Chinese hamster ovary cells produce sufficient recombinant insulin-like growth factor I to support growth in serum-free medium

Cytotechnology

Volumn 24, Issue 1, 1997, Pages 55-64

  Hunt, S M N ^a,d   Pak, S C O ^a   Bridges, M W ^a   Gray, P P ^a   Sleigh, M J ^b,c  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

^b CSIRO   (Australia)

^c Peptide Technology Ltd   (Australia)

^d WESTMEAD HOSPITAL   (Australia)

Author keywords

autocrine growth; cell culture; CHO; IGF I; serum free

Indexed keywords

EID: 0031003396     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1007969502256     Document Type: Article

References (34)

1. Ballard FJ, Francis GL, Ross M, Bagley CJ, May B and Wallace JC (1987) Natural and synthetic forms of insulin-like growth factor-1 (IGF-1) and the potent derivative, destripeptide IGF-1: biological activities and receptor binding. Biochem. Biophys. Res. Commun. 149: 398-404.
2. Ballotti R, Nielsen FC, Pringle N, Kowalski A, Richardson WD, Van Obberghen E and Gammeltoft S (1987) Insulin-like growth factor I in cultured rat astrocytes: expression of the gene, and receptor tyrosine kinase. Embo. J. 6: 3633-3639.
3. Barnes D and Sato G (1980) Methods for growth of cultured cells in serum-free medium. Anal. Biochem. 102: 255-270.
4. Baxter RC (1986) The somatomedins: insulin-like growth factors. Adv. Clin. Chem. 25: 49-115.
5. Bayne ML, Applebaum J, Chicchi GG, Hayes NS, Green BG and Cascieri MA (1988) Expression, purification and characterization of recombinant human insulin- like growth factor I in yeast. Gene 66: 235-244.
6. Bayne ML, Cascieri MA, kelder B, Applebaum J, Chicchi GG, Shapiro JA, Pasleau F and Kopchick JJ (1987) Expression of a synthetic gene encoding human insulin-like growth factor 1 in cultured mouse fibroblasts. Proc. Natl. Acad. Sci. U.S.A. 84: 2638-2642.
7. Bendig MM (1988) The production of foreign proteins in mammalian cells. Genet. Eng. 7: 91-127.
8. Blum WF, Jenne EW, Reppin F, Kietzmann K, Ranke MB and Bierich JR (1989) Insulin-like growth factor I (IGF-I)-binding protein complex is a better mitogen than free IGF-I. Endocrinology 125: 766-772.
9. Bovenberg WA, Dauwerse JG, Pospiech HM, Van Buul Offers SC, Van den Brande JI and Sussenbach JS (1990) Expression of recombinant human insulin-like growth factor I in mammalian cells. Mol. Cell. Endocrinol. 74: 45-59.
10. Cascieri MA, Hayes NS, Kelder B, Kopchick JJ, Chicchi GG, Slater EE and Bayne ML (1988) Inability of a mouse cell line transformed to produce biologically active recombinant human insulin-like growth factor I (IGF-I) to respond to exogenously added IGF-I. Endocrinology 122: 1314-1320.
11. Chen C and Okayama H (1988) Calcium phosphate-mediated gene transfer: A highly effective system for stably transforming cells with plasmid DNA. Bio/Techniques 6: 632-638.
12. Clemmons DR (1984) Multiple hormones stimulate the production of somatomedin by cultured human fibroblasts. J. Clin. Endocrinol. Metab. 58: 850-856.
13. Clemmons DR and Shaw DS (1983) Variables controlling somatomedin production by cultured human fibroblasts. J. Cell. Physiol. 115: 137-142.
14. Clemmons DR, Underwood LE and Van Wyk JJ (1981) Hormonal control of immunoreactive somatomedin production by cultured human fibroblasts. J. Clin. Invest. 67: 10-19.
15. Dai Z, Stiles AD, Moats SB, Van Wyk JJ and D'Ercole AJ (1992a) Interaction of secreted insulin-like growth factor-I (IGF-I) with cell surface receptors is the dominant mechanism of IGF-I's autocrine actions. J. Biol. Chem. 267: 19565-19571.
16. Dai Z, Takahashi SI, Van Wyk JJ and D'Ercole AJ (1992b) Creation of an autocrine model of insulin-like growth factor-I action in transfected FRTL-5 cells. Endocrinology 130: 3175-3183.
17. Daughaday WH and Rotwein P (1989) Insulin-like growth factors I and II. Peptide, messenger ribonucleic acid and gene structures, serum, and tissue concentrations. Endocr. Rev. 10: 68-91.
18. Friedman JS, Cofer CL, Anderson CL, Kushner JA, Gray PP, Chapman GE, Stuart MC, Lazarus L, Shine J and Kushner PJ (1989) High expression in mammalian cells without amplification. Bio/Technology 7: 359-362.
19. Friedman KD, Rosen NL, Newman PJ and Montgomery RR (1988) Enzymatic amplification of specific cDNA inserts from lambda gt11 libraries. Nucleic Acids Res. 16: 8718.
20. Froesch ER, Schmid C, Schwander J and Zapf J (1985) Actions of insulin-like growth factors. Ann. Rev. Physiol. 443-467.
21. Gough NM (1988) Rapid and quantitative preparation of cytoplasmic RNA from small numbers of cells. Anal. Biochem. 173: 93-95.
22. Jansen M, van Schaik FMA, Ricker AT, Bullock B, Woods DE, Gabbay KH, Nussbaum AL, Sussenbach JS and Van den Brande JL (1983) Sequence of cDNA encoding human insulin-like growth factor I precursor. Nature 306: 609-611.
23. Kelly JL, Sanchez A, Brown GS, Chesterman CN and Sleigh MJ (1993) Accumulation of PDGFB and cell-binding forms of PDGFA in the extracellular matrix. J. Cell. Biol. 121: 1153-1163.
24. Kozak M (1986) Point Mutations Define a Sequence Flanking the AUG initiator Codon that Modulates Translation by Eukaryotic Ribosomes. Cell 44: 283-292.
25. Laemmli UK (1970) Cleavage of structural protein during assembly of the head bacteriophage T4. Nature 227: 680-685.
26. Laub O and Rutler WJ (1983) Expression of the human insulin gene and cDNA in a heterologous mammalian system. J. Biol. Chem. 258: 6043-6050.
27. Maniatis T, Fritsch EF and Sambrook J. (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, New York.
28. McKinnon P, Ross M, Wells JR, Ballard FJ and Francis GL (1991) Expression, purification and characterization of secreted recombinant human insulin-like growth factor-I (IGF-I) and the potent variant des(1-3) IGF-I in Chinese hamster ovary cells. J. Mol. Endocrinol. 6: 231-239.
29. Moore HP, Walker MD, Lee F and Kelly RB (1983) Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation. Cell 35: 531-538.
30. Rechler MM and Nissley SP (1985) The nature and regulation of the receptors for insulin-like growth factors. Annu. Rev. Physiol. 47: 425-442.
31. Ross S and Englesberg E (1993) The competence progression model in CHO-K1 cells: the relationship between protein kinase C and immediate early gene expression in the insulin mitogenic signal. Biochim. Biophys. Acta 1177: 307-317.
32. Rotwein P, Pollock KM, Didier DK and Krivi GG (1986) Organization and sequence of the human insulin-like growth factor I gene. Alternative RNA processing produces two insulin-like growth factor I precursor peptides. J. Biol. Chem. 261: 4828-4832.
33. Vollenweider F, Irminger JC, Gross DJ, Villa KL and Halban PA (1992) Processing of proinsulin by transfected hepatoma (FAO) cells. J. Biol. Chem. 267: 14629-14636.
34. Vollenweider F, Irminger JC and Halban PA (1993) Substrate specificity of proinsulin conversion in the constitutive pathway of transfected FAO (hepatoma) cells. Diabetologia 36: 1322-1325.