High expression and steady-state kinetic characterization of methionine site-directed mutants of Escherichia coli methionyl- and selenomethionyl-dihydrofolate reductase

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1429, Issue 2, 1999, Pages 401-410

  Shaw, David ^a   Odom, Jerome D ^a   Dunlap, R Bruce ^a  

^a UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

Dihydrofolate reductase; Overexpression; Purification; Selenomethionine; Steady state kinetics

Indexed keywords

DIHYDROFOLATE REDUCTASE; MUTANT PROTEIN; SELENOMETHIONINE;

ARTICLE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STABILITY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS; STEADY STATE;

ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; KINETICS; METHIONINE; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; SELENOMETHIONINE; TETRAHYDROFOLATE DEHYDROGENASE;

EID: 0032898165     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(98)00250-7     Document Type: Article

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