Green fluorescent proteins with short half-lives as reporters in Dictyostelium discoideum

Development Genes and Evolution

Volumn 209, Issue 1, 1999, Pages 63-68

  Deichsel, Heike ^a   Friedel, Shirin ^a   Detterbeck, Anne ^a   Coyne, Catharine ^a   Hamker, Ursula ^a   MacWilliams, Harry K ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; RIBOSOME PROTEIN; UBIQUITIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL NUCLEUS; DICTYOSTELIUM DISCOIDEUM; HALF LIFE TIME; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN TRANSPORT;

ANIMALS; BETA-GALACTOSIDASE; DICTYOSTELIUM; GENE EXPRESSION; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; PROMOTER REGIONS (GENETICS); RIBOSOMAL PROTEINS; UBIQUITINS;

DICTYOSTELIUM; DICTYOSTELIUM DISCOIDEUM;

EID: 0032900636     PISSN: 0949944X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004270050228     Document Type: Article

References (19)

1. Bachmair A, Varshavsky A (1989) The degradation signal in a short-lived protein. Cell 56: 1019-1032
2. Bachmair A, Finley D, Varshavsky A (1986) In vivo half-life of a protein is a function of its amino-terminal residue. Science 234: 179-186
3. Bottini F, Roos U-P (1992) Nucleic acid cytochemistry of the nucleus and microtubule-organizing centers in Dictyostelium discoideum. Eur J Protistol 28: 184-197
4. Cohen B, Simcox AA, Cohen SM (1993) Allocation of the thoracic imaginal primordia in the Drosphila embryo. Development 117: 597-608
5. Detterbeck A (1998) Untersuchungen der Musterbildung in Dictyostelium discoideum mit Reportern verschiedener Halbwertzeit. Doctoral thesis, Ludwig-Maximilians Universität, München
6. Detterbeck S, Morandini P, Wetterauer B, Bachmair A, Fischer K, MacWilliams HK (1994) The 'prespore-like-cells' of Dictyostelium have ceased to express a prespore gene: analysis using short-lived β-galactosidases as reporters. Development 120: 2847-2855
7. Early A, Williams J (1989) Identification of sequences regulating the transcription of a Dictyostelium gene selectively expressed in prespore cells. Nucleic Acids Res 16: 6473-6484
8. Esch RK, Firtel RA (1991) cAMP and cell sorting control the spatial expression of a developmentally essential cell-type-specific ras gene in Dictyostelium. Genes Dev 5: 9-21
9. Gerisch G, Albrecht R, Heizer C, Hodgkinson S, Maniak M (1995) Chemoattractant-controlled accumulation of coronin at the leading edge of Dictyostelium cells monitored using a green fluorescent protein-coronin fusion protein. Curr Biol 5: 1280-1285
10. Harwood A, Drury L (1990) New vectors for the expression of the E. coli lacZ gene in Dictyostelium. Nucleic Acids Res 18: 4292
11. Heim R, Cubitt AB, Tsien RY (1995) Improved green fluorescence. Nature 373: 663-664
12. Hribar I (1994) Transformation nichtaxenischer Stämme von Dictyostelium discoideum. Doctoral thesis, Ludwig-Maximilians-Universität, München
13. Jermyn K, Williams J (1995) Comparison of the Dictyostelium rasD and ecmA genes reveals two distinct mechanisms whereby an RNA may become enriched in prestalk cells. Differentiation 58: 261-267
14. Johnson ES, Ma PC, Ota IM, Varshavsky A (1995) A proteolytic pathway that recognizes ubiquitin as a degradation signal. J Biol Chem 270: 17442-17456
15. McKeown M, Hirth KP, Edwards C, Firtel RA (1982) Examination of the regulation of the actin multigene family in Dictyostelium discoideum. Prog Clin Biol Res 85A: 51-78
16. Roos U-P, Bottini F, Jenni V (1992) Morphology of the nucleolus in undifferentiated amoebae of Dictyostelium discoideum. Eur J Protistol 28: 94-101
17. Singleton CK, Manning SS, Ken R (1989) Primary structure and regulation of vegetative specific genes of Dictyostelium discoideum. Nucleic Acids Res 17: 9679-9692
18. Wetterauer B, Morandini P, Hribar I, Murgia-Morandini I, Hamker U, Singelton C, MacWilliams HK (1996) Wild-type strains of Dictyostelium discoideum can be transformed using a novel selection cassette driven by the promoter of the ribosomal V18 gene. Plasmid 36: 169-181
19. Zaccaria R, Greco D, MacWilliams H, Bozzaro S, Ceccarelli A (1998) UGUS, a reporter for use with destabilizing N-termini. Nucleic Acid Res 26: 1128-1129