Wild-type strains of Dictyostelium discoideum can be transformed using a novel selection cassette driven by the promoter of the ribosomal V18 gene

Plasmid

Volumn 36, Issue 3, 1996, Pages 169-181

  Wetterauer, Birgit ^a   Morandini, Piero ^b   Hribar, Irene ^a   Murgia Morandini, Irene ^b   Hamker, Ursula ^a   Singleton, Charles ^c   Macwilliams, Harry K ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b Dipartimento di Biologia L Gorini   (Italy)

^c VANDERBILT UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM TRANSFORMATION; DICTYOSTELIUM DISCOIDEUM; GENE CASSETTE; MICROBIAL GROWTH; NONHUMAN; PLASMID; PROMOTER REGION; RIBOSOME; STRAIN DIFFERENCE;

BACTERIA (MICROORGANISMS); DICTYOSTELIUM DISCOIDEUM;

EID: 0030295030     PISSN: 0147619X     EISSN: None     Source Type: Journal    
DOI: 10.1006/plas.1996.0044     Document Type: Article

References (41)

1. ADACHI, H., HASEBE, T., YOSHINAGA, K., OHTA, T., AND SUTOH, K. (1994). Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme-medialed integration of the blasticidin S resistance marker. Biochem. Biophys. Res. Commun. 205, 1808-1877.
2. ALEXANDER, S., CIBUSKY, A.-M., AND CUNEO, S. D. (1986). Multiple regulatory genes control expression of a gene family during development of Dictyostelium discoideum. Mol Cell Biol 6, 4353-4361.
3. ASHWORTH, J. M., AND WATTS, D. J. (1970). Metabolism of the cellular slime mold Dictyostelium discoideum grown in axenic culture. Biochem. J. 119, 175-182.
4. BACHMAIR, A., AND VARSHAVSKY, A. (1989). The degradation signal in a short-lived protein. Cell 56, 1019-1032.
5. BECK, E., LUDWIG, G., AUERSWALD, E. A., REISS, B., AND SCHALLER, H. (1982). Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene 19, 327-336.
6. BLUSCH, J., MORANDINI, P., AND NELLEN, W. (1992). Transcriptional regulation by folate: Inducible gene expression in Dictyostelium transformants during growth and early development. Nucleic Acids Res. 20, 6235-6238.
7. CLARKE, M., AND KAYMAN, S. C. (1987). The axenic mutations and endocytosis in Dictyostelium. Methods Cell Biol. 28, 157-176.
8. COHEN, S. M., KNECHT, D., LODISH, H. F., AND LOOMIS, W. F. (1986). DNA sequences required for expression of a Dictyostelium actin gene. EMBO. J. 5, 3361 -3366.
9. COUKELL, M. B., AND ROXBY, N.M. (1977). Linkage analysis of developmental mutations in aggregation deficient mutants of Dictyostelium discoideum. Mol. Gen. Genet. 151, 275-288.
10. DETTERBECK, S., MORANDINI, P., WETTERAUER, B., BACHMAIR, A., AND MACWILLIAMS, H. K. (1994). The 'prespore-like cells' of Dictyostelium have ceased to express a prespore gene: Analysis using short-lived beta-galactosidases as reporters. Development 120, 2847-2855.
11. DINGERMANN, T., REINDL, N., WERNER, H., HILDE-BRANDT, M., NELLEN, W., HARWOOD, A., WILLIAMS, J., AND NERKE, K. (1989). Optimization and in situ detection of Escherichia coli β-galactosidase gene expression in Dictyostelium discoideum. Gene 85, 353-362.
12. DYNES, J. L., AND FIRTEL, R. A. (1989). Molecular complementation of a genetic marker in Dictyostelium using a genomic DNA library. Proc. Natl. Acad. Sci. USA 86, 7966-7970.
13. EGELHOFF, T. T., BROWN, S. S., MANSTEIN, D. J., AND SPUDICH, J. A. (1989). Hygromycin resistance as a selectable marker in Dictyostelium discoideum. Mol. Cell. Biol. 9, 1965-1968.
14. HARWOOD, A. J., AND DRURY, L. (1990). New vectors for expression of the E. coli lacZ gene in Dictyostelium. Nucleic Acids Res. 18, 4392.
15. HOWARD, P. K., AHERN, K. G., AND FIRTEL, R. A. (1988). Establishment of a transient expression system for Dictyostelium discoideum. Nucleic Acids Res. 16, 2613-2623.
16. HRIBAR, I. (1994). "Transformation nichtaxenischer Stämme von Dictyostelium discoideum." Thesis, Fakultät für Biologie, Ludwig-Maximilians-Universität, Munich.
17. HUGHES, J. E., PODGORSKI, G. J., AND WELKER, D. L. (1992). Selection of Dictyostelium discoideum transformants and analysis of vector maintenance using live bacteria resistant to G418. Plasmid 28, 46-60.
18. JERMYN, K. A., AND WILLIAMS, J. G. (1989). An analysis of culmination in Dictyostelium using prestalk-and stalk-specific markers. Development 111, 779-787.
19. KALPAXIS, D., WERNER, H., MARCOTTE, E. B., JACQUET, M., AND DINGERMANN, T. (1990). Positive selection for Dictyostelium mutants lacking uridine monophosphate synthase activity based on resistance to 5-fluoroorotic acid. Dev. Genet. 11, 396-402.
20. KEN, R., AND SINGLETON, C. K. (1994). Redundant regulatory elements account for the developmental control of a ribosome protein gene from Dictyostelium discoideum. Differentiation 55, 97-103.
21. KNECHT, D. A., COHEN, S. M., LOOMIS, W. F., AND LODISH, H. F. (1986). Developmental regulation of Dictyostelium discoideum actin gene fusions carried on low-copy and high-copy transformation vectors. Mol. Cell. Biol. 6, 3073-3083.
22. LLOYD, M. M., CECCARELLI, A., AND WILLIAMS, J. G. (1990). Establishment of conditions for the transformation of nonaxenic Dictyostelium strains. Dev. Genet. 11, 391-395.
23. MACWILLIAMS, H. K. (1985). Two feedback loops may regulate cell-type proportions in Dictyostelium. Symp. Soc. Quant. Biol. 50, 779-785.
24. MAEDA, Y. (1983). Axenic growth of Dictyostelium discoideum wild-type NC4 cells and its relation to endocytotic ability. J. Gen. Microbiol. 129, 2467-2473.
25. MANIAK, M., AND NELLEN, W. (1990). Two separable promoters control different aspects of expression of a Dictyostelium gene. Nucleic Acids Res. 18, 3211-3217.
26. MANIAK, M., SAM, U., AND NELLEN, W. (1989). A colony-blot technique for the detection of specific transcripts in eukaryotes. Anal. Biochem. 176, 78-81.
27. MARSCHALEK, R., HOFMANN, J., SCHUMANN, G., GOSSERINGER, R., AND DINGERMANN, T. (1992). Structure of DRE, a retrotransposable element which integrates with position specificity upstream of Dictyostelium discoideum tRNA genes. Mol. Cell. Biol. 12, 229-239.
28. MORRISON, A., AND HARWOOD, A. (1992). A simple method of generating axenic derivatives of Dictyostelium strains. Exp. Cell Res. 199, 383-386.
29. NELLEN, W., SILAN, C., AND FIRTEL, R. A. (1984). DNA-mediated transformation in Dictyostelium discoideum: Regulated expression of an actin gene fusion. Mol. Cell. Biol. 4, 2890-2898.
30. NELLEN, W., SILAN, C., SAUR, U., AND FIRTEL, R. A. (1986). Regulatory sequences in the promoter of the Dictyostelium actin 6 gene. EMBO. J. 6, 3367-3372.
31. NEWELL, P. C., AND ROSS, F. M. (1982). Genetic analysis of the slug stage of Dictyostelium discoideum. J. Gen. Microbiol. 128, 1639-1652.
32. ROSS, F. M., AND NEWELL, P. C. (1981). Streamers: Chemotactic mutants of Dictyostelium discoideum with altered cyclic GMP metabolism. J. Gen. Microbiol. 127, 339-350.
33. SAMBROOK, J., FRITSCH, E. F., AND MANIATIS, T. (1989). "Molecular Cloning: A Laboratory Handbook." Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
34. R selection marker induces germination-specific adenylyl cyclase activity in Dictyostelium cells. Exp. Cell Res. 220, 505-508.
35. SINGLETON, C. K., MANNING, S. S., AND KEN, R. (1989). Primary structure and regulation of vegetative specific genes of Dictyostelium discoideum. Nucleic Acids Res. 17, 9679-9692.
36. SUSSMAN, M. (1966). Biochemical and genetic methods in the study of cellular slime mold development. Methods Cell Physiol. 2, 397-410.
37. SUTOH, K. (1994). A transformation vector for Dictyostelium discoideum with anew selectable marker Bsr. Plasmid 30, 150-154.
38. VOCKE, C. D., AND COX, E. C. (1992). Establishment and maintenance of stable spatial patterns in lacZ fusion transformants of Polysphondylium pallidum. Development 115, 59-65.
39. WILCZYNSKA, Z., AND FISHER, P. R. (1994). Analysis of a complex plasmid insertion in a phototaxis-deficient transformant of Dictyostelium discoideum selected on a Micrococcus luteus lawn. Plasmid 32, 182-194.
40. WILLIAMS, K. L., KESSIN, R. H., AND NEWELL, P. C. (1974). Genetics of growth in axenic medium of the cellular slime mould Dictyostelium discoideum. Nature 247, 142-143.
41. WILLIAMS, K. L., AND NEWELL, P. (1976). A genetic study of aggregation in the cellular slime mould Dictyostelium discoideum using complementation analysis. Genetics 82, 287-307.