Engineering specificity for folate into dihydrofolate reductase from Escherichia coli

Biochemistry

Volumn 35, Issue 5, 1996, Pages 1653-1663

  Posner, Bruce A ^a   Li, Luyuan ^b   Bethell, Richard ^c   Tsuji, Tomoko ^d   Benkovic, Stephen J ^e  

^a UNIVERSITY OF TEXAS AT DALLAS   (United States)

^b PFIZER INC   (United States)

^c GLAXOSMITHKLINE   (United Kingdom)

^d SAGAMI CHEMICAL RESEARCH CENTER   (Japan)

^e PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIHYDROFOLATE REDUCTASE; FOLIC ACID; MUTANT PROTEIN;

AMINO ACID COMPOSITION; ARTICLE; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE;

AMINO ACID SEQUENCE; ESCHERICHIA COLI; FOLIC ACID; HYDROGEN-ION CONCENTRATION; KINETICS; LIGANDS; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NADP; PROTEIN ENGINEERING; PROTEIN STRUCTURE, SECONDARY; SPECIES SPECIFICITY; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; TETRAHYDROFOLATE DEHYDROGENASE;

AVES; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FELIS CATUS; VERTEBRATA;

EID: 0030043244     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9518095     Document Type: Article

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