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Volumn 45, Issue 5, 1999, Pages 619-624

Reverse transcription-competitive multiplex PCR improves quantification of mRNA in clinical samples - Application to the low abundance CFTR mRNA

Author keywords

[No Author keywords available]

Indexed keywords

TRANSMEMBRANE CONDUCTANCE REGULATOR;

EID: 0032897467     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/45.5.619     Document Type: Article
Times cited : (20)

References (20)
  • 1
    • 0026043883 scopus 로고
    • Expression of the cystic fibrosis transmembrane conductance regulator gene in the respiratory tract of normal individuals and individuals with cystic fibrosis
    • 1. Trapnell BC, Chu C-S, Paakko PK, Banks TC, Yoshimura K, Ferrans VJ, et al. Expression of the cystic fibrosis transmembrane conductance regulator gene in the respiratory tract of normal individuals and individuals with cystic fibrosis. Proc Natl Acad Sci U S A 1991;88:6565-9.
    • (1991) Proc Natl Acad Sci U S A , vol.88 , pp. 6565-6569
    • Trapnell, B.C.1    Chu, C.-S.2    Paakko, P.K.3    Banks, T.C.4    Yoshimura, K.5    Ferrans, V.J.6
  • 2
    • 0024828121 scopus 로고
    • Quantitation of mRNA by the polymerase chain reaction
    • 2. Wang AM, Doyle MV, Mark DF. Quantitation of mRNA by the polymerase chain reaction. Proc Natl Acad Sci U S A 1989;86:9717-21.
    • (1989) Proc Natl Acad Sci U S A , vol.86 , pp. 9717-9721
    • Wang, A.M.1    Doyle, M.V.2    Mark, D.F.3
  • 3
    • 0028825308 scopus 로고
    • Methods and reagents. Quantitative PCR: An accurate measure of mRNA?
    • 3. Hengen PN. Methods and reagents. Quantitative PCR: an accurate measure of mRNA? Trends Biochem Sci 1995;20:476-7.
    • (1995) Trends Biochem Sci , vol.20 , pp. 476-477
    • Hengen, P.N.1
  • 4
    • 0028151418 scopus 로고
    • Monitoring mRNA expression by polymerase chain reaction: The "primer dropping" method
    • 4. Wong H, Anderson WD, Cheng T, Riabowol KT. Monitoring mRNA expression by polymerase chain reaction: the "primer dropping" method. Anal Biochem 1994;223:251-8.
    • (1994) Anal Biochem , vol.223 , pp. 251-258
    • Wong, H.1    Anderson, W.D.2    Cheng, T.3    Riabowol, K.T.4
  • 5
    • 0024352638 scopus 로고
    • Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a PCR aided transcript titration assay (PATTY)
    • 5. Becker-André M, Hahlbrock K. Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a PCR aided transcript titration assay (PATTY). Nucleic Acids Res 1989; 17:9437-46.
    • (1989) Nucleic Acids Res , vol.17 , pp. 9437-9446
    • Becker-André, M.1    Hahlbrock, K.2
  • 6
    • 0025355882 scopus 로고
    • Analysis of cytokine mRNA and DNA: Detection and quantitation by competitive polymerase chain reaction
    • 6. Gilliland G, Perrin S, Blanchard K, Bunn HF. Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc Natl Acad Sci U S A 1990;87:2725-9.
    • (1990) Proc Natl Acad Sci U S A , vol.87 , pp. 2725-2729
    • Gilliland, G.1    Perrin, S.2    Blanchard, K.3    Bunn, H.F.4
  • 9
    • 0027943174 scopus 로고
    • An improved method for absolute quantification of mRNA using multiplex polymerase chain reaction: Determination of renin and angiotensin mRNA levels in various tissues
    • 9. Dostal DE, Rothblum KN, Baker KM. An improved method for absolute quantification of mRNA using multiplex polymerase chain reaction: determination of renin and angiotensin mRNA levels in various tissues. Anal Biochem 1994;223:239-50.
    • (1994) Anal Biochem , vol.223 , pp. 239-250
    • Dostal, D.E.1    Rothblum, K.N.2    Baker, K.M.3
  • 10
    • 0027999502 scopus 로고
    • Gene expression analysis by a competitive and differential PCR with antisense competitors
    • 10. de Kant E, Rochlitz CF, Herrmann R. Gene expression analysis by a competitive and differential PCR with antisense competitors. Biotechniques 1994;17:934-42.
    • (1994) Biotechniques , vol.17 , pp. 934-942
    • De Kant, E.1    Rochlitz, C.F.2    Herrmann, R.3
  • 11
  • 12
    • 0027163108 scopus 로고
    • A rapid and versatile method to synthesize internal standards for competitive PCR
    • 12. Celi, FS, Zenilmann ME, Shuldiner AR. A rapid and versatile method to synthesize internal standards for competitive PCR. Nucleic Acids Res 1993;21:1047.
    • (1993) Nucleic Acids Res , vol.21 , pp. 1047
    • Celi, F.S.1    Zenilmann, M.E.2    Shuldiner, A.R.3
  • 13
    • 0027996969 scopus 로고
    • The impact of the PCR plateau phase on quantitative PCR
    • 13. Morrison C, Gannon F. The impact of the PCR plateau phase on quantitative PCR. Biochim Biophys Acta 1994;1219:493-8.
    • (1994) Biochim Biophys Acta , vol.1219 , pp. 493-498
    • Morrison, C.1    Gannon, F.2
  • 14
    • 0027156349 scopus 로고
    • Quantitative titration of nucleic acids by enzymatic amplification reactions run to saturation
    • 14. Pannetier C, Delassus S, Darche S, Saucier C, Kourilsky P. Quantitative titration of nucleic acids by enzymatic amplification reactions run to saturation. Nucleic Acids Res 1993;21:577-83.
    • (1993) Nucleic Acids Res , vol.21 , pp. 577-583
    • Pannetier, C.1    Delassus, S.2    Darche, S.3    Saucier, C.4    Kourilsky, P.5
  • 15
    • 0025014520 scopus 로고
    • Quantitative estimation of minor mRNAs by cDNA-polymerase chain reaction. Application to dystrophin mRNA in cultured myogenic and brain cells
    • 15. Chelly, J, Montarras D, Pinset C, Berwald-Netter Y, Kaplan JC, Kahn A. Quantitative estimation of minor mRNAs by cDNA-polymerase chain reaction. Application to dystrophin mRNA in cultured myogenic and brain cells. Eur J Biochem 1990;187:691-8.
    • (1990) Eur J Biochem , vol.187 , pp. 691-698
    • Chelly, J.1    Montarras, D.2    Pinset, C.3    Berwald-Netter, Y.4    Kaplan, J.C.5    Kahn, A.6
  • 16
    • 0028900860 scopus 로고
    • An evaluation of competitor type and size for use in the determination of mRNA by competitive PCR
    • 16. McCulloch RK, Choong CS, Hurley DM. An evaluation of competitor type and size for use in the determination of mRNA by competitive PCR. PCR Methods Appl 1995;4:219-26.
    • (1995) PCR Methods Appl , vol.4 , pp. 219-226
    • McCulloch, R.K.1    Choong, C.S.2    Hurley, D.M.3
  • 17
    • 0031568947 scopus 로고    scopus 로고
    • The use of the reverse transcription-competitive polymerase chain reaction to investigate the in vivo regulation of gene expression in small tissue samples
    • 17. Auboeuf D, Vidal H. The use of the reverse transcription-competitive polymerase chain reaction to investigate the in vivo regulation of gene expression in small tissue samples. Anal Biochem 1997;245:141-8.
    • (1997) Anal Biochem , vol.245 , pp. 141-148
    • Auboeuf, D.1    Vidal, H.2
  • 18
    • 0026503339 scopus 로고
    • A sensitive viral capture assay for detection of plasma viremia in HIV-infected individuals
    • 18. Henrard DR, Mehaffey WF, Allain JP. A sensitive viral capture assay for detection of plasma viremia in HIV-infected individuals. AIDS Res Hum Retrovir 1992;8:47-52.
    • (1992) AIDS Res Hum Retrovir , vol.8 , pp. 47-52
    • Henrard, D.R.1    Mehaffey, W.F.2    Allain, J.P.3
  • 19
    • 0025124532 scopus 로고
    • Human immunodeficiency virus-infected individuals contain provirus in small numbers of peripheral mononuclear cells and at low copy numbers
    • 19. Simmonds P, Balfe P, Peutherer JF, Ludlam CA, Bishop JO, Brown AJ. Human immunodeficiency virus-infected individuals contain provirus in small numbers of peripheral mononuclear cells and at low copy numbers. J Virol 1990;64:864-72.
    • (1990) J Virol , vol.64 , pp. 864-872
    • Simmonds, P.1    Balfe, P.2    Peutherer, J.F.3    Ludlam, C.A.4    Bishop, J.O.5    Brown, A.J.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.