Identification of Tyr438 as the major in vitro c-Src phosphorylation site in human gelsolin: A mass spectrometric approach

Protein Science

Volumn 8, Issue 1, 1999, Pages 234-241

  De Corte, Veerle ^a   Demol, Hans ^a   Goethals, Mark ^a   Van Damme, Jozef ^a   Gettemans, Jan ^a   Vandekerckhove, Joël ^a  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

Author keywords

Electrospray ionization mass spectrometry; Gelsolin; Matrix assisted laser desorption ionization time of flight mass spectrometry; Post source decay; Thin layer chromatography; Tyrosine phosphorylation

Indexed keywords

ACTIN; ACTIN BINDING PROTEIN; GELSOLIN; LYSOPHOSPHATIDIC ACID; PHOSPHOLIPID; PHOSPHOTYROSINE; PROTEIN TYROSINE KINASE; TYROSINE;

ARTICLE; CONTROLLED STUDY; HUMAN; IMMUNOPRECIPITATION; IN VITRO STUDY; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; THIN LAYER CHROMATOGRAPHY;

EID: 0032898117     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.1.234     Document Type: Article

References (45)

1. Baldassare JJ, Henderson PA, Tarver A, Fisher GJ. 1997. Thrombin activation of human platelets dissociates a complex containing gelsolin and actin from phosphatidylinositide-specific phospholipase Cgammal. Biochem J 324:283-287.
2. Banno Y, Nakashima T, Kumada T, Ebisawa K, Nonomura Y, Nozawa Y. 1992. Effects of gelsolin on human platelet cytosolic phosphoinositide-phospholipase C isozymes. J Biol Chem 267:6488-6494.
3. c-src or stimulation of cells with epidermal growth factor induces the stable association of tyrosine-phosphorylated cellular proteins with GTPase-activating protein. Mol Cell Biol 11:945-953.
4. Bradford MM. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.
5. Bretscher A, Weber K. 1979. Villin: The major microfilament associated protein of the intestinal microvillus. Proc Natl Acad Sci USA 76:2321-2325.
6. Burtnick LD, Koepf EK, Grimes J, Jones EY, Stuart DI, McLaughlin PJ, Robinson RC. 1997. The crystal structure of plasma gelsolin: Implications for actin severing, capping, and nucleation. Cell 90:661-670.
7. Chellaiah M, Fitzgerald C, Alvarez U, Hruska K. 1998. c-Src is required for stimulation of gelsolin associated phosphatidylinositol 3-kinase. J Biol Chem 273:11908-11916.
8. Colby S, King T, Reilly J. 1994. Improving the resolution of matrix assisted laser desorption/ionization time-of-flight mass spectrometry by exploiting the correlation between ion position and velocity. Rapid Commun Mass Spectrom 8:865-873.
9. Cooper JA, Bryan J, Schwab B III, Frieden C, Loftus DJ, Elson EL. 1987. Microinjection of gelsolin into living cells. J Cell Biol 104:491-501.
10. Davis S, Lu ML, Lo SH, Lin S, Butler JA, Druker BJ, Roberts TM, An Q, Chen LB. 1991. Presence of an SH2 domain in the actin-binding protein tensin. Science 252:712-715.
11. DeClue JE, Martin GS. 1987. Phosphorylation of talin at tyrosine in Rous sarcoma virus transformed cells. Mol Cell Biol 7:371-378.
12. De Corte V, Gettemans J, De Ville Y, Waelkens E, Vandekerckhove J. 1996. Fragmin, a microfilament regulatory protein from Physarum polycephalum, is phosphorylated by casein kinase II type enzymes. Biochemistry 35:5472-5480.
13. c-src catalyzed phosphorylation of gelsolin and related actin-binding proteins. FEBS Lett 401:191-196.
14. Ellis C, Moran M, McCormick F, Pawson T. 1990. Phosphorylation of GAP and GAP associated proteins by transforming and mitogenic tyrosine kinases. Nature 343:377-381.
15. Fenn J, Mann M, Meng C, Wong S. 1989. Electrospray ionization for mass spectrometry of large biomolecules. Science 246:64-71.
16. Gettemans J, De Ville Y, Waelkens E, Vandekerckhove J. 1995. The actin-binding properties of the Physarum actin-fragmin complex. J Biol Chem 270:2644-2651.
17. Gevaert K, De Mol H, Verschelde JL, Van Damme J, De Boeck S, Vandekerckhove J. 1997. Novel techniques for identification and characterization of proteins loaded on gels in femtomole amounts. J Protein Chem 16:335-342.
18. Haefner B, Baxter R, Fincham VJ, Downs CP, Frame MC. 1995. Cooperation of Src homology domains in the regulated binding of phosphatidylinositol 3-kinase. A role for the Src homology 2 domain. J Biol Chem 270:7937-7943.
19. Hartwig JH, Bokoch GM, Carpenter CL, Janmey PA, Taylor LA, Toker A, Stossel TP. 1995. Thrombin receptor ligation and activated rac uncap actin filament barbed ends through phosphoinositide synthesis in permeabilized human platelets. Cell 82:643-653.
20. Huang C, Ni Y, Wang T, Gao Y, Haudenschild CC, Zhan X. 1997. Down regulation of the filamentous actin crosslinking activity of cortactin by src mediated tyrosine phosphorylation. J Biol Chem 272:13911-13915.
21. Janmey PA, Chaponnier C, Lind SE, Zaner KS, Stossel TP, Yin HL. 1985. Interactions of gelsolin and gelsolin-actin complexes with actin. Effects of calcium on actin nucleation, filament severing, and end blocking. Biochemistry 24:3714-3723.
22. Janmey PA, Stossel TP. 1987. Modulation of gelsolin function by phosphatidyl 4,5-bisphosphate. Nature 325:362-364.
23. Karas M, Hillenkamp F. 1988. Laser desorption ionization of proteins with molecular masses exceeding 10,000 dallons. Anal Chem 60:2299-2301.
24. Krieg J, Hunter T. 1992. Identification of the two major epidermal growth-factor induced tyrosine phosphorylation sites in the microvillar core protein ezrin. J Biol Chem 267:19258-19265.
25. Liscovitch M, Chalifa V, Pertile P, Chen CS, Cantley LC. 1994. Novel function of phosphatidylinositol 4,5-bisphosphate as a cofactor for brain membrane phospholipase D. J Biol Chem 269:21403-21406.
26. 2+ dependent actin filament severing protein from bovine adrenal medulla. J Biol Chem 264:7458-7465.
27. Malsudaira PT, Burgess DR. 1978. SDS microslab linear gradient polyacrylamide gel electrophoresis. Anal Chem 87:386-396.
28. Meerschaert K, De Corte V, De Ville Y, Vandekerckhove J, Gettemans J. 1998. Gelsolin and functionally similar actin-binding proteins are regulated by lysophosphatidic acid. EMBO J 17:5923-5932.
29. Mertens N, Remaut E, Fiers W. 1995. Tight transcriptional control mechanism ensures stable high level expression from T7 promotor based expression plasmids. Bio Technol 13:175-179.
30. Moolenaar WH, Kranenburg O, Postma FR, Zondag GCM. 1997. Lysophosphatidic acid: G-protein signaling and cellular responses. Curr Opin Cell Biol 9:168-173.
31. Mumby SM, Gilman AG. 1991. Synthetic peptide antisera with determined specificity for G protein alpha or beta subunits. Methods Enzymol 195:215-233.
32. Pasquale EB, Maher PA, Singer SJ. 1986. Talin is phosphorylated on tyrosine in chicken embryo fibroblasts transformed by Rous sarcoma virus. Proc Natl Acad Sci USA 83:5507-5511.
33. Petch LA, Bockholt SM, Bouton A, Parsons JT, Burridge K. 1995. Adhesion induced tyrosine phosphorylation of the p130 src substrate. J Cell Sci 108:1371-1379.
34. 2+ sensitive actin monomer binding sites in gelsolin to segment-4 and identification of calcium binding sites. Biochemistry 34:1583-1588.
35. Richardson A, Malik RK, Hildebrand JD, Parsons JT. 1997. Inhibition of cell spreading by expression of the C-terminal domain of focal adhesion kinase (FAK) is rescued by coexpression of Scr or catalytically inactive FAK: A role for paxillin tyrosine phosphorylation. Mol Cell Biol 17:6906-6914.
36. Schlaepfer DD, Hunter T. 1996. Evidence for in vivo phosphorylation of the Grb2 SH2-domain binding site on focal adhesion kinase by Src family protein-tyrosine kinases. Mol Cell Biol 16:5623-5633.
37. Sefton BM, Hunter T, Bal EH, Singer SJ. 1981. Vinculin: A cytoskeletal target of the transforming protein Rous sarcoma virus. Cell 24:165-174.
38. Spengler B, Kirsch D, Kaufmann R, Jaeger E. 1992. Peptide sequencing by matrix assisted laser desorption mass spectrometry. Rapid Commun Mass Spectrom 6:105-108.
39. Spudich JA, Watt S. 1971. The regulation of rabbit skeletal muscle contraction. J Biol Chem 246:4866-4871.
40. Steed PM, Nagar S, Wennogle LP. 1996. Phospholipase D regulation by a physical interaction with the actin-binding protein gelsolin. Biochemistry 35:5229-5237.
41. Sun HQ, Lin KM, Yin HL. 1997. Gelsolin modulates phospholipase C activity in vivo through phospholipid binding. J Cell Biol 138:811-820.
42. Takao T, Gonzalez J, Yoshidome K, Sato K, Asada T, Kammei Y, Shimonishi Y. 1993. Automatic precursor-ion switching in a four-sector tandem mass spectrometer and its application to acquisition of the MS/MS product ions derived from a partially (18)O-labeled peptide for their facile assignments. Anal Chem 65:2394-2399.
43. Towbin H, Staehelin T, Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76:4350-4354.
44. Vandekerckhove J. 1990. Actin-binding proteins. Curr Opin Cell Biol 2:41-50.
45. Zhao Y, Chait BT. 1994. Protein epitope mapping by mass spectrometry. Anal Chem 66:3723-3726.