Expression and analysis of coronafacate ligase, a thermoregulated gene required for production of the phytotoxin coronatine in Pseudomonas syringae

FEMS Microbiology Letters

Volumn 154, Issue 1, 1997, Pages 65-72

  Rangaswamy, V ^a   Ullrich, M ^a   Jones, W ^b   Mitchell, R ^b   Parry, R ^c   Reynolds, P ^b   Bender, C L ^a  

^a 127 Noble Res Ctr   (United States)

^b THE NEW ZEALAND INSTITUTE FOR PLANT AND FOOD RESEARCH LIMITED   (New Zealand)

^c RICE UNIVERSITY   (United States)

Author keywords

Phytotoxin; Pseudomonas syringae; Temperature; Thermoregulation

Indexed keywords

LIGASE; PLANT TOXIN;

ARTICLE; CATALYSIS; ENZYME ANALYSIS; GENE EXPRESSION; GENETIC ANALYSIS; IMMUNOBLOTTING; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN STRUCTURE; PSEUDOMONAS SYRINGAE; THERMOREGULATION;

AMIDE SYNTHASES; AMINO ACID SEQUENCE; AMINO ACIDS; ANTIBODY SPECIFICITY; BACTERIAL PROTEINS; BACTERIAL TOXINS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC COMPLEMENTATION TEST; IMMUNOBLOTTING; INDENES; MUTAGENESIS; PHENOTYPE; PROTEIN STRUCTURE, TERTIARY; PSEUDOMONAS; TEMPERATURE;

ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS SYRINGAE;

EID: 0030953383     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(97)00302-9     Document Type: Article

References (19)

1. Ichihara, A., Shiraishi, K., Sato, H., Sakamura, S., Nishiyama, K., Sakai, R., Furusaki, A. and Matsumoto, T. (1977) The structure of coronatine. J. Am. Chem. Soc. 99, 636-637.
2. Liyanage, H., Palmer, D.A., Ullrich, M. and Bender, C.L. (1995) Characterization and transcriptional analysis of the gene cluster for coronafacic acid, the polyketide component of the phytotoxin coronatine. Appl. Environ. Microbiol. 61, 3843-3848.
3. Liyanage, H., Penfold, C., Turner, J. and Bender, C.L. (1995) Sequence, expression, and transcriptional analysis of the coronafacate ligase-encoding gene required for coronatine biosynthesis by Pseudomonas syringae. Gene 153, 17-23.
4. Bender, C.L., Liyanage, H., Palmer, D., Ullrich, M., Young, S. and Mitchell, R. (1993) Characterization of the genes controlling biosynthesis of the polyketide phytotoxin coronatine including conjugation between coronafacic and coronamic acid. Gene 133, 31-38.
5. Keane, P.J., Kerr, A. and New, P.B. (1970) Crown gall of stone fruit. II. Identification and nomenclature of Agrobacterium isolates. Aust. J. Biol. Sci. 23, 585-595.
6. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
7. Bender, C.L., Young, S.A. and Mitchell, R.E. (1991) Conservation of plasmid DNA sequences in coronatine-producing pathovars of Pseudomonas syringae. Appl. Environ. Microbiol. 57, 993-999.
8. Palmer, D.A. and Bender, C.L. (1993) Effects of environmental and nutritional factors on production of the polyketide phytotoxin coronatine by Pseudomonas syringae pv. glycinea. Appl. Environ. Microbiol. 59, 1619-1626.
9. Riggs, P. (1994) Expression and purification of maltose-binding protein fusions In: Current Protocols in Molecular Biology (Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D., Siedman, J., Smith, J. and Struhl, K., Eds.), pp. 16.6.1-16.6.13. Wiley, New York.
10. Peñaloza-Vázquez, A., Rangaswamy, V., Ullrich, M., Bailey, A. and Bender, C. (1996) Use of translational fusions to the maltose binding protein to produce and purify proteins in Pseudomonas syringae and assess their activity in vivo. Mol. Plant-Microbe Interact. 9, 637-641.
11. Donald, R.G.K., Zhou, H. and Jackson, A.O. (1993) Serological analysis of barley stripe mosaic virus-encoded proteins in infected barley. Virology 195, 659-668.
12. Timmons, T.M. and Dunbar, B.S. (1990) Protein blotting and immunodetection. Methods Enzymol. 182, 679-688.
13. Turgay, K., Krause, M. and Marahiel, M.A. (1992) Four homologous domains in the primary structure of GrsB are related to domains in a superfamily of adenylate-forming enzymes. Mol. Microbiol. 6, 529-546.
14. Hamoen, L.W., Eshuis, H., Jongbloed, J., Venema, G. and van Sinderen, D. (1995) A small gene, designated comS, located within the coding region of the fourth amino acid-activation domain of srfA, is required for competence development in Bacillus subtilis. Mol. Microbiol. 15, 55-63.
15. Hutchinson, C.R. and Fujii, I. (1995) Polyketide synthase gene manipulation: A structure-function approach in engineering novel antibiotics. Annu. Rev. Microbiol. 49, 201-38.
16. Lowden, P., Böhm, G., Staunton, J. and Leadlay, P.F. (1996) The nature of the starter unit for the rapamycin polyketide synthase. Angew. Chem. Int. Ed. Engl. 35, 2249-2251.
17. Parry, R.J., Jiralerspong, S., Mhaskar, S., Alemany, L. and Wilcott, R. (1996) Investigations of coronatine biosynthesis. Elucidation of the mode of incorporation of pyruvate into coronafacic acid. J. Am. Chem. Soc. 118, 703-704.
18. Ullrich, M., Guenzi, A., Mitchell, R. and Bender, C. (1994) Cloning and expression of genes required for coronamic acid (2-ethyl-1-aminocyclopropane 1-carboxylic acid), an intermediate in the biosynthesis of the phytotoxin coronatine. Appl. Environ. Microbiol. 60, 2890-2897.
19. Murillo, J., Shen, H., Gerhold, D., Sharma, A., Cooksey, D.A. and Keen, N.T. (1994) Characterization of pPT23B, the plasmid involved in syringolide production by Pseudomonas syringae pv. tomato PT23. Plasmid 31, 275-287.