Dexamethasone induces apoptosis in human T cell clones expressing low levels of Bcl-2

Cell Death and Differentiation

Volumn 6, Issue 1, 1999, Pages 79-86

  Gilardini Montani, Maria Saveria ^a,d   Tuosto, L ^b   Giliberti, R ^b   Stefanini, L ^b   Cundari, E ^c   Piccolella, E ^b  

^a UNIVERSITY OF TUSCIA   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

^c CNR   (Italy)

^d Largo dell'Univ 01100 Viterbo I   (Italy)

Author keywords

Apoptosis; Bcl 2; Dex; Human T lymphocytes; IL 2

Indexed keywords

DEXAMETHASONE; FAS ANTIGEN; INTERLEUKIN 2; PROTEIN BAX; PROTEIN BCL 2; PROTEIN BCL X;

APOPTOSIS; ARTICLE; CELL CYCLE G0 PHASE; CELL CYCLE G1 PHASE; CONTROLLED STUDY; HUMAN; HUMAN CELL; LYMPHOCYTE CLONE; LYMPHOCYTE PROLIFERATION; PHENOTYPE; PRIORITY JOURNAL; T LYMPHOCYTE;

EID: 0032892384     PISSN: 13509047     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cdd.4400461     Document Type: Article

References (49)

1. Kappler JW, Roehm N and Marrack P (1987) T cell tolerance by clonal elimination in the thymus. Cell 49: 273-280
2. Rocha B, Tanchot C and Von Bohemer H (1993) Clonal anergy blocks in vivo growth of mature T cells and can be reversed in the absence of antigen. J. Exp. Med. 177: 1517-1521
3. King LB and Ashwell JD (1993) Signaling for death of lymphoid cells. Curr. Opin. Immunol. 5: 368-373
4. Kroemer G and Martinez C (1994) Pharmacological inhibition of programmed lymphocyte death. Immunol. Today 15: 235-242
5. Gilardini Montani MS, Tuosto L and Piccolella E (1996) Apoptos s: prospects for pharmacological manipulation of activated programmed cell death in immunological disease. Clin. Immunother. 5: 413-419
6. Alnemri ES and Litwack G (1989) Glucocorticoid-induced lymphocytolysis is not mediated by an induced endonuclease. J. Biol. Chem. 264: 4104-4111
7. Cohen JJ and Duke RC (1984) Glucocorticoid activation of a calcium-dependent endonuclease in thymocyte nuclei leads to cell death. J. Immunol. 132: 38-42
8. Gonzalo JA, Gonzales-Garcia A, Martinez C and Kroemer G (1993) Glucocorticoid-mediated control of the activation and clonal deletion of peripheral T cells in vivo. J. Exp. Med. 177: 1239-1246
9. Nagata S and Golstein P (1995) The Fas death factor. Science 267: 1449-1456
10. Lynch DH, Ramsdell F and Alderson MR (1995) Fas and FasL in the homeostatic regulation of immune responses. Immunol. Today 16: 569-574
11. Korsmeyer SJ (1992) Bcl-2: a repressor of lymphocyte death. Immunol. Today 13: 285-288
12. Yang E and Korsmeyer SJ (1996) Molecular thanatopsis: a disccurse on the Bcl-2 family and cell death. Blood 88: 386-401
13. Ohem A, Behrmann I, Falk W, Pawlita M, Maier G, Klast C, Li-Weber M, Richards S, Dhein J, Trauth BC, Ponsting H and Krammer P (1992) Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. J. Biol. Chem. 267: 10709-10715
14. Yonehara S, Ishii AI and Yonehara M (1989) A cell-killing monoclonal antibody (anti-Fas) to a cell surface antigen co-downregulated with the receptor of tumor necrosis factor. J. Exp. Med. 169: 1747-1756
15. Takahashi T, Tanaka M, Inazawa J, Abe T, Suda T and Nagata S (1994) Human Fas ligand: gene structure, chromosomal location and species specificity. Int. Immunol. 6: 1567-1574
16. Alderson MR, Tough TW, Davis-Smith T, Braddy S, Falk B, Schooley KA, Goodwin RG, Smith CA, Ramsdell F and Lynch DH (1995) Fas ligand mediates activation-induced cell death in human T lymphocytes. J. Exp. Med. 181: 71-77
17. Hockenbery D, Nunez G, Milliman C, Schreiber RD and Korsmeyer SJ (1990) Bcl-2 is a inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334-336
18. Krajewski S, Tanaka S, Takayama S, Schibler MJ, Fenton W and Reed JC (1994) Investigation of the subcellular distribution of the Bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum and outer mithocondrial membranes. Cancer Res. 53: 4701-4714
19. Yin X-M, Oltvai ZN and Korsmeyer SJ (1994) BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax. Nature 369: 321-323
20. Oltvai ZN, Milliman CL and Korsmeyer SJ (1993) Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74: 609-619
21. Boise LH, Gonzales-Garcia M, Postema CE, Ding L, Lindsten T, Turka LA, Mao X. Nunez G and Thompson CB (1993) Bcl-x, a Bcl-2-related gene that functions as a dominant regulator of apoptotic cell death. Cell 74: 597-608
22. L and Bcl-2 repress a common pathway of cell death. J. Exp. Med. 182: 821-828
23. Van Parijs L and Abbas AK (1996a) Role of Fas-mediated cell death in the regulation of immune response. Curr. Opin. Immunol. 8: 355-361
24. Van Parijs L, Ibraghimov A and Abbas AK (1996b) The roles of costimulation and Fas in T cell apoptosis and peripheral tolerance. Immunity 4: 321-328
25. Miyazaki T, Liu Z-J, Kawara A, Minami Y, Yamada K, Tsujimoto Y, Barsoumian EL, Permutter RM and Taniguchi T (1995) Three distinct IL-2 signaling pathways mediated by Bcl-2, c-myc and lck cooperate in hematopoietic cell proliferation. Cell 81: 223-231
26. Ahmed NN, Leighton Grimes H, Bellacosa A, Chan TO and Tsichlis PN (1997) Transduction of interleukin-2 antiapoptotic and proliferative signals via Akt protein kinase. Proc. Natl. Acad. Sci. USA 94: 3627-3632
27. Nieto MA and Lopez-Rivas A (1989) IL-2 protects T lymphocytes from glucocorticoid-induced DNA fragmentation and cell death. J. Immunol. 143: 4166-4170
28. Broome HE, Dargan CM, Bessent EF, Krajewski S and Reed JC (1995) Apoptosis and Bcl-2 expression in cultured murine splenic T cells. Immunology 84: 375-382
29. Tuosto L, Cundari E, Gilardini Montani MS and Piccolella E (1994) Analysis of susceptibility of mature human T lymphocytes to dexamethasone-induced apoptosis. Eur. J. Immunol. 24: 1061-1065
30. Reed JC (1994) Bcl-2 and the regulation of programmed cell death. J. Cell Biol. 124: 1-6
31. Sentman CL, Shutter JR, Hochenbery D, Kanagawa O and Korsmeyer SJ (1991) Bcl-2 inhibits multiple forms of apoptosis but not negative selection in thymocytes. Cell 67: 879-888
32. Strasser A, Harris AW and Cory S (1991) Bcl-2 transgene inhibits T cell death and perturbs thymic self-censorship. Cell 67: 889-899
33. Siegel RM, Katsumata M, Miyashita T, Louie DC, Greene MI and Reed JC (1992) Inhibition of thymocyte apoptosis and negative antigenic selection in Bcl-2 transgenic mice. Proc. Natl. Acad. Sci. USA 89: 7003-7007
34. Merino R, Ding L, Veis DJ, Korsmeyer SJ and Nunez G (1994) Developmental regulation of the Bcl-2 protein and susceptibility to cell death in B lymphocytes. EMBO J. 13: 683-691
35. Alnemri ES, Fernandes TF, Haldar S, Croce CM and Litwack G (1992) Involvement of Bcl-2 in glucocorticoid-induced apoptosis of human pre-B-leukemias. Cancer Res. 52: 491-495
36. Miyashita T and Reed JC (1992) Bcl-2 gene transfer increases relative resistance of S49.1 and WEHI7.2 lymphoid cells to cell death and DNA fragmentation induced by glucocorticoids and multiple chemotherapeutic drugs. Cancer Res. 52: 5407-5411
37. Moreno MB, Memon SA and Zacharchuk CM (1996) Apoptosis signaling pathways in normal T cells. Differential activity of Bcl-2 and IL-1 b-converting enzyme family protease inhibitors on glucocorticoid- and Fas-mediated cytotoxicity. J. Immunol. 157: 3845-3849
38. Deng G and Podack ER (1993) Suppression of apoptosis in a cytotoxic T-cell line by interleukin 2-mediated gene transcription and deregulated expression of the protooncogene Bcl-2. Proc. Natl. Acad. Sci. USA 90: 2189-2193
39. Zubiaga AM, Munoz E and Huber BT (1992) IL-4 and IL-2 selectively rescue Th cell subsets from glucocorticoid-induced apoptosis. J. Immunol. 149: 107-112
40. Mor F and Cohen IR (1996) IL-2 rescues antigen-specific T cells from radiation or dexamethasone-induced apoptosis: Correlation with induction of Bcl-2. J. Immunol. 156: 515-522
41. Taniguchi T and Minami Y (1993) The IL-2/IL-2 receptor system: a current overview. Cell 73: 5-8
42. Paliogianni F, Ahuja SS, Balow JP, Balow JE and Boumpas DT (1993) Novel mechanism for inhibition of human T cells by glucocorticoids. Glucocorticoids inhibit signal transduction through IL-2 receptor. J. Immunol. 151: 4081-4089
43. Paliogianni F, Hama N, Balow JE, Valentine MA and Boumpas DT (1995) Glucocorticoid-mediated regulation of protein phosphorylation in primary human T cells. Evidence for induction of phosphatase activity. J. Immunol. 155: 1809-1817
44. + helper T cells. A model for the long-term survival of memory cells. J. Immunol. 156: 1764-1771
45. Yang Y, Mercep M, Ware CF and Ashwell JD (1995) Fas and activation-induced Fas ligand mediate apoptosis of T cell hybridomas: inhibition of Fas ligand expression by retinoic acid and glucocorticoids. J. Exp. Med. 181: 1673-1682
46. Lombardi G, Sidhu S, Lamb JR, Batchelor JR and Lechler RI (1989) Co-recognition of endogenous antigens with HLA-DR1 by alloreactive human T cell clones. J. Immunol. 142: 753-759
47. Sidhu S, Deacock S, Bal V, Batchelor JR, Lombardi G and Lechler RI (1992) Human T cells cannot act as autonomous antigen-presenting cells, but induce tolerance in antigen-specific and alloreactive responder cells. J. Exp. Med. 176: 875-880
48. Nicoletti I, Migliorati G, Pagliacci MC, Grignani F and Riccardi C (1991) A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J. Immunol. Meth. 139: 271-279
49. Chomczynski P and Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156-159