Detection and quantification of Salmonella in pure cultures using 5'- nuclease polymerase chain reaction

International Journal of Food Microbiology

Volumn 51, Issue 2-3, 1999, Pages 191-196

  Nogva, Hege Karin ^a   Lillehaug, Dag ^a  

^a NORWEGIAN FOOD RESEARCH INSTITUTE   (Norway)

Author keywords

5' Nuclease PCR; Detection; Quantification; Salmonella

Indexed keywords

5' NUCLEOTIDASE; BACTERIAL DNA; BACTERIAL RNA;

ARTICLE; AUTOMATION; BACTERIAL COUNT; BACTERIUM CULTURE; BACTERIUM DETECTION; FOOD CONTAMINATION; NONHUMAN; POLYMERASE CHAIN REACTION; SALMONELLA;

COLONY COUNT, MICROBIAL; ENDODEOXYRIBONUCLEASES; EXODEOXYRIBONUCLEASE V; EXODEOXYRIBONUCLEASES; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; SALMONELLA;

EID: 0032881753     PISSN: 01681605     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1605(99)00122-1     Document Type: Article

References (13)

1. Bassler H.A., Flood S.J.A., Livak K.J., Marmaro J., Knorr R., Batt C.A. Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes. Appl. Environ. Microbiol. 61:1995;3724-3728.
2. Candrian U. Polymerase chain reaction in food microbiology. J. Microbiol. Methods. 23:1995;89-103.
3. Chen S., Yee A., Griffiths M., Larkin C., Yamashiro C.T., Behari R., Paszko-Kolva C., Rahn K., De Grandis S.A. The evaluation of a fluorogenic polymerase chain reaction assay for the detection of Salmonella species in food commodities. Int. J. Food. Microbiol. 35:1997;239-250.
4. D'Aoust J.Y., Pivnick H. Small infectious doses of Salmonella. Lancet. 1:1972;866.
5. Guttorm, A., 1998. Sammenligning av hurtigmetoder for bestemmelse av Salmonella spp. i næringsmidler og renkultur. Thesis, INF, Agricultural University of Norway, Ås, Norway.
6. Heid C.A., Stevens J., Livak K.J., Williams P.M. Real time quantitative PCR. Genome Res. 6:1996;986-994.
7. Holland P.M., Abramson R.D., Watson R., Gelfand D.H. Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA. 88:1991;7276-7280.
8. Jay, J.M., 1996. Foodborne Gastroenteritis caused by Salmonella and Shigella. In: Modern Food Microbiology, 5th ed., Chapman & Hall, New York, p. 514.
9. Livak K.J., Flood S.J.A., Marmaro J., Giusti W., Deetz K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl. 4:1995;357-362.
10. Olsen J.E., Aabo S., Hill W., Notermans S., Wernars K., Granum P.E., Popovic T., Rasmussen H.N., Olsvik O. Probes and polymerase chain reaction for detection of food-borne bacterial pathogens. Int. J. Food Microbiol. 28:1995;1-78.
11. Perkin-Elmer Applied Biosystems, 1997. User Bulletin 2; ABI PRISM 7700 Sequence Detection System.
12. Raeymaekers L. Quantitative PCR: Theoretical considerations with practical implications. Anal. Biochem. 214:1993;582-585.
13. Wang R.-F., Cao W.-W., Cerniglia C.E. A universal protocol for PCR detection of 13 species of foodborne pathogens in foods. J. Appl. Microbiol. 83:1997;727-736.