Organotypic keratinocyte cocultures in defined medium with regular epidermal morphogenesis and differentiation

Journal of Investigative Dermatology

Volumn 112, Issue 5, 1999, Pages 681-691

  Stark, Hans Jürgen ^a   Baur, Markus ^a,b   Breitkreutz, Dirk ^a   Mirancea, Nicolae ^a,c   Fusenig, Norbert E ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^b NESTLÉ RESEARCH CENTER   (Switzerland)

^c INSTITUTE OF BIOLOGY   (Romania)

Author keywords

Epithelial proliferation; Fibroblast dynamics; Serum free cultivation; Skin equivalents

Indexed keywords

KERATIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; EPIDERMIS; FIBROBLAST; HUMAN; HUMAN CELL; KERATINOCYTE; LIPID METABOLISM; MORPHOGENESIS; MOUSE; NONHUMAN; NUDE MOUSE; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; ANTIGENS, DIFFERENTIATION; BASEMENT MEMBRANE; CELL DIFFERENTIATION; CELL DIVISION; COCULTURE TECHNIQUES; CULTURE MEDIA, SERUM-FREE; EPIDERMIS; FIBROBLASTS; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; KERATINOCYTES; MICE; MICE, NUDE; MICROSCOPY, ELECTRON; PHENOTYPE;

EID: 0032896837     PISSN: 0022202X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1523-1747.1999.00573.x     Document Type: Article

References (61)

1. Asselineau D, Bernard BA, Bailly C, Darmon M: Retinoic acid improves epidermal morphogenesis. Dev Biol 133:322-335, 1989
2. Baur M: Charakterisierung von Proliferation und Differenzierung humaner Keratinozyten mit unterschiedlichem Transformationsgrad unter definierten Mediumbedingungen: Effekte mesenchymaler Zellen und modulierender Faktoren. PhD Thesis, University of Hohenheim, Germany, 1995:1-172
3. Bell E, Ehrlich HP, Buttle DJ, Nakatsuji T: Living tissue formed in vitro and accepted as skin-equivalent tissue of full thickness. Science 211:1052-1054, 1981
4. Boukamp P, Breitkreutz D, Stark HJ, Fusenig NE: Mesenchyme-mediated and endogenous regulation of growth and differentiation of human skin keratinocytes derived from different body sites. Differentiation 44:150-161, 1990
5. Boxman I, Löwik C, Aarden L, Ponec M: Modulation of IL-6 production and IL-1 activity by keratinocyte-fibroblast interaction. J Invest Dermatol 101:316-324, 1993
6. Boyce ST: Cultured skin substitutes: a review. Tissue Eng 2:255-266, 1996
7. Boyce ST, Ham RG: Calcium regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture. J Invest Dermatol 81:33s-40s, 1983
8. Boyce ST, Williams ML: Lipid supplemented medium induces lamellar bodies and precursors of barrier lipids in cultured analogues of human skin. J Invest Dermatol 101:180-184, 1993
9. 2+. Differentiation 54:201-217, 1993
10. Breitkreutz D, Stark HJ, Mirancea N, Tomakidi P, Steinbauer H, Fusenig NE: Integrin and basement membrane normalization in mouse grafts of human keratinocytes - implications for epidermal homeostasis. Differentiation 61:195-209, 1997
11. Chen C-SJ, Lavker RM, Rodeck U, Risse B, Jensen PJ: Use of a serum-free epidermal culture model to show deleterious effects of epidermal growth factor on morphogenesis and differentiation. J Invest Dermatol 104:107-112, 1995
12. Chen YQ, Mauviel A, Ryynanen J, Sollberg S, Uitto J: Type VII collagen gene expression by human skin fibroblasts and keratinocytes in culture: influence of donor age and cytokine responses. J Invest Dermatol 102:205-209, 1994
13. Choi Y, Fuchs E: TGFβ and retinoic acid: regulators of growth and modifiers of differentiation in human epidermal cells. Cell Regulation 1:791-809, 1990
14. Contard P, Bartel RL, Jacobs HL, et al: Culturing keratinocytes and fibroblasts in a three-dimensional mesh results in epidermal differentiation and formation of a basal lamina-anchoring zone. J Invest Dermatol 100:35-39, 1993
15. Coulomb B, Lebreton C, Dubertet L: Influence of human dermal fibroblasts on epidermalization. J Invest Dermatol 92:122-125, 1989
16. Fartasch M, Ponec M: Improved barrier structure formation in air-exposed human culture systems. J Invest Dermatol 102:366-374, 1994
17. Fleischmajer R, Contard P, Schwartz E, MacDonald HED, Jacobs HL, Sakai LY: Elastin associated microfibrils (10 nm) in a three-dimensional fibroblast culture. J Invest Dermatol 97:638-643, 1991
18. Foidart JM, Reddi AH: Immunofluorescent localization of type IV collagen and laminin during enchondral bone differentiation and regulation by pituitary growth hormone. Dev Biol 75:130-136, 1980
19. Fulbright RM, Axelrod D, Dunham WR, Marcelo CL: Fatty acid alteration and the lateral diffusion of lipids in the plasma membrane of keratinocytes. Exp Cell Res 233:128-134, 1997
20. Fusenig NE: Epithelial-mesenchymal interactions regulate keratinocyte growth and differentiation in vitro. In: Leigh I, Lane B, Watt F (eds) The Keratinocyte Handbook. Cambridge: Cambridge University Press, 1994a, pp. 71-94
21. Fusenig NE: Cell culture models: Reliable tools in pharmacotoxicology? In: Fusenig NE, Graf H (eds). Cell Culture in Pharmaceutical Research. Berlin: Springer-Verlag, 1994b, pp. 1-7
22. Garlick JA, Taichman LB: Fate of human keratinocytes during reepithelialization in an organotypic culture model. Lab Invest 70:916-924, 1994
23. Gay R, Swiderek M, Nelson D, Ernesti A: The living skin equivalent as a model in vitro for ranking the toxic potential of dermal irritants. Toxic In Vitro 6:303-315, 1992
24. Gibbs S, Vicanová J, Bouwstra J, Valstar D, Kempenaar J, Ponec M: Culture of reconstructed epidermis in a defined medium at 33°C shows a delayed epidermal maturation, prolonged lifespan and improved stratum corneum. Arch Dermatol Res 289:585-595, 1997
25. Green H: Cyclic AMP in relation to proliferation of the epidermal cell: a new view. Cell 15:801-811, 1978
26. Green S: Promiscuous liasons. Nature 361:590-591, 1993
27. Hanley K, Jiang Y, Crumrine D, et al: Activators of the nuclear hormone receptors PPARα and FXR accelerate the development of the fetal epidermal permeability barrier. J Clin Invest 100:705-712, 1997
28. Hohl D, Mehrel T, Lichti U, Turner ML, Roop DR, Steinert PM: Characterization of human loricrin. J Biol Chem 266:6626-6636, 1991
29. Johnson EW, Meunier SF, Roy CJ, Parenteau NL: Serial cultivation of normal human keratinocytes: a defined system for studying the regulation of growth and differentiation. In Vitro Cell Dev Biol 28A:429-435, 1992
30. Lambert CA, Soudant EP, Nusgens BV, Lapière CM: Pretranslational regulation of extracellular matrix macromolecules and collagenase expression in fibroblasts by mechanical forces. Lab Invest 66:444-451, 1992
31. Leigh IM, Navsaria H, Purkis PEMC, Kay IA, Bowden PE, Riddle PN: Keratins (K16 and K17) as markers of keratinocyte hyperproliferation in psoriasis in vivo and in vitro. Br J Dermatol 133:501-511, 1995
32. Limat A, Hunziker T, Boillat C, Bayreuther K, Noser F: Post-mitotic human dermal fibroblasts efficiently support the growth of human follicular keratinocytes. J Invest Dermatol 92:758-762, 1989
33. Limat A, Mauri D, Hunziker T: Successful treatment of chronic leg ulcers with epidermal equivalents generated from cultured autologous outer root sheath cells. J Invest Dermatol 107:128-135, 1996
34. Meier CA: Regulation of gene expression by nuclear hormone receptors. J Recept Signal Transduct Res 17:319-335, 1997
35. Michel M, Auger FA, Germain L: Anchored skin equivalent cultured in vitro: a new tool for percutaneous absorption studies. In Vitro Cell Dev Biol 29A:834-837, 1993
36. Noser FK, Limat A: Organotypic culture of outer root sheath cells from human hair follicles using a new culture device. In Vitro Cell Dev Biol 23:541-545, 1987
37. Nuss Parker J, Zhao W, Askins KJ, Broker TR, Chow LT: Mutational analyses of differentiation-dependent human papillomavirus type 18 enhancer elements in epithelial raft cultures of neonatal foreskin keratinocytes. Cell Growth Differ 8:751-762, 1997
38. Parenteau NL, Nolte CM, Bilbo P, et al: Epidermis generated in vitro: Practical considerations and applications. J Cell Biochem 45:245-251, 1991
39. Paye M, Nusgens BV, Lapière CM: Modulation of cellular biosynthetic activity in the retracting collagen lattice. Eur J Cell Biol 45:44-50, 1987
40. Pittelkow MR, Scott RE: New techniques for the in vitro culture of human skin keratinocytes and perspectives on their use for grafting of patients with extensive burns. Mayo Clin Proc 61:771-777, 1986
41. Ponec M, Kempenaar J: Use of human skin recombinants as an in vitro model for testing the irritation potential for cutaneous irritants. Skin Pharmacol 8:49-59, 1995
42. Ponec M, Kempenaar J, de Weerheim A, Lannoy L, Kalkman I, Jansen H: Triglyceride metabolism in human keratinocytes cultured at the air-liquid interface. Arch Dermatol Res 287:723-730, 1995
43. Ponec M, Gibbs S, Weerheim A, Kempenaar J, Mulder A, Mommaas AM: Epidermal growth factor and temperature regulate keratinocyte differentiation. Arch Dermatol Res 289:317-326, 1997a
44. Ponec M, Weerheim A, Kempenaar J, Mulder A, Gooris GS, Bouwstra J, Mommaas AM: The formation of competent barrier lipids in reconstructed human epidermis requires the presence of vitamin C. J Invest Dermatol 109:348-355, 1997b
45. Regnier M, Asselineau D, Lenoir MC: Human epidermis reconstructed on dermal substrates in vitro: an alternative to animals in skin pharmacology. Skin Pharmacol 3:70-85, 1990
46. Regnier M, Darmon M: 1,25-dihydroxyvitamin D3 stimulates specifically the last steps of epidermal differentiation of cultured human keratinocytes. Differentiation 47:173-188, 1991
47. Rikimaru K, Molès JP, Watt FM: Correlation between hyperproliferation and suprabasal integrin expression in human epidermis reconstituted in culture. Exp Dermtol 6:214-221, 1997
48. Roop D: Defects in the barrier. Science 267:474-475, 1995
49. Roop D, Cheng CK, Titterington L, Meyers A, Stanley JR, Steinert PM, Yuspa SH: Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984
50. Rosdy M, Clauss LC: Terminal epidermal differentiation of human keratinocytes grown in chemically defined medium on inert filter substrates at the air-liquid interface. J Invest Dermatol 95:409-414, 1990
51. Sarber R, Hull B, Merill C, Soranno T, Bell E: Regulation of proliferation of fibroblasts of low and high population doubling levels grown in collagen lattices. Mech Ageing Dev 17:107-117, 1981
52. Slivka SR, Landeen LK, Zeigler F, Zimber MP, Bartel RL: Characterization, barrier function, and drug metabolism of an in vitro skin model. J Invest Dermatol 100:40-46, 1993
53. Smola H, Thiekötter G, Fusenig NE: Mutual induction of growth factor gene expression by epidermal-dermal cell interaction. J Cell Biol 122:417-429, 1993
54. Smola H, Thiekötter G, Baur M, Stark HJ, Breitkreutz D, Fusenig NE: Organotypic and epidermal-dermal cocultures of normal human keratinocytes and dermal cells: Regulation of transforming growth factor α, β1 and β2 mRNA levels, Toxic In Vitro 8:641-650, 1994
55. Smola H, Stark HJ, Thiekötter G, Mirancea N, Krieg T, Fusenig NE: Dynamics of basement membrane formation by keratinocyte-fibroblast interactions in organotypic skin culture. Exp Cell Res 239:399-410, 1998
56. Vicanová J, Mommaas AM, Mulder AA, Koerten HK, Poner M: Impaired desquamation in the in vitro reconstructed human epidermis. Cell Tissue Res 286:115-122, 1996
57. Watt FM: Terminal differentiation of epidermal keratinocytes. Curr Opin Cell Biol 1:1107-115, 1989
58. Watt FM, Boukamp P, Hornung J, Fusenig NE: Effect of growth environment on spatial expression of involucrin by human epidermal keratinocytes. Arch Dermatol Res 279:335-340, 1987
59. Werner S, Peters KG, Longaker MT, Fuller-Pace F, Banda MJ, Williams LT: Large induction of keratinocyte growth factor expression in the dermis during wound healing. Prof Natl Acad Sci USA 98:6896-6900, 1992
60. Wu YJ, Parker LM, Binder NE, Beckett MA, Sinard JH, Griffiths CT: The mesothelial keratins: a new family of cytoskeletal proteins identified in cultured mesothelial cells and nonkeratinizing epithelia. Cell 31:693-703, 1982
61. Zambruno G, Marchisio PC, Marconi A, Vaschierei C, Melchiori A, Gianetti A, DeLuca M: Transforming growth factor β1 modulates β1 and β5 integrin receptors and induces the de novo expression of the αvβ6 heterodimer in normal human keratinocytes: implications for wound healing. J Cell Biol 129:853-866, 1995