New ribosome-inactivating proteins from seeds and fruits of the bitter gourd Momordica charantia

International Journal of Biochemistry and Cell Biology

Volumn 31, Issue 9, 1999, Pages 895-901

  Tse, Paul M F ^a   Ng, T B ^a,b   Fong, W P ^a   Wong, R N S ^b   Wan, C C ^b   Mak, N K ^b   Yeung, H W ^a  

^a and Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics   (Hong Kong)

^b HONG KONG BAPTIST UNIVERSITY   (Hong Kong)

Author keywords

Bitter gourd; Momorcharins; Ribosome inactivating protein

Indexed keywords

ALPHA MOMORCHARIN; BETA MOMORCHARIN; DELTA MOMORCHARIN; EPSILON MOMORCHARIN; MOMORCHARIN; MOMORDICA CHARANTIA EXTRACT; RIBOSOME INACTIVATING PROTEIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; FAST PROTEIN LIQUID CHROMATOGRAPHY; FRUIT; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PLANT SEED; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS INHIBITION; RABBIT; RETICULOCYTE LYSATE; RNA TRANSLATION;

EID: 0032875102     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1357-2725(99)00039-4     Document Type: Article

References (18)

1. L. Barbieri, M.G. Battelli, F. Stirpe, Ribosome-inactivating proteins from plants, Biochim. Biophys. Acta 1154 (1993) 237-282.
2. T. Girbes, L. Citores, R. Iglesias, J.M. Ferreras, R. Munoz, M.A. Rojo, F.J. Arias, J.R. Garcia, E. Mendez, Ebulin 1, a nontoxic type 2 ribosome-inactivating protein from Sambucus ebulus L. leaves, J. Biol. Chem. 268 (1993) 18195-18199.
3. T. Girbes, L. Citores, J.M. Ferreras, M.A. Rojo, R. Iglesias, R. Munoz, F.J. Arias, M. Calonge, J.R. Garcia, E. Mendez, Isolation and partial characterization of nigrin b, a nontoxic novel type 2 ribosome-inactivating protein from the bark of Sambucus nigra L, Plant Mol. Biol. 22 (1993) 1181-1186.
4. W. Gao, J. Ling, X. Zhong, W. Liu, R. Zhang, H. Yang, H. Cao, Z. Zhang, Luffin S: a small novel ribosome-inactivating protein from Luffa cylindrica, FEBS Lett. 347 (1994) 257-260.
5. Z. Pu, B.Y. Lu, W.Y. Liu, S.W. Jin, Characterization of the enzymatic mechanism of γ-momorcharin, a novel ribosome-inactivating protein with lower molecular weight of 11,500 purified from the seeds of bitter gourd Momordica charantia, Biochem. Biophys. Res. Commun. 229 (1996) 287-294.
6. R.B. Pelham, R.J. Jackson, An efficient mRNA-dependent translation system from rabbit reticulocyte lysates, Eur. J. Biochem. 67 (1976) 227-256.
7. T.T.M. Go, H.W. Yeung, W.P. Fong, Deoxyribonucleolytic activity of α- and β-momorcharins, Life Sci. 51 (1992) 1347-1353.
8. J.W.Y. Mock, T.B. Ng, R.N.S. Wong, Q.Z. Yao, H.W. Yeung, W.P. Fong, Demonstration of ribonuclease ac-tivity in the plant ribosome-inactivating proteins alpha- and beta-momorcharins, Life Sci. 59 (1996) 1853-1859.
9. T.B. Ng, W.Y. Chan, H.W. Yeung, Proteins with abortifacient, ribosome inactivating, immunomodulatory, antitumor and antiAIDS activities from Cucurbitaceae plants, Gen. Pharmac. 23 (1992) 575-590.
10. H.W. Yeung, W.W. Li, W.Y. Chan, L.K. Law, T.B. Ng, Alpha- and beta-momorcharins: abortifacient proteins from the seeds of the bitter gourd Momordlca charantia (Family Cucurbitaceae), Int. J. Pept. Protein Res. 28 (1986) 518-524.
11. S. Lee-Huang, P.L. Huang, P.L. Nara, H.C. Chen, H.F. Kung, P. Huang, H.I. Huang, P.L. Huang, MAP 30: a new inhibitor of HIV-1 infection and replication, FEBS Lett. 272 (1990) 12-18.
12. O.H. Lowry, N.J. Rosebrough, A.L. Farr, R.J. Randall, Protein measurement with the folin phenol reagent, J. Biol. Chem. 193 (1951) 265-275.
13. U.K. Laemmli, M. Favre, Maturation of the head of bacteriophage T4. I. DNA packaging events, J. Mol. Biol. 80 (1973) 575-599.
14. Y. Endo, K. Mitsui, M. Motizuki, K. Tsurugi, The mechanism of ricin and related toxins on eukaryotic ribosomes, J. Biol. Chem. 262 (1987) 5908-5912.
15. W.P. Fong, Y.T. Poon, T.M. Wong, J.W.Y. Mock, T.B. Ng, R.N.S. Wong, Q.Z. Yao, H.W. Yeung, A highly efficient procedure for purifying the ribosomeinactivating proteins α- and β-momorcharins from Momordica charantia seeds, N-terminal sequence comparison and establishment of their N-glycosidase activity, Life Sci. 59 (1996) 901-909.
16. W.K.K. Ho, S.C. Liu, P.C. Shaw, H.W. Yeung, T.B. Ng, W.Y. Chan, Cloning of the cDNA of α-momorcharin: a ribosome inactivating protein, Biochim. Biophys. Acta 1088 (1991) 311-314.
17. J.M. Ferraras, L. Barbieri, T. Girbes, M.G. Battelli, M.A. Rojo, F.J. Arian, M.A. Rocher, F. Soriano, E. Mendez, F. Stirpe, Distribution and properties of major ribosome-inactivating proteins (2851 rRNA N-glycosidases) of the plant Saponaria officinalis L. (Caryophyllaceae), Biochim. Biophys. Acta 1216 (1993) 31-42.
18. S. Lee-Huang, P.L. Huang, H.C. Chen, P.L. Huang, A. Bourinbaiar, H.L. Huang, H.F. Kung, AntiHIV and antitumor activities of recombinant MAP 30 from bitter melon, Gene 161 (1995) 151-156.