Homogeneous noncompetitive immunoassay based on the energy transfer between fluorolabeled antibody variable domains (open sandwich fluoroimmunoassay)

BioTechniques

Volumn 27, Issue 4, 1999, Pages 738-742

  Ueda, Hiroshi ^a,d   Kubota, K ^a   Wang, Y ^a   Tsumoto, K ^b   Mahoney, W ^c   Kumagai, I ^b   Nagamune, T ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

^c Roche Diagnostics   (United States)

^d MEDICAL RESEARCH COUNCIL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME ANTIBODY; RHODAMINE; SUCCINIMIDE DERIVATIVE;

ARTICLE; CONCENTRATION (PARAMETERS); ENERGY TRANSFER; FLUOROIMMUNOASSAY; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; NONHUMAN; SPECTROFLUOROMETRY;

EID: 0032875188     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99274st04     Document Type: Article

References (18)

1. Anderson, F.P. and W.G. Miller. 1988. Fiber optic immunochemical sensor for continuous, reversible measurement of phenytoin. Clin. Chem. 45:1417-1421.
2. Geoghegan, K.F. 1998. Chemical modification of amino group, p. 15.2.1. In J.E. Coligan, B.M. Dunn, H.L. Ploegh, D.W. Speicher and P.T. Wingfield (Eds.), Current Protocols in Protein Science. John Wiley & Sons, New York.
3. Glockshuber, R., M. Malia, I. Pfitzinger and A. Plückthun. 1990. A comparison of strategies to stabilize immunoglobulin Fv-fragments. Biochemistry 29:1362-1367.
4. Jager, M. and A. Plückthun. 1999. Folding and assembly of an antibody Fv fragment, a heterodimer stabilized by antigen. J. Mol. Biol. 255:2005-2019.
5. Kronick, M.N. and P.D. Grossman. 1983. Immunoassay techniques with fluorescent phycobiliprotein conjugates. Clin. Chem. 29: 1582-1586.
6. Mathis, G. 1993. Rare earth cryptates and homogeneous fluoroimmunoassays with human sera. Clin. Chem. 39:1953-1959.
7. Patel, L.R., T. Curran and T.K. Kerppola. 1994. Energy transfer analysis of Fos-Jun dimerization and DNA binding. Proc. Natl. Acad. Sci. USA 91:7360-7364.
8. Selvin, P.R. 1995. Fluorescence resonance energy transfer. Methods Enzymol. 246:300-334.
9. Selvin, P.R. and J.E. Hearst. 1994. Luminescence energy transfer using a terbium chelate: improvements on fluorescence energy transfer. Proc. Natl. Acad. Sci. USA 91:10024-10028.
10. L-alkaline phosphatase fusion proteins. J. Immunol. Methods 224:171-184.
11. Tsumoto, K., Y. Nishimiya, N. Kasai, H. Ueda, T. Nagamune, K. Ogasahara, K. Yutani, K. Tokuhisa, et al. 1997. Novel selection method for engineered antibodies using the mechanism of Fv fragment stabilization in the presence of antigen. Protein Eng. 10:1311-1318.
12. Tsumoto, K., Y. Ueda, K. Maenaka, K. Watanabe, K. Ogasawara, K. Yutani and I. Kumagal. 1994. Contribution to antibody-antigen interaction of structurally perturbed antigenic residues upon antibody binding. J. Biol. Chem. 269:28777-28782.
13. Uchiyama, H., K. Hirano, M. Kashiwasake-Jibu and K. Taira. 1996. Detection of undegraded oligonucleotides in vivo by fluorescence resonance energy transfer. Nuclease activities in living sea urchin eggs. J. Biol. Chem. 271:380-384.
14. Ueda, H., K. Tsumoto, K. Kubota, E. Suzuki, T. Nagamune, H. Nishimura, P.A. Schueler, G. Winter, I. Kumagai and W.C. Mahoney. 1996. Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region. Nature Biotechnol. 14:1714-1718.
15. Ueda, Y., K. Tsumoto, K. Watanabe and I. Kumagai. 1993. Synthesis and expression of a DNA encoding the Fv domain of an anti-lysozyme monoclonal antibody, HyHEL10, in Streptomyces lividans. Gene 129:129-134.
16. Ullman, E.F., M. Schwarzberg and K.E. Rubenstein. 1976. Fluorescent excitation transfer immunoassay. A general method for determination of antigens. J. Biol. Chem. 257:4172-4178.
17. Wu, P. and L. Brand. 1994. Resonance energy transfer methods and applications. Anal. Biochem. 218:1-13.
18. Youn, H.J., E. Terpetschnig, H. Szmacinski and J.R. Lakowicz. 1995. Fluorescence energy transfer immunoassay based on a long-life-time luminescent metal-ligand complex. Anal. Biochem. 232:24-30.