Rat liver endothelial cell glutamine transporter and glutaminase expression contrast with parenchymal cells

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 276, Issue 3 39-3, 1999, Pages

  Lohmann, Rüdiger ^a   Souba, Wiley W ^a   Bode, Barrie P ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Amino acid transport; ATB0; System ASC; System B0; System N

Indexed keywords

GLUTAMINE;

AMINO ACID METABOLISM; AMINO ACID TRANSPORT; ANIMAL CELL; ARTICLE; CELL TYPE; CONTROLLED STUDY; ENDOTHELIUM CELL; GENE EXPRESSION; HOMEOSTASIS; LIVER PARENCHYMA; LIVER SINUSOID; MALE; METABOLIC REGULATION; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REGULATORY MECHANISM;

EID: 0032896832     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.1999.276.3.g743     Document Type: Article

References (45)

1. Arriza, J. L., M. P. Kavanaugh, W. A. Fairman, Y. N. Wu, G. H. Murdoch, R. A. North, and S. G. Amara. Cloning and expression of a human neutral amino acid transporter with structural similarity to the glutamate transporter gene family. J. Biol. Chem. 268: 15329-15332, 1993.
2. Austgen, T. R., M. K. Chen, T. C. Flynn, and W. W. Souba. The effects of endotoxin on the splanchnic metabolism of glutamine and related substrates. J. Trauma 31: 742-52, 1991.
3. Bioulac-Sage, P., B. LeBail, and C. Balabaud. Liver and biliary tract histology. In: Oxford Textbook of Clinical Hepatology, edited by N. McIntyre, J. P. Benhamou, J. Bircher, M. Rizzetto and J. Rodes. New York: Oxford University Press, 1991, p. 12-20.
4. Bissell, D. M., L. Hammaker, and R. Schmid. Liver sinusoidal cells: identification of a subpopulation for erythrocyte catabolism. J. Cell Biol. 54: 107-119, 1972.
5. Bode, B. P., D. L. Kaminski, W. W. Souba, and A. P. Li. Glutamine transport in isolated human hepatocytes and transformed liver cells. Hepatology 21: 511-520, 1995.
6. Bode, B. P., N. Reuter, J. L. Conroy, and W. W. Souba. Protein kinase C regulates nutrient uptake and growth in hepatoma cells. Surgery 124: 260-268, 1998.
7. Bode, B., B. K. Tamarappoo, M. Mailliard, and M. S. Kilberg. Characteristics and regulation of hepatic glutamine transport. JPEN J. Parenter. Enteral Nutr. 14: 51S-55S, 1990.
8. Bouwens, L., P. De Bleser, K. Vanderkerken, B. Geerts, and E. Wisse. Liver cell heterogeneity: functions of non-parenchymal cells. Enzymes 46: 155-168, 1992.
9. Burns, D. M., B. Bhandari, J. M. Short, P. G. Sanders, R. H. Wilson, and R. E. Miller. Selection of a rat glutamine synthetase cDNA clone. Biochem. Biophys. Res. Commun. 134: 146-151, 1986.
10. Bussolati, O., R. Sala, A. Astorri, B. M. Rotoli, V. Dall'Asta, and G. C. Gazzola. Characterization of amino acid transport in human endothelial cells. Am. J. Physiol. 265 (Cell Physiol. 34): C1006-C1014, 1993.
11. Chomczynski, P., and N. Sacchi. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156-159, 1987.
12. Dall'Asta, V., P. A. Rossi, O. Bussolati, G. G. Guidotti, and G. C. Gazzola. The transport of L-glutamine into cultured human fibroblasts. Biochim. Biophys. Acta 1052: 106-112, 1990.
13. Darmaun, D., D. E. Matthews, and D. M. Bier. Glutamine and glutamate kinetics in humans. Am. J. Physiol. 251 (Endocrinol. Metab. 14): E117-E126, 1986.
14. Darmaun, D., D. E. Matthews, J. F. Desjeux, and D. M. Bier. Glutamine and glutamate nitrogen exchangeable pools in cultured fibroblasts: a stable isotope study. J. Cell. Physiol. 134: 143-148, 1988.
15. De Leeuw, A. M., A. Brouwer, and D. L. Knook. Sinusoidal endothelial cells of the liver: fine structure and function in relation to age. J. Electron Microsc. (Tokyo) 14: 218-236, 1990.
16. 0) similar to that in bovine renal brush border membrane vesicles. Biochim. Biophys. Acta 1104: 55-62, 1992.
17. Fischer, C. P., B. P. Bode, and W. W. Souba. Starvation and endotoxin act independently and synergistically to coordinate hepatic glutamine transport. J. Trauma 40: 688-693, 1996.
18. Friedman, S. L., and F. J. Roll. Isolation and culture of hepatic lipocytes, Kupffer cells, and sinusoidal endothelial cells by density gradient centrifugation with Stractan. Anal. Biochem. 161: 207-18, 1987.
19. Gazzola, G. C., V. Dall'Asta, R. Franchi-Gazzola, and M. F. White. The cluster-tray method for rapid measurement of solute fluxes in adherent cultured cells. Anal. Biochem. 115: 368-374, 1981.
20. Haussinger, D. Liver glutamine metabolism. J. Parenter. Enteral Nutr. 14: 56S-62S, 1990.
21. Haussinger, D., H. Sies, and W. Gerok. Functional hepatocyte heterogeneity in ammonia metabolism. The intercellular glutamine cycle. J. Hepatol. 1: 3-14, 1985.
22. Haussinger, D., S. Soboll, A. J. Meijer, W. Gerok, J. M. Tager, and H. Sies. Role of plasma membrane transport in hepatic glutamine metabolism. Eur. J. Biochem. 152: 597-603, 1985.
23. Haussinger, D., T. Stehle, and W. Gerok. Glutamine metabolism in isolated perfused rat liver. The transamination pathway. Biol. Chem. 366: 527-536, 1985.
24. Hediger, M. A., Y. Kanai, G. You, and S. Nussberger. Mammalian ion-coupled solute transporters. J. Physiol. (Lond.) 482: 7S-17S, 1995.
25. Herskowitz, K., B. P. Bode, E. R. Block, and W. W. Souba. Characterization of L-glutamine transport by pulmonary artery endothelial cells. Am. J. Physiol. 260 (Lung Cell. Mol. Physiol. 4): L241-L246, 1991.
26. Irving, M. G., F. J. Roll, S. Huang, and D. M. Bissell. Characterization and culture of sinusoidal endothelium from normal rat liver: lipoprotein uptake and collagen phenotype. Gastroenterology 87: 1233-1247, 1984.
27. Kekuda, R., P. D. Prasad, Y. J. Fei, V. Torres-Zamorano, S. Sinha, T. L. Yang-Feng, F. H. Leibach, and V. Ganapathy. Cloning of the sodium-dependent, broad-scope, neutral amino acid transporter Bo from a human placental choriocarcinoma cell line. J. Biol. Chem. 271: 18657-18661, 1996.
28. 0. Am. J. Physiol. 272 (Gastrointest. Liver Physiol. 35): G1463-G1472, 1997.
29. Kilberg, M. S., M. E. Handlogten, and H. N. Christensen. Characteristics of an amino acid transport system in rat liver for glutamine, asparagine, histidine, and closely related analogs. J. Biol. Chem. 255: 4011-4019, 1980.
30. Knook, D. L., N. Blansjaar, and E. C. Sleyster. Isolation and characterization of Kupffer and endothelial cells from the rat liver. Exp. Cell Res. 109: 317-329, 1977.
31. Knook, D. L., and E. C. Sleyster. Separation of Kupffer and endothelial cells of the rat liver by centrifugal elutriation. Exp. Cell Res. 99: 444-449, 1976.
32. Low, S. Y., M. Salter, R. G. Knowles, C. I. Pogson, and M. J. Rennie. A quantitative analysis of the control of glutamine catabolism in rat liver cells. Use of selective inhibitors. Biochem. J. 295: 617-624, 1993.
33. Low, S. Y., P. M. Taylor, H. S. Hundal, C. I. Pogson, and M. J. Rennie. Transport of L-glutamine and L-glutamate across sinusoidal membranes of rat liver. Effects of starvation, diabetes and corticosteroid treatment. Biochem. J. 284: 333-340, 1992.
34. Nagelkerke, J. F., K. P. Barto, and T. J. C. van Berkel. In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer and parenchymal cells. J. Biol. Chem. 258: 12221-12227, 1983.
35. Pan, M., M. Wasa, U. Ryan, and W. Souba. Inhibition of pulmonary microvascular endothelial glutamine transport by glucocorticoids and endotoxin. JPEN J. Parenter. Enteral Nutr. 19: 477-481, 1995.
36. Pitas, R. E., J. Boyles, R. W. Mahley, and D. M. Bissell. Uptake of chemically modified low density lipoproteins in vivo is mediated by specific endothelial cells. J. Cell Biol. 100: 103-117, 1985.
37. +/glutamate cotransporters. J. Biol. Chem. 268: 15351-15355, 1993.
38. Shapiro, R. A., L. Farrell, M. Srinivasan, and N. P. Curthoys. Isolation, characterization, and in vitro expression of a cDNA that encodes the kidney isoenzyme of mitochondrial glutaminase. J. Biol. Chem. 266: 18792-18796, 1991.
39. Smith, E. M., and M. Watford. Molecular cloning of a cDNA for rat hepatic glutaminase: sequence similarity to kidney-type glutaminase. J. Biol. Chem. 265: 10631-10636, 1990.
40. Spolarics, Z. Endotoxin stimulates gene expression of ROS-eliminating pathways in rat hepatic endothelial and Kupffer cells. Am. J. Physiol. 270 (Gastrointest. Liver Physiol. 33): G660-G666, 1996.
41. Spolarics, Z., C. H. Lang, G. J. Bagby, and J. J. Spitzer. Glutamine and fatty acid oxidation are the main sources of energy for Kupffer and endothelial cells. Am. J. Physiol. 261 (Gastrointest. Liver Physiol. 24): G185-G190, 1991.
42. 2 detoxification in LPS-activated hepatic endothelial and Kupffer cells. Am. J. Physiol. 273 (Gastrointest. Liver Physiol. 36): G1304-G1311, 1997.
43. +-dependent neutral amino acid transporter. J. Biol. Chem. 271: 14883-14890, 1996.
44. Watford, M. Hepatic glutaminase expression: relationship to kidney-type glutaminase and to the urea cycle. FASEB J. 7: 1468-1474, 1993.
45. Wisse, E., J. van der Meulen, and J. M. Noordende. Observations of the fine structure and peroxidase cytochemistry of normal rat liver Kupffer cells. J. Ultrastruct. Res. 46: 393-426, 1974.