Dis. Aquat. Org., 13, 231-234.
18. Magnússon, H.B., Frithjónsson, Ó.H., Andrésson, Ó.S., Benediktsdóttir, E., Guthmunsdóttir, S. & Andrésdóttir, V. (1994), Renibacterium salmoninarum, the causative agent of bacterial kidney disease in salmonid fish, detected by nested reverse transcription-PCR of 16S rRNA sequences. Appl. Environ. Microbiol., 60, 4580-4583.
19. Manz, W., Amann, R., Ludwig, W., Vancanneyt, M. & Schleifer, K.-H. (1996), Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment. Microbiology, 142, 1097-1106.
20. Mauel, M.J., Giovannoni, S.J. & Fryer, J.L. (1996), Development of polymerase chain reaction assays for detection, identification and differentiation of Piscirickettsia salmonis. Dis. Aquat. Org., 21, 131-135.
21. McIntosh, D., Meaden, P.G. & Austin, B. (1996), A simplified PCR-based method for the detection of Renibacterium salmoninarum utilizing preparations of rainbow trout (Oncorhynchus mykiss, Walbaum) lymphocytes. Appl. Environ. Microbiol., 62, 3929-3932.
22. Mooney, J., Powell, E., Clabby, C. & Powell, R. (1995), Detection of Aeromonas salmonicida in wild Atlantic salmon using a specific DNA probe test. Dis. Aquat. Org., 21, 131-135.
23. Nakagawa, Y. & Yamasato, K. (1993), Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J. Gen. Microbiol., 139, 1155-1161.
24. Sammbrook, J., Fritsch, E. F. & Maniatis, T. (1989), Molecular cloning : A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, New York.
25. Segers, P., Mannheim, W., Vancanneyt, M., De Brandt, K., Hinz, K.-H., Kersters, K. & Vandamme P. (1993), Riemerella anatipestifer gen. nov., comb. nov., the causative agent of septicemia anserum exsudativa, and its phylogenetic affiliation within the Flavobacterium-Cytophaga rRNA homology group. Int. J. Syst. Bacteriol., 43, 768-776.
26. Strohl, W.R. & Tait, L.R. (1978), Cytophaga aquatilis sp. nov., a facultative anaerobe isolated from the gills of freshwater fish. Int. J. Syst. Bacteriol., 28, 293-303.
27. Southern, E.M. (1975), Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol., 98, 503-517.
28. Toyama, T., Kita-Tsukamoto, K. & Wakabayashi, H. (1994), Identification of Cytophaga psychrophila by PCR targeted 16S ribosomal DNA. Fish Pathol. 29, 271-275.
29. Toyama, T., Kita-Tsukamoto, K. & Wakabayashi, H. (1996), Identification of Flexibacter maritimus, Flavobacterium branchiophilum and Cytophaga columnaris by PCR targeted 16S ribosomal DNA. Fish Pathol., 31, 25-31.
30. Vandamme, P., Bernardet, J.-F., Segers, P., Kersters, K. & Holmes, B. (1994a), New perspectives in the classification of the Flavobacteria : description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int. J. Syst. Bacteriol., 44, 827-831.
31. Vandamme, P., Segers, P., Vancanneyt, M., Van Hove, K., Mutters, R., Hommez, J., Dewhirst, F., Paster, B., Kersters, K., Falsen, E., Devriese, L.A., Bisgaard, M., Hinz, K.-H. & Mannheim, W. (1994b), Ornithobacterium rhinotracheale gen. nov., sp. nov., isolated from the avian respiratory tract. Int. J. Syst. Bacteriol., 44, 24-37.
32. Weisburg, W.G., Barns, S.M., Pelletier, D.A. & Lane, D.J. (1991), 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol., 173, 697-703.