Thermoregulated expression and characterization of an NAD(P)H-dependent 2-cyclohexen-1-one reductase in the plant pathogenic bacterium Pseudomonas syringae pv. glycinea

Journal of Bacteriology

Volumn 181, Issue 3, 1999, Pages 814-822

  Rohde, Bettina H ^a   Schmid, Roland ^b   Ullrich, Matthias S ^a  

^a MAX PLANCK INSTITUTE FOR TERRESTRIAL MICROBIOLOGY   (Germany)

^b UNIVERSITY OF OSNABRÜCK   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

2 CYCLOHEXENONE; 2 CYCLOHEXENONE REDUCTASE; 3 PENTEN 2 ONE; BACTERIAL ENZYME; FLAVINE MONONUCLEOTIDE; GENE PRODUCT; MORPHINONE; MORPHINONE REDUCTASE; OXIDOREDUCTASE; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME SUBSTRATE; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN EXPRESSION; PSEUDOMONAS PUTIDA; PSEUDOMONAS SYRINGAE; SEQUENCE HOMOLOGY; SOYBEAN; THERMOREGULATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GLYCINE MAX; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS PUTIDA; PSEUDOMONAS SYRINGAE; PSEUDOMONAS SYRINGAE PV. GLYCINEA;

EID: 0032891449     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.3.814-822.1999     Document Type: Article

References (44)

1. Bender, C. L., H. Liyanage, D. Palmer, M. Ullrich, S. Young, and R. Mitchell. 1993. Characterization of the genes controlling biosynthesis of the polyketide phytotoxin coronatine including conjugation between coronafacic and coronamic acid. Gene 133:31-38.
2. Bender, C. L., D. A. Palmer, A. Penaloza-Vazquez, V. Rangaswamy, and M. Ullrich. 1996. Biosynthesis of coronatine, a thermoregulated phytotoxin produced by the phytopathogen Pseudomonas syringae. Arch. Microbiol. 166:71-75.
3. Bender, C. L., S. A. Young, and R. E. Mitchell. 1991. Conservation of plasmid DNA sequences in coronatine-producing pathovars of Pseudomonas syringae. Appl. Environ. Microbiol. 57:993-999.
4. Brace, N. C., C. J. Wilmot, K. N. Jordan, A. E. Trebilcock, L. D. Gray Stephens, and C. R. Lowe. 1990. Microbial degradation of the morphine alkaloids: identification of morphinone as an intermediate in the metabolism of morphine by Pseudomonas putida M10. Arch. Microbiol. 154:465-470.
5. Budde, I. P., B. H. Rohde, C. L. Bender, and M. S. Ullrich. 1998. Growth phase and temperature influence promoter activity, transcript abundance, and protein stability during biosynthesis of the Pseudomonas syringae phytotoxin coronatine. J. Bacteriol. 180:1360-1367.
6. Craig, D. H., P. C. E. Moody, N. C. Bruce, and N. S. Scrutton. 1998. Reductive and oxidative half-reactions of morphinone reductase from Pseudomonas putida M10: a kinetic and thermodynamic analysis. Biochemistry 37:7598-7607.
7. Dempsey, D. A., H. Silva, and D. F. Klessig. 1998. Engineering disease and pest resistance in plants. Trends Microbiol. 6:54-61.
8. Dunleavy, J. M. 1988. Bacterial, fungal, and viral diseases affecting soybean leaves. American Phytopathological Society, St. Paul, Minn.
9. Fox, K. M., and P. A. karplus. 1994. Old yellow enzyme at 2 Å resolution: overall structure, ligand binding, and comparison with related flavoproteins. Structure 2:1089-1105.
10. French, C. E., and N. C. Bruce. 1995. Bacterial morphinone reductase is related to Old Yellow Enzyme. Biochem. J. 312:671-678.
11. French, C. E., and N. C. Bruce. 1994. Purification and characterization of morphinone reductase from Pseudomonas putida M10. Biochem. J. 301:97-103.
12. French, C. E., S. Nicklin, and N. C. Bruce. 1996. Sequence and properties of pentaerythritol tetranitrate reductase from Enterobacter cloacae. J. Bacteriol. 178:6623-6627.
13. Gondry, M., K. H. Diep Le, F. D. Manson, S. K. Chapman, F. S. Mathews, G. A. Reid, and F. Lederer. 1995. On the lack of coordination between protein folding and flavin insertion in Escherichia coli for flavocytochrome b2 mutant forms Y254L and D282N. Protein Sci. 4:925-935.
14. Goss, R. W. 1970. The relation of temperature to common halo blight of beans. Phytopathology 30:258-264.
15. Hettwer, U., F. R. Jaeckel, J. Boch, M. Meyer, K. Rudolph, and M. S. Ullrich. 1998. Cloning, nucleotide sequencing and expression in Escherichia coli of levansucrase genes from the plant pathogens Pseudomonas syringae pv. glycinea and P. syringae pv. phaseolicola. Appl. Environ. Microbiol. 64:3180-3187.
16. Hugouvieux-Cotte-Pattat, N., H. Dominguez, and J. Robert-Baudouy. 1992. Environmental conditions affect transcription of the pectinase genes of Erwinia chrysanthemi 3937. J. Bacteriol. 174:7807-7818.
17. Jin, S., Y.-N. Song, W.-Y. Deng, M. P. Gordon, and E. W. Nester. 1993. The regulatory VirA protein of Agrobacterium tumefaciens does not function at elevated temperatures. J. Bacteriol. 175:6830-6835.
18. Keane, P. J., A. Kerr, and P. B. New. 1970. Crown gall of stone fruit. II. Identification and nomenclature of Agrobacterium isolates. Aust. J. Biol. Sci. 23:585-595.
19. King, E. O., M. K. Ward, and D. E. Raney. 1954. Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44:301-307.
20. Kitten, T., T. G. Kinscherf, J. L. McEvoy, and D. K. Willis. 1998. A newly identified regulator is required for virulence and toxin production in Pseudomonas syringae. Mol. Microbiol. 28:917-929.
21. Lamb, C. J., J. A. Ryals, E. R. Ward, and R. A. Dixon. 1992. Emerging strategies for enhancing crop resistance to microbial pathogens. Biotechnology 10:1436-1445.
22. Lanham, P. G., K. I. McIlravey, and M. C. M. Perombelon. 1991. Production of the cell wall dissolving enzymes by Erwinia carolovora subsp. atroseptica in vitro at 27°C and 30.5°C. J. Appl. Bacteriol. 70:20-24.
23. Miura, K., Y. Tomioka, H. Suzuki, M. Yonezawa, T. Hishinuma, and M. Mizugaki. 1997. Molecular cloning of the nemA gene encoding N-ethylmaleimide reductase from Escherichia coli. Biol. Pharm. Bull. 20:110-112.
24. O'Farrell, P. H. 1975. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250:4007-4020.
25. Palmer, D. A., and C. L. Bender. 1993. Effects of environmental and nutritional factors on production of the polyketide phytotoxin coronatine by Pseudomonas syringae pv. glycinea. Appl. Environ. Microbiol. 59:1619-1623.
26. Phillips, D. A., and Y. Kapulnik. 1995. Plant isoflavonoids, pathogens and symbionts. Trends Microbiol. 3:58-64.
27. Rangaswamy, V., M. Ullrich, W. Jones, R. Mitchell, R. Parry, P. Reynolds, and C. L. Bender. 1997. Expression and analysis of coronafacate ligase, a thermoregulated gene required for production of the phytotoxin coronatine in Pseudomonas syringae. FEBS Lett. 154:65-72.
28. Rice, P. A., A. Goldman, and T. A. Steitz. 1990. A helix-turn-strand structural motif common in alpha-beta proteins. Proteins 8:334-340.
29. Rich, J. J., T. G. Kinscherf, T. Kitten, and D. K. Willis. 1994. Genetic evidence that the gacA gene encodes the cognate response regulator for the lemA sensor in Pseudomonas syringae. J. Bacteriol. 176:7468-7475.
30. Rohde, B. H., B. Pohlack, and M. S. Ullrich. 1998. Occurrence of thermoregulation of genes involved in coronatine biosynthesis among various Pseudomonas syringae strains. J. Basic Microbiol. 38:41-50.
31. Rowley, K. B., D. E. Clements, M. Mandel, T. Humphreys, and S. S. Patil. 1993. Multiple copies of a DNA sequence from Pseudomonas syringae pathovar phaseolicola abolish thermoregulation of phaseolotoxin production. Mol. Microbiol. 8:625-635.
32. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
33. Schaller, F., and E. W. Weiler. 1997. Enzymes of octadecanoid biosynthesis in plants: 12-oxo-phytodienoate 10,11-reductase. Eur. J. Biochem. 245:294-299.
34. Schaller, F., and E. W. Weiler. 1997. Molecular cloning and characterization of 12-oxophytodienoate reductase, an enzyme of the octadecanoid signaling pathway from Arabidopsis thaliana. J. Biol. Chem. 272:28066-28072.
35. Scrutton, N. S. 1994. Alpha/beta barrel evolution and the modular assembly of enzymes: emerging trends in the flavin oxidase/dehydrogenase family. Bioessays 16:115-122.
36. Sinnott, M. 1998. Comprehensive biological catalysis: a mechanistic reference, vol. 3. Academic Press, London, England.
37. Snape, J. R., N. A. Walkley, A. P. Morby, S. Nicklin, and G. F. White. 1997. Purification, properties, and sequence of glycerol trinitrate reductase from Agrobacterium radiobacter. J. Bacteriol. 179:7796-7802.
38. Staskawicz, B. J., D. Dahlbeck, and N. T. Keen. 1984. Cloned avirulence gene of Pseudomonas syringae pv. glycinea determines race-specific incompatibility on Glycine max (L.) Merr. Proc. Natl. Acad. Sci. USA 81:6024-6028.
39. Ullrich, M., A. C. Guenzi, R. E. Mitchell, and C. L. Bender. 1994. Cloning and expression of genes required for coronamic acid (2-ethyl-1-aminocyclopropane 1-carboxylic acid), an intermediate in the biosynthesis of the phytotoxin coronatine. Appl. Environ. Microbiol. 60:2890-2897.
40. Ullrich, M., A. Penaloza-Vazquez, A. M. Bailey, and C. L. Bender. 1995. A modified two-component regulatory system is involved in temperature-dependent biosynthesis of the Pseudomonas syringae phytotoxin coronatine. J. Bacteriol. 177:6160-6169.
41. Völker, U., S. Engelmann, B. Maul, S. Rielhdorf, A. Volker, R. Schmid, H. Mach, and M. Hecker. 1994. Analysis of the induction of general stress proteins of Bacillus subtilis. Microbiology 140:741-752.
42. Wei, Z. B., and S. V. Beer. 1995. HrpL activates Erwinia amylovora hrp gene transcription and is a member of the ECF subfamily of sigma factors. J. Bacteriol. 177:6201-6210.
43. Weiler, E. W., T. M. Kutchan, T. Gorba, W. Brodschelm, U. Niesel, and F. Bublitz. 1994. The Pseudomonas phytotoxin coronatine mimics octadecanoid signalling molecules of higher plants. FEBS Lett. 345:9-13.
44. Young, S. A., S. K. Park, C. Rodgers, R. E. Mitchell, and C. L. Bender. 1992. Physical and functional characterization of the gene cluster encoding the polyketide phytotoxin coronatine in Pseudomonas syringae pv. glycinea. J. Bacteriol. 174:1837-1843.