Purification and characterization of outer membrane protein P6, a vaccine antigen of non-typeable Haemophilus influenzae

FEMS Immunology and Medical Microbiology

Volumn 26, Issue 2, 1999, Pages 159-166

  Karalus, Richard J ^a   Murphy, Timothy F ^a,b,c  

^a UNIVERSITY AT BUFFALO   (United States)

^b UNIVERSITY AT BUFFALO   (United States)

^c DEPARTMENT OF VETERANS AFFAIRS   (United States)

Author keywords

Bacterial vaccine; Haemophilus influenzae; Outer membrane protein; Protein purification

Indexed keywords

HAEMOPHILUS INFLUENZAE VACCINE; OUTER MEMBRANE PROTEIN;

ANTIGENICITY; ARTICLE; CIRCULAR DICHROISM; HAEMOPHILUS INFLUENZAE; IMMUNOGENICITY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE;

ADULT; ANTIBODIES, MONOCLONAL; ANTIGENS, BACTERIAL; BACTERIAL OUTER MEMBRANE PROTEINS; CIRCULAR DICHROISM; HAEMOPHILUS INFLUENZAE; HAEMOPHILUS VACCINES; HUMANS; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SPUTUM;

EID: 0032873619     PISSN: 09288244     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0928-8244(99)00136-4     Document Type: Article

References (28)

1. Klein J.O. Otitis media. Clin. Infect. Dis. 19:1994;823-833.
2. Kaplan B., Wandstrat T.L., Cunningham J.R. Overall cost in the treatment of otitis media. Pediatr. Infect. Dis. J. 16:1997;S9-S11.
3. Murphy T.F., Sethi S. Bacterial infection in chronic obstructive pulmonary disease. Am. Rev. Respir. Dis. 146:1992;1067-1083.
4. Murphy T.F., Sethi S. A national strategy for research in chronic obstructive pulmonary disease. JAMA. 277:1997;1596.
5. Munson R.S. Jr., Granoff D.M. Purification and partial characterization of outer membrane proteins P5 and P6 from Haemophilus influenzae type b. Infect. Immun. 49:1985;544-549.
6. Murphy T.F., Bartos L.C., Campagnari A.A., Nelson M.B., Apicella M.A. Antigenic characterization of the P6 protein of nontypable Haemophilus influenzae. Infect. Immun. 54:1986;774-779.
7. Murphy T.F., Nelson M.B., Dudas K.C., Mylotte J.M., Apicella M.A. Identification of a specific epitope of Haemophilus influenzae on a 16 600-dalton outer membrane protein. J. Infect. Dis. 152:1985;1300-1307.
8. Nelson M.B., Munson R.S. Jr., Apicella M.A., Sikkema D.J., Molleston J.P., Murphy T.F. Molecular conservation of the P6 outer membrane protein among strains of Haemophilus influenzae: analysis of antigenic determinants, gene sequences, and restriction fragment length polymorphisms. Infect. Immun. 59:1991;2658-2663.
9. Beck-Sickenger A.G., Rotering H., Weismuller K.H., Dorner F., Jung G. Mapping of antigenic and immunogenic sites of Haemophilus influenzae outer membrane protein P6 using synthetic lipopeptides. Biol. Chem. Hoppe-Seyler. 375:1994;173-182.
10. Bogdan J.A. Jr., Apicella M.A. Mapping of a surface-exposed, conformational epitope of the P6 protein of Haemophilus influenzae. Infect. Immun. 63:1995;4395-4401.
11. Nelson M.B., Apicella M.A., Murphy T.F., Vankeulen H., Spotila L.D., Rekosh D. Cloning and sequencing of Haemophilus influenzae outer membrane protein P6. Infect. Immun. 56:1988;128-134.
12. Murphy T.F., Bartos L.C., Rice P.A., Nelson M.B., Dudas K.C., Apicella M.A. Identification of a 16 600-dalton outer membrane protein on nontypable Haemophilus influenzae as a target for human serum bactericidal antibody. J. Clin. Invest. 78:1986;1020-1027.
13. DeMaria T.F., Murwin D.M., Leake E.R. Immunization with outer membrane protein P6 from nontypeable Haemophilus influenzae induces bactericidal antibody and affords protection in the chinchilla model of otitis media. Infect. Immun. 64:1996;5187-5192.
14. Kyd J.M., Dunkley M.L., Cripps A.W. Enhanced respiratory clearance of nontypeable Haemophilus influenzae following mucosal immunization with P6 in a rat model. Infect. Immun. 63:1995;2931-2940.
15. Green B.A., Metcalf B.J., Quinn-Dey T., Kirkley D.H., Quataert S.A., Deich R.A. A recombinant non-fatty acylated form of the Hi-PAL (P6) protein of Haemophilus influenzae elicits biologically active antibody against both nontypeable and type b H. influenzae. Infect. Immun. 58:1990;3272-3278.
16. Yamanaka N., Faden H. Antibody response to outer membrane protein of nontypeable Haemophilus influenzae in otitis-prone children. J. Pediatr. 122:1993;212-218.
17. Harabuchi Y., Faden H., Yamanaka N., Duffy L., Wolf J., Krystofik D. Human milk secretory IgA antibody to nontypeable Haemophilus influenzae: Possible protective effects against nasopharyngeal colonization. J. Pediatr. 124:1994;193-198.
18. Harabuchi Y., Faden H., Yamanaka N., Duffy L., Wolf J., Krystofik D. Nasopharyngeal colonization with nontypeable Haemophilus influenzae and recurrent otitis media. J. Infect. Dis. 170:1994;862-866.
19. Haase E.M., Yi K., Morse G.D., Murphy T.F. Mapping of bactericidal epitopes on the P2 porin protein of nontypeable Haemophilus influenzae. Infect. Immun. 62:1994;3712-3722.
20. Murphy T.F., Bartos L.C. Human bactericidal antibody response to outer membrane protein P2 of nontypeable Haemophilus influenzae. Infect. Immun. 56:1988;2673-2679.
21. Laemmli U.K. Cleavage of the structural proteins during assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
22. Towbin H., Staehelin T., Gorden J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350.
23. Kennett R.H. Cell fusion. Methods Enzymol. 58:1979;345-359.
24. Perczell A., Park K., Fasman G.D. Analysis of the circular dichroism spectrum of proteins using convex constraint analysis algorithm: A practical guide. Anal. Biochem. 203:1992;83-93.
25. Tsai C.M., Frasch C.E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Med. Biochem. 119:1982;115-119.
26. Murphy T.F., Kirkham C., Sikkema D.J. Neonatal, urogenital isolates of biotype 4 nontypeable Haemophilus influenzae express a variant P6 outer membrane protein molecule. Infect. Immun. 60:1992;2016-2022.
27. Zlotnick G.W., Sanfilippo V.T., Mattler J.A., Kirkley D.H., Boykins R.A., Seid R.C. Jr. Purification and characterization of a peptidoglycan-associated lipoprotein from Haemophilus influenzae. J. Biol. Chem. 263:1988;9790-9794.
28. Yang Y.-P., Munson R.S. Jr., Grass S., Chong P., Harkness R.E., Gisonni L., James O., Kwok Y., Klein M.H. Effect of lipid modification on the physicochemical, structural, antigenic and immunoprotective properties of Haemophilus influenzae outer membrane protein P6. Vaccine. 15:1998;976-987.